Microscopy

Advancement in energy, environment and biology research relies heavily on micro-, nano- and atomic-scale chemical and structural imaging. Many microscopy instruments have high-resolution imaging capabilities including complementary chemical, structural and phase information, in-situ imaging in native environments and imaging of dynamic processes with high temporal-resolution. See a complete list of Microscopy instruments.

Resources and Techniques

  • Nanoscale and sub-nanoscale imaging allows users to elucidate chemical processes and acquire structural data for a variety of samples such as nanostructures and cell-surface proteins.
  • Tomography yields three-dimensional reconstruction of transmission electron microscopy images generated for biological samples as well as for soft materials and samples with 3D structural heterogeneity.
  • Environmental particle analysis offers knowledge about non-volatile atmospheric particle composition and hydration properties using high-pressure scanning electron microscopy equipped with energy-dispersive x-ray analysis capability.
  • Environmental mode imaging techniques enable sample preservation to eliminate extensive preparation procedures that can introduce artifacts and make possible live-cell imaging and in situ imaging in liquids or controlled gas environments with high resolution microscopy.
  • Dynamic imaging capability enables real-time studies of nanosecond-scale dynamic processes with unprecedented spatial resolution, such as protein-protein interactions, with contrast at the single-molecule level.

Quiet Wing for Advanced Microscopy
Seven microscopes are housed in the Quiet Wing, a space specially designed to reduce external factors, such as vibrations and electromagnetic fields, that can impede capture of high-resolution images. Read more about the Quiet Wing and its instrumentation.

A new DTEM – Dynamic Transmission Electron Microscope – is under development at EMSL in collaboration with scientific colleagues at Pacific Northwest National Laboratory. It will be housed in the Quiet Wing. To learn more about this system, the science it will advance and its historical development, visit the DTEM page.

Description

Capability Details

• Electron microscopes with tomography, cryo, scanning, photoemission and high-resolution (sub-nanometer) imaging capabilities
• Focused ion beam/scanning electron microscopes for specialized sample preparation and three-dimensional topographic and chemical imaging
• Nuclear magnetic resonance microscopy with 10-40-_m resolution to study the anatomy, metabolism and transport processes of live cell cultures, biofilms and tissue samples
• Dual Raman confocal microscope for analysis of radiological samples
• Single-molecule fluorescence tools to study molecular interactions in real time
• Scanning probe microscopy with capabilities ranging from examination of dynamic nanoscale processes in condensed environments to high resolution studies of catalysis materials in ultra-high vacuum.

 

Instruments

EMSL's Digital Instruments Radiological BioScope™ Atomic Force Microscope (AFM) allows radiological samples to be examined in fluids or air with...
Custodian(s): Kevin M. Rosso
The LEAP® 4000 XHR local electrode atom probe tomography instrument enabled the first-ever comprehensive and accurate 3-D chemical imaging studies...
Custodian(s): Arun Devaraj, Daniel Perea
This FEI Tecnai T-12 cryo-transmission electron microscope (TEM) complements EMSL's broader microscopy suite and JEOL 2010 analytical high-...
Custodian(s): Alice Dohnalkova
The Asylum MFP-3D BIO is a versatile atomic force microscope (AFM) that combines molecular resolution imaging and picoNewton force-based...
Custodian(s): Kevin M. Rosso
Housed in EMSL's RadEMSL (Radiochemistry Annex), the field emission electron microprobe (EMP) enables chemical analysis and imaging of radionuclides...
Custodian(s): Bruce Arey

Publications

14YWT oxide dispersion strengthened (ODS) ferritic steel was irradiated with of 5 MeV Ni2+ ions, at 300 °C, 450 °C, and 600 °...
Melanoma is a malignant tumor of melanocytes. Although extensive investigations have been done to study metabolic changes in primary melanoma in...
Grain growth of nanocrystalline materials is generally thermally activated, but can also be driven by irradiation at much lower temperature. In...
Dual beam depth profiling strategy has been widely adopted in ToF-SIMS depth profiling, in which two basic operation modes, interlaced mode and non-...
The microstructure and chemistry of SmCo2Fe2B melt-spun alloy after multistage annealing was investigated using high resolution transmission electron...

Science Highlights

Posted: July 06, 2015
Industry uses zeolites to convert oil to gasoline, but the exact chemical transformations occurring within zeolites is not well understood. An...
Posted: April 15, 2015
Replacing internal combustion engines with fuel cells is not currently feasible because the cells use platinum-based catalysts, and platinum is a...
Posted: April 14, 2015
The Science Scientists found the incorporation of neptunium (V) (NpO2+, neptunyl) and uranium (VI) (UO22+, uranyl) in a variety of mineral...
Posted: March 30, 2015
The Science Lipids derived from oil-rich microorganisms such as bacteria, yeast and microalgae offer a promising source of renewable fuels and...
Posted: March 24, 2015
To understand a lithium battery at the nanoscale, scientists with EMSL and other organizations at the Department of Energy’s Joint Center for Energy...

Instruments

The first goal of this research program is to develop a robust method to quantify the atomic scale changes in structure, composition and bonding that...
The goal of this proposal is to understand the microstructural evolution during non-equilibrium, rapid solidification of a molten magnesium-aluminum-...
Biomass burning aerosols (BBA) are one of the most ubiquitous organic aerosol types with a source strength on the same order of magnitude as fossil...
Development and deployment of low enriched uranium (LEU) fuels has been pursued for more than 30 years, starting with the Reduced Enrichment for...
Ferritic/martensitic steels, particularly those modified to include nanoscale oxide dispersions, show great promise for use in structural...

Advancement in energy, environment and biology research relies heavily on micro-, nano- and atomic-scale chemical and structural imaging. Many microscopy instruments have high-resolution imaging capabilities including complementary chemical, structural and phase information, in-situ imaging in native environments and imaging of dynamic processes with high temporal-resolution. See a complete list of Microscopy instruments.

Resources and Techniques

  • Nanoscale and sub-nanoscale imaging allows users to elucidate chemical processes and acquire structural data for a variety of samples such as nanostructures and cell-surface proteins.
  • Tomography yields three-dimensional reconstruction of transmission electron microscopy images generated for biological samples as well as for soft materials and samples with 3D structural heterogeneity.
  • Environmental particle analysis offers knowledge about non-volatile atmospheric particle composition and hydration properties using high-pressure scanning electron microscopy equipped with energy-dispersive x-ray analysis capability.
  • Environmental mode imaging techniques enable sample preservation to eliminate extensive preparation procedures that can introduce artifacts and make possible live-cell imaging and in situ imaging in liquids or controlled gas environments with high resolution microscopy.
  • Dynamic imaging capability enables real-time studies of nanosecond-scale dynamic processes with unprecedented spatial resolution, such as protein-protein interactions, with contrast at the single-molecule level.

Quiet Wing for Advanced Microscopy
Seven microscopes are housed in the Quiet Wing, a space specially designed to reduce external factors, such as vibrations and electromagnetic fields, that can impede capture of high-resolution images. Read more about the Quiet Wing and its instrumentation.

A new DTEM – Dynamic Transmission Electron Microscope – is under development at EMSL in collaboration with scientific colleagues at Pacific Northwest National Laboratory. It will be housed in the Quiet Wing. To learn more about this system, the science it will advance and its historical development, visit the DTEM page.

Pages

Leads

(509) 371-6233

Dr. Lea has over 20 years of experience with research related to surface science. His primary focus areas are related to the application of electron spectroscopy and scanning probes to study chemical and geochemical processes, biomolecular...