Mass Spectrometer: Ion Mobility Spectrometry, Time of Flight
Four integration separation platforms are being built, combining liquid chromatography (LC), ion mobility spectrometry (IMS), and TOF mass spectrometry for increased throughput and sensitivity in proteomics, metabolomics, lipidomics, and others. This will enable higher throughput analysis of biological systems. Each separation technology, LC, IMS, and TOF-MS, will be optimized for analysis resolution, sensitivity, and speed. Initial application of the LC-IMS-TOF capability includes analysis of a large (~3000) cohort of archived serum samples to discover biomarkers for predicting of age-related loss of muscle mass (sarcopenia). Besides yielding blood protein biomarkers predicting the onset and severity of sarcopenia development, this study will be the first demonstration of a large, population-size effort for discovery of clinical biomarkers. Such an effort is an important step toward personalized medicine as the cohort size is a highly crucial parameter to achieve necessary statistical power for biomarker discovery given the inherent diversity of the human population.
All Related Publications Related Publications
- STEPS: A Grid Search Methodology for Optimized Peptide Identification Filtering of MS/MS Database Search Results.
- Mixed-Isotope Labeling with LC-IMS-MS for Characterization of Protein-Protein Interactions by Chemical Cross-Linking .
- High-Definition Differential Ion Mobility Spectrometry with Resolving Power up to 500.
- Identification of widespread adenosine nucleotide binding in Mycobacterium tuberculosis.
- The Role of a Dipeptide Outer-Coordination Sphere on H2 -Production Catalysts: Influence on Catalytic Rates and Electron Transfer.
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