Mass Spectrometry

Systems biology and complex mixture studies in biofuels, microbial communities, climate and environmental remediation can be analyzed with word-class separations and mass spectrometry capabilities. See a complete list of Mass Spectrometry instruments.

Resources and Techniques
Panomics - Advanced global proteomics, metabolomics, glycomics and activity-based omics research using cutting-edge tools, including customized hardware and sophisticated bioinformatics tools. This research includes:

  • Confident protein identification and quantitation using stable-isotope labeling and label-free strategies
  • Subcellular localization, turnover rates and modification states of proteins
  • Top-down proteomics and broad intact-protein level measurements
  • Characterization of protein-protein/metabolite interactions
  • Activity based proteomics and other targeted proteomics strategies such as phosphoproteomics and biomarker validation.

Natural Organic Matter - Several workflows targeting different classes of organic compounds in soil and the environment have been developed.

Aerosol Particle Characterization - Real-time data is captured on environmentally relevant aerosols with high specificity and resolution using field-deployable equipment.

Ion-surface Collision - Fundamental aspects of activation, dissociation and deposition (soft-landing) of complex molecular ions are studied following collision with specially prepared surfaces using uniquely configured instrumentation.

A new state-of-the-art 21T FTICR mass spectrometer will be online this summer. To learn more about this system, the science it will advance, and its historical development, visit the 21T HRMAC page.

Other research resources found in EMSL and managed by Pacific Northwest National Laboratory include:

Description

Proteomics Capabilities

  • High resolution and mass accuracy Fourier-transform ion cyclotron resonance (FT-ICR) spectrometers, from 6 Tesla (T) to 15T and 21T in development
  • Orbitrap based platforms including Elite, Velos and Exactive mass spectrometers
  • Triple-quadrupole mass spectrometers for targeted quantitation
  • Gas Chromatography (GC) MS instruments with extensive compound identification libraries
  • Ion mobility spectrometry (IMS) coupled to time-of-flight (TOF) mass spectrometers
  • Advanced custom nano-HPLC systems, augmented by Agilent, Waters and Eksigent systems
  • MALDI and C60 SIMS mass spectrometry imaging (MSI) ion sources

 Aerosol Capabilities

  • LTQ-Orbitrap
  • Field-deployable, second-generation, single-particle, laser-ablation, TOF mass spectrometer (SPLAT II)
  • Proton transfer reaction mass spectrometer
  • High-resolution TOF aerosol mass spectrometer

Ion-Surface Collision Study Capabilities

  • 6T FT-ICR spectrometer configured for studying ion-surface interactions
  • Ion deposition instrument for preparation of novel materials using ion soft-landing
  • TOF secondary ion mass spectrometer (TOF-SIMS)

 

Instruments

The 6-Tesla High-Field Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FT-ICR MS), is a unique instrument designed and constructed at...
Custodian(s): Julia Laskin
The Neptune is a mid-resolution, multi-collector ICP-MS instrument capable of simultaneous collection of up to nine elemental/isotopic masses,...
Custodian(s): M Lizabeth Alexander
Nano-DESI/HRMS analytical platform allows in-depth molecular characterization of very small samples of organic materials  (down to 10 ng) and...
Custodian(s): Alexander Laskin
The Agilent 4500 Series inductively coupled plasma mass spectrometer (ICP-MS) is available for all research areas requiring analysis of trace metals...
Custodian(s): Tom Wietsma
The Quadrupole Aerosol Mass Spectrometer (QAMS) manufactured by Aerodyne Inc., was added as a capability in the EMSL user facility in 2004 and the...
Custodian(s): M Lizabeth Alexander

Publications

Dual beam depth profiling strategy has been widely adopted in ToF-SIMS depth profiling, in which two basic operation modes, interlaced mode and non-...
The ability of Leishmania to survive in their insect or mammalian host is dependent upon an ability to sense and adapt to changes in the...
Fusions between the transmembrane protease serine 2 (TMPRSS2) and ETS related gene (ERG) represent one of the most specific biomarkers that define a...
Grain growth of nanocrystalline materials is generally thermally activated, but can also be driven by irradiation at much lower temperature. In...
14YWT oxide dispersion strengthened (ODS) ferritic steel was irradiated with of 5 MeV Ni2+ ions, at 300 °C, 450 °C, and 600 °...

Science Highlights

Posted: July 29, 2015
Rice is a major food source for a majority of the world's population, but it's also a significant source of manmade atmospheric methane, a potent...
Posted: July 13, 2015
Researchers can now see biofilms, structured communities of microbes, in 3-D and real time. These images give scientists insight into the role...
Posted: May 26, 2015
The Science Soils contain a vast amount of carbon and are responsible for most greenhouse gas emissions. However, current models do not predict...
Posted: May 15, 2015
For 20 years, models explained only a small fraction of the carbon-rich secondary organic aerosols, or SOA, measured in the air. Due to a lack of...
Posted: February 20, 2015
The Science Secondary organic aerosol (SOA) particles represent a major component of atmospheric particulates and are known to affect climate, air...

Instruments

The interaction between plants and their environment is of key importance in promoting sustainable, healthy, and robust plant growth. Underlying the...
The microbial activities on which humanity and ecosystems depend are determined by microbial community membership and by the resources and conditions...
Enzymatic hydrolysis of (ligno)cellulose by cellulases is key for the production of second generation biofuels which represent a long-standing...
While nitrogen management is an important agronomic trait in any cropping system, it is of particular concern for future bioenergy crops that will be...
The proposed project will advance understanding of the response of methane production and methane oxidation to changes in plant productivity so that...

Systems biology and complex mixture studies in biofuels, microbial communities, climate and environmental remediation can be analyzed with word-class separations and mass spectrometry capabilities. See a complete list of Mass Spectrometry instruments.

Resources and Techniques
Panomics - Advanced global proteomics, metabolomics, glycomics and activity-based omics research using cutting-edge tools, including customized hardware and sophisticated bioinformatics tools. This research includes:

  • Confident protein identification and quantitation using stable-isotope labeling and label-free strategies
  • Subcellular localization, turnover rates and modification states of proteins
  • Top-down proteomics and broad intact-protein level measurements
  • Characterization of protein-protein/metabolite interactions
  • Activity based proteomics and other targeted proteomics strategies such as phosphoproteomics and biomarker validation.

Natural Organic Matter - Several workflows targeting different classes of organic compounds in soil and the environment have been developed.

Aerosol Particle Characterization - Real-time data is captured on environmentally relevant aerosols with high specificity and resolution using field-deployable equipment.

Ion-surface Collision - Fundamental aspects of activation, dissociation and deposition (soft-landing) of complex molecular ions are studied following collision with specially prepared surfaces using uniquely configured instrumentation.

A new state-of-the-art 21T FTICR mass spectrometer will be online this summer. To learn more about this system, the science it will advance, and its historical development, visit the 21T HRMAC page.

Other research resources found in EMSL and managed by Pacific Northwest National Laboratory include:

Pages

Leads

(509) 371-6341

As part of the EMSL mission, his major projects include implementing the next generation state-of-the-art mass spectrometry instrumentation for biological systems analysis such as the recently completed EMSL capital investment projects funded by...