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John Cort

Institution
Pacific Northwest National Laboratory
ORCID
ORCID logo 0000-0001-6342-6461

Projects

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

Pentosan Polysulfate Profiling with NMR Spectroscopy

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
The proposed research will apply a heteronuclear nuclear magnetic resonance (NMR) spectroscopy method for profiling the molecular composition of pentosan polysulfate (PPS) in its pure active…

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

The Lignin Enigma: Turning the Corner

Lead Institution
Washington State University
Principal Investigator
Norman Lewis
Project type
Large-Scale EMSL Research
Lignins (Nature's second most abundant terrestrial plant substances) provide structural support for plants to stand upright, to conduct water/nutrients, and as barriers to opportunistic…

GC-MS Analysis of Alkaline Cellulose Degradation Products

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
Project type
Exploratory Research
Degradation of cellulose and other polysaccharides in alkaline conditions and at elevated temperatures yields mixtures of compounds containing carboxylic acid and/or alcohol functional groups. These…

NMR profiling of monoclonal antibodies

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
High resolution solution state NMR spectroscopy will be used to profile monoclonal antibodies. Two antibodies will be studied, 1) a candidate NIST standard Fab domain, and 2) trastuzumab, an FDA…

Dirigent Protein Structure and Mechanism

Lead Institution
Washington State University
Principal Investigator
Norman Lewis
Project type
Large-Scale EMSL Research
The biochemistry of vascular plants has evolved in such a way as to rely extensively upon unique phenoxy radical coupling reactions thereby generating substances for structural support, for formation…

Structural Genomics Collaborative Access Team (CAT)

Lead Institution
Miami University
Principal Investigator
Michael Kennedy
The Structural Genomics CAT will carry out research focused in structural genomics and related research areas with a primary goal of increasing throughput, maximizing efficiency of existing resources…

NMR-based metabolomics of the enteropathogens Salmonella and Yersinia

Lead Institution
Washington State University Tri-Cities
Principal Investigator
Kathleen McAteer
Project type
Large-Scale EMSL Research
The goal of this research is to analyze and reconstruct intercellular and intracellular networks in Salmonella and Yersinia using multiple types of -omics data. Although we hope to gain a more…

Quantitative Characterization of Biomass Deconstruction

Lead Institution
Washington State University Tri-Cities
Principal Investigator
Bin Yang
Project type
Exploratory Research
The objective of this research is to apply advanced characterization techniques to gain a deeper understanding of lignocellulosic biomass conversion to biofuels. The proposed research will take…

Protein Interactions and Interfaces

Lead Institution
Rutgers University
Principal Investigator
Gaetano Montelione
Project type
Large-Scale EMSL Research
All proteins interact with other molecules: ligands, metals, membranes, surfaces or other proteins. Such interactions are intrinsic to protein function. We propose to leverage our successful program…

Proteomic Assessment of Genes of Unknown Function

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Joshua Adkins
Understanding the full repertoire of biological functions has proven elusive, hampering our ability to predict outcomes of biological processes, such as infections or impacts of toxins. PNNL will…

Structure of Allochromatium vinosum DsrC protein

Lead Institution
Los Alamos National Laboratory
Principal Investigator
Thomas Terwilliger
Project type
Capability Research
Project Description: The dsrC gene codes for the 12.7 kDa protein DsrC, also known as the gamma subunit of dissimilatory sulfite reductase, and occurs in all sulfate-reducing bacteria (e.g…

Discovery of Host Proteins Involved in Host-Pathogen Interactions

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Joshua Adkins
The multiple mechanisms by which pathogenic enterobacteria of the genus Salmonella infect their host indicate a complex host-pathogen relationship. The pathogen secretes dozens of proteins, termed &…

Proton and Carbon Chemical Shift Assignments of Brodifacoum

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
Project type
Limited Scope
We are making complete proton and carbon chemical shift assignments of both diastereomers of the potent vitamin K epoxide reductase (VKOR) inhibitor brodifacoum ((3-[3-[4-(4-bromophenyl)phenyl]-1,2,3…

