Mammalian Cell Culture
At EMSL, researchers use the single-molecule fluorescence/patch clamp microscope to combine high-sensitivity fluorescence imaging simultaneously with physiological measurements to identify real-time molecular interactions and conformational changes of cell-membrane receptors and their functional consequences.
The study of individual proteins in the membrane of living cells is enabled by the presence of a tissue culture facility adjacent to the microscopes. The facility supports the growth of eukaryotic primary cells or cell-lines, including human cell lines. In addition to a bio-hood, the facility includes CO2 incubators, a centrifuge, 37°C water bath and a shaker.
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- A multi-omic systems approach to elucidating Yersinia virulence mechanisms.
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- Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation.
- Cellular Recognition and Trafficking of Amorphous Silica Nanoparticles by Macrophage Scavenger Receptor A.
- Controlling the Response: Predictive Modeling of a Highly Central, Pathogen-Targeted Core Response Module in Macrophage Activation.
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