Environmental Molecular Sciences Laboratory

A DOE Office of Science User Facility

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COVID-19: High-throughput SARS-CoV-2-Human protein-protein interactome and drug discovery on a nanowell array chip

Date: 
Thursday, July 9, 2020
Principal Investigator: 
Ying Zhu
Lead Institution: 
Environmental Molecular Sciences Laboratory
Closed Date: 
Wednesday, September 30, 2020
Project ID: 
51584
Abstract: 

The project aims to develop a high throughput and multiplex affinity purification mass spectrometry (AP-MS) assay by seamless integration of protein microarray and nanoscale proteomics. We will immobilize SARS-COV-2 proteins on a glass slide by printing them to an array of micro-spots. Then, a cell lysate is applied to the SARS-COV-2 protein microarray chip and incubate to allow multiplex affinity purification. To identify and quantify the captured human proteins on the protein array, nanoPOTS (nanodroplet processing in one-pot for trace samples) will be used to digest the captured proteins followed by LC-MS analysis.