NMR and EPR
EMSL houses nuclear magnetic resonance (NMR) instruments with frequencies up to 900 MHz, as well as an electron paramagnetic resonance (EPR) spectrometer. EMSL staff are active in developing a variety of probes and techniques to complement their collection of state-of-the-art magnets. NMR capabilities at EMSL are widely used in the following topical areas:
- Interfacial and in situ chemistry—EMSL offers users solid-state NMR techniques for low-gamma nuclei detection and a full range of magic angle spinning methodologies to study catalytic, advanced material, geochemical, and biological systems in their near-native state and in real time.
- Interfacial and in situ biology—NMR resources are available for structural biology investigations to determine the structure of large molecular assemblies and to monitor their changes in response to environmental influences. In addition, EMSL offers tools for functional omics research, including metabolomics, and a bio-magic angle spinning (BioMAS)/900-MHz NMR system to explore biological membrane proteins in the solid state.
- Environmental chemistry—EMSL offers a unique NMR system for radiological studies. Users can perform magic angle spinning of highly radioactive samples with a novel triple-containment rotor. These tools allow users to apply NMR techniques to critical areas of radiological research, including the study of radioactive waste processing and storage.
- Metallocomplexes—EMSL emphasizes studies of transition metal complexes relevant to biological, environmental, and catalysis systems. BioMAS and low-temperature probes are used to determine conformational changes in membrane protein complexes involving metal clusters as followed by solid-state NMR and pulsed EPR.
Refer to the table below for a full listing, which leads to complete information about each of EMSL's NMR and EPR instruments. Brief details about the primary NMR and EPR tools available to EMSL users immediately follow.
- NMR spectrometers, ranging from 300 MHz to 900 MHz with capabilities for high-field liquid-state, solid-state, and micro-imaging techniques
- Pulsed EPR spectrometry capable of probing metal centers in biological and materials systems
- NMR with radiological capabilities
- NMR with metabolomics capabilities to study microbial systems and biofluids
- Extreme-temperature probes, including high-temperature probes to study catalysts in their near-native states and low-temperature probes to study metalloprotein chemistry and structure
- Novel BioMAS/900-MHz system for biosolid studies
- Combined optical and magnetic resonance microscopy
- Virtual NMR tools for remote access to spectrometer systems
All Related Publications Related Publications
- Identification of Fragile Microscopic Structures during Mineral Transformations in Wet Supercritical CO2.
- Forsterite [Mg2SiO4)] Carbonation in Wet Supercritical CO2: An in situ High Pressure X-Ray Diffraction Study.
- Making Li-air batteries rechargeable: material challenges.
- Experimental study of crossover from capillary to viscous fingering for supercritical CO2 - water displacement in a homogeneous pore network.
- Mixed-Isotope Labeling with LC-IMS-MS for Characterization of Protein-Protein Interactions by Chemical Cross-Linking .
All Related Research Highlights Related Research Highlights
- Biofilms move electrons long distances across two distinct layers, even under starving conditions (Long distance)
- EMSL tools reveal morphology, growth mechanisms of precipitates from scCO2 storage (Rods and rosettes)
- Biochemical studies provide insight into an RNA silencing pathway (The great repression)
- Protein signaling between soybean root hairs, bacteria reveals core cellular processes (Orchestrating change)
- Using an integrated ‘omics approach to study macrophages (Activating macrophages)