Mass Spectrometry

Systems biology and complex mixture studies in biofuels, microbial communities, climate and environmental remediation can be analyzed with world-class separations and mass spectrometry capabilities. See a complete list of Mass Spectrometry instruments.

Resources and Techniques
Panomics - Advanced global proteomics, metabolomics, glycomics and activity-based omics research using cutting-edge tools, including customized hardware and sophisticated bioinformatics tools. This research includes:

  • Confident protein identification and quantitation using stable-isotope labeling and label-free strategies
  • Subcellular localization, turnover rates and modification states of proteins
  • Top-down proteomics and broad intact-protein level measurements
  • Characterization of protein-protein/metabolite interactions
  • Activity based proteomics and other targeted proteomics strategies such as phosphoproteomics and biomarker validation.

Natural Organic Matter - Several workflows targeting different classes of organic compounds in soil and the environment have been developed.

Aerosol Particle Characterization - Real-time data is captured on environmentally relevant aerosols with high specificity and resolution using field-deployable equipment.

Ion-surface Collision - Fundamental aspects of activation, dissociation and deposition (soft-landing) of complex molecular ions are studied following collision with specially prepared surfaces using uniquely configured instrumentation.

A new state-of-the-art 21T FTICR mass spectrometer will be online this summer. To learn more about this system, the science it will advance, and its historical development, visit the 21T HRMAC webpage.

Other research resources found in EMSL and managed by Pacific Northwest National Laboratory include:

Description

Proteomics Capabilities

  • High resolution and mass accuracy Fourier-transform ion cyclotron resonance (FT-ICR) spectrometers, from 6 Tesla (T) to 15T and 21T in development
  • Orbitrap based platforms including Elite, Velos and Exactive mass spectrometers
  • Triple-quadrupole mass spectrometers for targeted quantitation
  • Gas Chromatography (GC) MS instruments with extensive compound identification libraries
  • Ion mobility spectrometry (IMS) coupled to time-of-flight (TOF) mass spectrometers
  • Advanced custom nano-HPLC systems, augmented by Agilent, Waters and Eksigent systems
  • MALDI and C60 SIMS mass spectrometry imaging (MSI) ion sources

 Aerosol Capabilities

  • LTQ-Orbitrap
  • Field-deployable, second-generation, single-particle, laser-ablation, TOF mass spectrometer (SPLAT II)
  • Proton transfer reaction mass spectrometer
  • High-resolution TOF aerosol mass spectrometer

Ion-Surface Collision Study Capabilities

  • 6T FT-ICR spectrometer configured for studying ion-surface interactions
  • Ion deposition instrument for preparation of novel materials using ion soft-landing
  • TOF secondary ion mass spectrometer (TOF-SIMS)

 

Instruments

More details about this instrument willl be available soon.
Custodian(s): Tom Wietsma
The Agilent 4500 Series inductively coupled plasma mass spectrometer (ICP-MS) is available for all research areas requiring analysis of trace metals...
Custodian(s): Tom Wietsma
EMSL’s 21 Tesla Fourier transform ion cyclotron resonance mass spectrometer, or 21T FTICR MS, is one of only two in the world with this high of a...
Custodian(s): Ljiljana Pasa-Tolic
The Quadrupole Aerosol Mass Spectrometer (QAMS) manufactured by Aerodyne Inc., was added as a capability in the EMSL user facility in 2004 and the...
Custodian(s): M Lizabeth Alexander
Type of Instrument:
Mass Spectrometer
More details on this  instrument will be available soon.
Custodian(s): Ljiljana Pasa-Tolic

Publications

Methacryoyl peroxynitrate (MPAN), the acylperoxyl nitrate of methacrolein, has been suggested to be an important secondary organic aerosol (SOA)...
We report the first evaluation of a platform coupling a high speed field asymmetric ion mobility spectrometry microchip (µFAIMS) with drift...
We report the results of a VAMAS (Versailles Project on Advanced Materials and Standards) interlaboratory study on the measurement of composition in...
Freshly emitted soot particles are fractal-like aggregates, but atmospheric processing often transforms their morphology. Morphology of soot...
Herein, we employ a combination of reduction synthesis in solution, soft landing of mass-selected precursor and product ions, and in situ time-of-...

Science Highlights

Posted: March 20, 2017
The Science Molds produce a wide range of both valuable and toxic molecules, which have important implications for energy production, agriculture...
Posted: March 09, 2017
The Science The abundance of woody deciduous shrubs is increasing in the Arctic and this changing vegetative landscape could profoundly impact the...
Posted: February 07, 2017
Turning electricity from large-scale solar and wind power projects into fuel would be useful for storing the energy until needed. The challenge is...
Posted: February 01, 2017
The Science Vitamin B12 regulates the production of DNA and many proteins required for normal cellular function. A recent study revealed this...
Posted: January 23, 2017
Soil contains networks of pores and channels composed of different minerals and formed by roots, insects and other organisms. The pores hold liquids...

Instruments

The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our...
Enzymatic hydrolysis of (ligno)cellulose by cellulases is key for the production of second generation biofuels which represent a long-standing...
The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our...
Atmospheric aerosols have a profound effect on climate by scattering and absorbing solar and terrestrial radiation. They can be predominantly...
Atmospheric aerosols affect the climate directly by absorbing and scattering radiation and indirectly by impacting the properties and lifetimes of...

Systems biology and complex mixture studies in biofuels, microbial communities, climate and environmental remediation can be analyzed with world-class separations and mass spectrometry capabilities. See a complete list of Mass Spectrometry instruments.

Resources and Techniques
Panomics - Advanced global proteomics, metabolomics, glycomics and activity-based omics research using cutting-edge tools, including customized hardware and sophisticated bioinformatics tools. This research includes:

  • Confident protein identification and quantitation using stable-isotope labeling and label-free strategies
  • Subcellular localization, turnover rates and modification states of proteins
  • Top-down proteomics and broad intact-protein level measurements
  • Characterization of protein-protein/metabolite interactions
  • Activity based proteomics and other targeted proteomics strategies such as phosphoproteomics and biomarker validation.

Natural Organic Matter - Several workflows targeting different classes of organic compounds in soil and the environment have been developed.

Aerosol Particle Characterization - Real-time data is captured on environmentally relevant aerosols with high specificity and resolution using field-deployable equipment.

Ion-surface Collision - Fundamental aspects of activation, dissociation and deposition (soft-landing) of complex molecular ions are studied following collision with specially prepared surfaces using uniquely configured instrumentation.

A new state-of-the-art 21T FTICR mass spectrometer will be online this summer. To learn more about this system, the science it will advance, and its historical development, visit the 21T HRMAC webpage.

Other research resources found in EMSL and managed by Pacific Northwest National Laboratory include:

Pages

Leads

Dr. Lipton is EMSL's mass spectrometry capability lead. She is also a senior staff scientist at Pacific Northwest National Laboratory.

She is nationally recognized for her research in microbial proteomics, specifically for developing and...