Environmental Molecular Sciences Laboratory

A DOE Office of Science User Facility

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Plant, Soil and Subsurface Flow and Transport

The Plant, Soil, & Subsurface Flow and Transport Capability comprises a suite of resources for exploring physical and biogeochemical processes in the subsurface, and in the plant-soil ecosystem. Researchers can conduct experiments across a range of scales, and compare experimental and numerical results to address the nation's most challenging problems related to contaminant transport, biogeochemical nutrient cycles, plant physiology, and plant-microbe-ecosystem interactions.

The resources include:

  • A plant growth laboratory with shared resources between PNNL and EMSL, where light, temperature, watering conditions, and CO2 concentration can be carefully controlled.
  • Microbial ecology laboratories for incubating cultures and communities under ambient or increased temperature/CO2 levels, and measuring their respiration and enzyme capacity.
  • Sample preparation for metatranscriptomics, metagenomics, high throughput amplicon sequencing, and other biogeochemical assays.
  • Custom flow cells at the pore- and intermediate- scales to study reactive and multiphase flow in saturated and undersaturated subsurface conditions.
  • Simulation of multiphase flow to design experiments, provide initial and boundary conditions, and to aid interpretation of results.
  • X-ray Computed Tomography for 3-dimensional imaging of the internal structure of environmental and other samples.
  • An analytical laboratory for C, H, N, S and inorganic assays.

Of course EMSL’s extensive range of instrumentation in other capabilities is available for further detailed analysis, including isolation of distinct cells and tissues, microscopy (e.g. fluorescence, electron, and atomic-force) as well as multiple -omic analyses.

See a complete list of instruments and laboratories available in the Plant, Soil & Subsurface Flow and Transport capability.

Description

Capability Details

  • Intermediate-scale columns and flow cells
  • Pore-scale microfluidic and imaging capabilities
  • High-pressure/temperature cells for research at reservoir conditions
  • Phytotron
  • Molecular Ecology Laboratory
  • Soil Incubation Laboratory
  • Dual energy gamma radiation system
  • Integrated hydraulic properties apparatus
  • Relative permeability apparatus
  • Analytical tools including ion, liquid and gas chromatographs, inductively coupled plasma-mass spectrometry and carbon analysis tools

Instruments

No instruments are available at this time.

Publications

Two-phase immiscible displacement in porous media is controlled by capillary and viscous forces when gravitational effects are negligible. The...
Dissolution of dense non-aqueous phase liquid (DNAPL) contaminants like tetrachloroethene (PCE) can be “bioenhanced” via...
As a remedial approach, vacuum-induced pore-water extraction offers the possibility of contaminant and water removal from the vadose zone, which may...
The objectives of this work were to determine if a porescale model could accurately capture the physical and chemical processes that control...
In this study, carbon dioxide exsolution from carbonated water is directly observed under reservoir conditions (9MPa and 45oC). Fluorescence...

Science Highlights

Posted: July 15, 2020
  A team of Pacific Northwest National Laboratory (PNNL) and EMSL scientists have compiled the most comprehensive genetic-level data set of the...
Posted: April 23, 2020
Microbial communities are vital to the health of soil ecosystems by performing critical functions like carbon and nutrient cycling. To better...
Posted: October 31, 2019
The Science Drought has plagued agriculture for centuries. Scientists suspect the reason plants survive under drought may have something to do with...
Posted: November 30, 2016
Researchers at the Department of Energy's Pacific Northwest National Laboratory injected carbon dioxide, or CO2, into basalt lava flows a half-mile...
Posted: June 03, 2016
Geochemists at Pacific Northwest National Laboratory used EMSL capabilities to help developed a mathematical formula to scale subsurface reaction...

Instruments

There are no related projects at this time.

The Plant, Soil, & Subsurface Flow and Transport Capability comprises a suite of resources for exploring physical and biogeochemical processes in the subsurface, and in the plant-soil ecosystem. Researchers can conduct experiments across a range of scales, and compare experimental and numerical results to address the nation's most challenging problems related to contaminant transport, biogeochemical nutrient cycles, plant physiology, and plant-microbe-ecosystem interactions.

The resources include:

  • A plant growth laboratory with shared resources between PNNL and EMSL, where light, temperature, watering conditions, and CO2 concentration can be carefully controlled.
  • Microbial ecology laboratories for incubating cultures and communities under ambient or increased temperature/CO2 levels, and measuring their respiration and enzyme capacity.
  • Sample preparation for metatranscriptomics, metagenomics, high throughput amplicon sequencing, and other biogeochemical assays.
  • Custom flow cells at the pore- and intermediate- scales to study reactive and multiphase flow in saturated and undersaturated subsurface conditions.
  • Simulation of multiphase flow to design experiments, provide initial and boundary conditions, and to aid interpretation of results.
  • X-ray Computed Tomography for 3-dimensional imaging of the internal structure of environmental and other samples.
  • An analytical laboratory for C, H, N, S and inorganic assays.

Of course EMSL’s extensive range of instrumentation in other capabilities is available for further detailed analysis, including isolation of distinct cells and tissues, microscopy (e.g. fluorescence, electron, and atomic-force) as well as multiple -omic analyses.

See a complete list of instruments and laboratories available in the Plant, Soil & Subsurface Flow and Transport capability.

Pages

Leads

Dr. Bowden manages EMSL's optical spectroscopy and diffraction, subsurface flow and transport, and microfabrication and deposition capabilities. He is responsible for the X-ray diffraction facility and assists or conducts measurement and analysis...