Environmental Molecular Sciences Laboratory

A DOE Office of Science User Facility

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Plant, Soil and Subsurface Flow and Transport

The Plant, Soil, & Subsurface Flow and Transport Capability comprises a suite of resources for exploring physical and biogeochemical processes in the subsurface, and in the plant-soil ecosystem. Researchers can conduct experiments across a range of scales, and compare experimental and numerical results to address the nation's most challenging problems related to contaminant transport, biogeochemical nutrient cycles, plant physiology, and plant-microbe-ecosystem interactions.

The resources include:

  • A plant growth laboratory with shared resources between PNNL and EMSL, where light, temperature, watering conditions, and CO2 concentration can be carefully controlled.
  • Microbial ecology laboratories for incubating cultures and communities under ambient or increased temperature/CO2 levels, and measuring their respiration and enzyme capacity.
  • Sample preparation for metatranscriptomics, metagenomics, high throughput amplicon sequencing, and other biogeochemical assays.
  • Custom flow cells at the pore- and intermediate- scales to study reactive and multiphase flow in saturated and undersaturated subsurface conditions.
  • Simulation of multiphase flow to design experiments, provide initial and boundary conditions, and to aid interpretation of results.
  • X-ray Computed Tomography for 3-dimensional imaging of the internal structure of environmental and other samples.
  • An analytical laboratory for C, H, N, S and inorganic assays.

Of course EMSL’s extensive range of instrumentation in other capabilities is available for further detailed analysis, including isolation of distinct cells and tissues, microscopy (e.g. fluorescence, electron, and atomic-force) as well as multiple -omic analyses.

See a complete list of instruments and laboratories available in the Plant, Soil & Subsurface Flow and Transport capability.

Description

Capability Details

  • Intermediate-scale columns and flow cells
  • Pore-scale microfluidic and imaging capabilities
  • High-pressure/temperature cells for research at reservoir conditions
  • Phytotron
  • Molecular Ecology Laboratory
  • Soil Incubation Laboratory
  • Dual energy gamma radiation system
  • Integrated hydraulic properties apparatus
  • Relative permeability apparatus
  • Analytical tools including ion, liquid and gas chromatographs, inductively coupled plasma-mass spectrometry and carbon analysis tools

Instruments

EMSL housess two instruments for measuring the quantities of Carbon, Nitrogen, Hydrogen, Sulfur, and Oxygen in solid or liquid samples: A CHNS/O...
Custodian(s): Tom Wietsma
Researchers in the Intermediate Flow Cell laboratory. A custom cell with multiple sampling ports can be seen at left, positioned for dual-gamma...
Custodian(s): Tom Wietsma
More details available soon.
Custodian(s): Kirsten Hofmockel
EMSL's Plant Sciences Laboratory, an enclosed research greenhouse used for studying interactions between plants and the environment, provides walk-in...
Custodian(s): Christer Jansson
The pore-scale micromodel flow and transport lab is part of EMSL's Subsurface Flow and Transport Laboratory (SFTL) with a focus on coupled (...
Custodian(s): Tom Wietsma

Publications

The wettability of silicon and glass surfaces can be modified by silanization. However, similar treatments of glass and silica surfaces using the...
A series of displacement experiments was conducted using five wetting-nonwetting immiscible fluid pairs in a homogenous and uniform pore network. The...
The objectives of this work were to determine if a porescale model could accurately capture the physical and chemical processes that control...
Microbial degradation of contaminants in the subsurface requires the availability of nutrients; this is impacted by porous media heterogeneity and...
The use of air-water, ?wa, or air-liquid contact angles is customary in surface science, while oil-water contact angles ?ow, are of paramount...

Science Highlights

Posted: July 15, 2020
  A team of Pacific Northwest National Laboratory (PNNL) and EMSL scientists have compiled the most comprehensive genetic-level data set of the...
Posted: April 23, 2020
Microbial communities are vital to the health of soil ecosystems by performing critical functions like carbon and nutrient cycling. To better...
Posted: October 31, 2019
The Science Drought has plagued agriculture for centuries. Scientists suspect the reason plants survive under drought may have something to do with...
Posted: November 30, 2016
Researchers at the Department of Energy's Pacific Northwest National Laboratory injected carbon dioxide, or CO2, into basalt lava flows a half-mile...
Posted: June 03, 2016
Geochemists at Pacific Northwest National Laboratory used EMSL capabilities to help developed a mathematical formula to scale subsurface reaction...

Instruments

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a...
The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a...
Remediation of metal and radionuclide contaminants in soil and groundwater systems is challenging because of their strong chemical interactions with...

The Plant, Soil, & Subsurface Flow and Transport Capability comprises a suite of resources for exploring physical and biogeochemical processes in the subsurface, and in the plant-soil ecosystem. Researchers can conduct experiments across a range of scales, and compare experimental and numerical results to address the nation's most challenging problems related to contaminant transport, biogeochemical nutrient cycles, plant physiology, and plant-microbe-ecosystem interactions.

The resources include:

  • A plant growth laboratory with shared resources between PNNL and EMSL, where light, temperature, watering conditions, and CO2 concentration can be carefully controlled.
  • Microbial ecology laboratories for incubating cultures and communities under ambient or increased temperature/CO2 levels, and measuring their respiration and enzyme capacity.
  • Sample preparation for metatranscriptomics, metagenomics, high throughput amplicon sequencing, and other biogeochemical assays.
  • Custom flow cells at the pore- and intermediate- scales to study reactive and multiphase flow in saturated and undersaturated subsurface conditions.
  • Simulation of multiphase flow to design experiments, provide initial and boundary conditions, and to aid interpretation of results.
  • X-ray Computed Tomography for 3-dimensional imaging of the internal structure of environmental and other samples.
  • An analytical laboratory for C, H, N, S and inorganic assays.

Of course EMSL’s extensive range of instrumentation in other capabilities is available for further detailed analysis, including isolation of distinct cells and tissues, microscopy (e.g. fluorescence, electron, and atomic-force) as well as multiple -omic analyses.

See a complete list of instruments and laboratories available in the Plant, Soil & Subsurface Flow and Transport capability.

Pages

Leads

Dr. Bowden manages EMSL's optical spectroscopy and diffraction, subsurface flow and transport, and microfabrication and deposition capabilities. He is responsible for the X-ray diffraction facility and assists or conducts measurement and analysis...