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Spectrometer: Stopped-Flow, Absorbance, BioLOGIC SFM-400

Quick Specs

  • Four-syringe system
  • Minimum deadtime of 0.25 ms
  • Sample volume less than 0.03 mL
  • Able to record kinetic data in 50μs increments

The Bio-Logic® SFM-400/S is a 4-syringe stopped-flow system that offers the capability to carry out complex, multi-mixing experiments with the lowest rates of aging and deadtimes. The 4-syringe system offers a minimum deadtime of 0.25 ms with a microcuvette. In tests conducted in EMSL using regular cuvettes, an instrument deadtime of less than 1 ms with the FC08 (0.8-mm light pathlength) or ~ 2 ms with the FC10/100 (10-mm light pathlength) was easily achieved. The small sample volume of less than 0.03 ml makes the system ideal for experiments such as those involving biological reactants for which minimum consumption of reagents is desired. The availability of four syringes facilitates sophisticated experiments that require sample pre-mix and timed aging. The fast sampling rate of the MOS-250 spectrometer allowed its users to record kinetic data in time increments as little as 50 microseconds.

All Related Publications Related Publications

  1. The surface structure of α-uranophane and its interaction with Eu(III) – An integrated computational and fluorescence spectroscopy study.
  2. Direct Numerical Simulation of Pore-Scale Flow in a Bead Pack: Comparison with Magnetic Resonance Imaging Observations.
  3. Electron Exchange and Conduction in Nontronite from First-Principles.
  4. Rapid electron exchange between surface-exposed bacterial cytochromes and Fe(III) minerals.
  5. Oxide Melt Solution Calorimetry of Fe2+ -bearing Oxides and Application to the Magnetite - Maghemite (Fe3O4-Fe8/3O4) system.

Related Research Highlights

  1. EMSL’s Chinook provides a new angle for validating pore-scale flow simulations (Go with the flow)
  2. New finding shows a research area to expand in EMSL Radiochemistry Annex (Promising Science for Plutonium Cleanup )
  3. Shewanella proteins could be used to generate energy or immobilize contaminants (Wired Microbe Conducts Electricity)
  4. Insights on underappreciated reaction could shed light on environmental cleanup options (Unexpected, Stable Form of Uranium Detected)
Wang, Zheming | , 509-371-6349