Signature Discovery in Organic Compounds

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
This project will survey physical and chemical characteristics of organic compounds of interest in chemical forensics applications.The goal is to develop chemical attribution signatures (CAS’s) for…

Evaluating the role of DsrC as a key protein in dissimilatory sulfur metabolism

Lead Institution
Instituto de Tecnologia Quimica e Biologica
Principal Investigator
Ines Pereira
Project type
Large-Scale EMSL Research
The dissimilatory reduction of sulfur compounds is one of the earliest energy metabolisms detected on Earth, at ~3.5 billion years ago. Sulfate reducing bacteria (SRB) are prominent among sulfur…

Proteomic Assessment of Genes of Unknown Function

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Joshua Adkins
Understanding the full repertoire of biological functions has proven elusive, hampering our ability to predict outcomes of biological processes, such as infections or impacts of toxins. PNNL will…

High-Throughput Structure Determination of Proteins and Protein Complexes

Lead Institution
Rutgers University
Principal Investigator
Gaetano Montelione
Project type
Large-Scale EMSL Research
The Northeast Structural Genomics Consortium (NESGC, www.nesg.org) is one of four large protein structure production centers supported by the NIH Protein Structure Initiative II (the PSI II). The…

Discovery of Host Proteins Involved in Host-Pathogen Interactions

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Joshua Adkins
The multiple mechanisms by which pathogenic enterobacteria of the genus Salmonella infect their host indicate a complex host-pathogen relationship. The pathogen secretes dozens of proteins, termed &…

1H AND 13C NMR STUDY OF BRODIFACOUM STEREOCHEMISTRY AND STRUCTURE

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
Project type
Exploratory Research
Brodifacoum is a lipid-soluble warfarin derivative that inhibits vitamin K epoxide reductase (VKOR) and exhibits potent anticoagulant activity. Because of its chemical stability and use in numerous…

Signature Discovery in Organic Compounds

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
This project will survey physical and chemical characteristics of organic compounds of interest in chemical forensics applications.The goal is to develop chemical attribution signatures (CAS’s) for…

A Systems Biology Approach to Infectious Diseases Research

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Joshua Adkins
Project type
Exploratory Research
We describe an integrated program for coupling advanced capabilities in high-throughput transcriptomics, proteomics, and metabolomics with a comprehensive informatics infrastructure and a…

Study of temperature-sensitive mutants of yeast guanylate kinase

Lead Institution
Washington State University
Principal Investigator
Ping Ye
Project type
Limited Scope
The ability to switch genes on and off spatially and temporally is important for understanding individual gene function in complex biological processes. Despite the fact that a variety of conditional…

Deciphering Microbial Communication Through Metabolites

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Thomas Metz
This project will establish a Metabolomics Center at PNNL that will develop the advanced measurement capabilities necessary to define the metabolic processes of microbiomes and determine how these…

Undiagnosed Diseases Network Metabolomics Core

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Thomas Metz
A state of the art metabolomics core will be a vital part of the Undiagnosed Diseases Network (UDN). However, in order for the UDN to realize it’s the full potential it is essential that the…

Pentosan Polysulfate Profiling with NMR Spectroscopy

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
The proposed research will apply a heteronuclear nuclear magnetic resonance (NMR) spectroscopy method for profiling the molecular composition of pentosan polysulfate (PPS) in its pure active…

A Systems Biology Approach to Infectious Diseases Research

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Joshua Adkins
Project type
Exploratory Research
We describe an integrated program for coupling advanced capabilities in high-throughput transcriptomics, proteomics, and metabolomics with a comprehensive informatics infrastructure and a…

Structure of the binding interface between leiomodin and tropomyosin.

Lead Institution
Washington State University
Principal Investigator
Alla Kostyukova
Project type
Limited Scope
Cardiac muscle cells contain two types of protein filaments composed of either actin or myosin. The filaments slide past one another and produce force in the heart muscle. For healthy heart…

PNNL/NESG Structural Genomics

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
Project type
Capability Research
PNNL is a member of the Northeast Structural Genomics Consortium (NESG), a NIH-funded pilot project intended to explore the feasability of and technical challenges presented by high-throughput…