Office of Science

Cell Isolation and Systems Analysis

The Cell Isolation & Systems Analysis (CISA) capability at EMSL includes techniques for isolating cells from complex cell populations or environmental samples for further 'omics and imaging analyses. EMSL specializes in quantitative live cell fluorescence imaging with single molecule sensitivity, super resolution fluorescence and electron microscopy techniques, and transcriptomics and proteomics analyses. These capabilities provide the foundation for attaining a molecular-level understanding of protein functions and microbial-community dynamics to enable molecular level investigations of single cells and the pursuit of systems biology.

Capability Detail

Photo of researcher in laboratory
  1. Live Cell Chemical Profiling of Temporal Redox Dynamics in a Photoautotrophic Cyanobacterium.
  2. Three Human Cell Types Respond to Multi-Walled Carbon Nanotubes and Titanium Dioxide Nanobelts with Cell-Specific Transcriptomic and Proteomic Expression Patterns.
  3. Lithium Metal Anodes for Rechargeable Batteries.
  4. Characterization and modeling of the cemented sediment surrounding the Iulia Felix glass.
  5. High-energy radiation damage in zirconia: modeling results .
  1. Targeted strategies improve efficacy of enzymes to convert biomass to biofuels (Enzyme evaluation)
  2. Iron-bearing minerals in sediments naturally reduce contaminant levels (Lack of iron)
  3. SCF1 frees plant sugars in lignin for sustainable biofuels (Biofuel breakdown)
  4. Predictive model a step toward using bacteria as a renewable fuel source (Green isoprene)
  5. Examining the core components of Arctic clouds to clear up their influence on climate (Forecast calls for better models)

Cell Isolation and Systems Analysis Capabilities Available at EMSL

To help with proposal planning, icons in the table below indicate instrument availability:

  • 10 hours a day, 5 days a week
  • 24 hours a day, 7 days a week
Instrument Contact
Markillie,Lye Meng
Orr, Galya
  • CyTOF - Mass Cytometry
Orr, Galya
Arey, Bruce
Dohnalkova, Alice
Evans, James
Chrisler,William B
Orr, Galya
Hu, Dehong
Orr, Galya
  • Microbial Bioreactors
Orr, Galya
Hu, Dehong
Orr, Galya
Hu, Dehong
Orr, Galya
Hu, Dehong
Orr, Galya
Orr, Galya
Jiang, Weilin
Lea, Scott
Shutthanandan, Shuttha
Hu, Dehong
Cell Isolation and Systems Analysis Capability Lead: Galya Orr | , 509-371-6127