The project aims to develop a high throughput and multiplex affinity purification mass spectrometry (AP-MS) assay by seamless integration of protein microarray and nanoscale proteomics. We will immobilize SARS-COV-2 proteins on a glass slide by printing them to an array of micro-spots. Then, a cell lysate is applied to the SARS-COV-2 protein microarray chip and incubate to allow multiplex affinity purification. To identify and quantify the captured human proteins on the protein array, nanoPOTS (nanodroplet processing in one-pot for trace samples) will be used to digest the captured proteins followed by LC-MS analysis.
COVID-19: High-throughput SARS-CoV-2-Human protein-protein interactome and drug discovery on a nanowell array chip
Thursday, July 9, 2020
Environmental Molecular Sciences Laboratory
Wednesday, September 30, 2020