Environmental Molecular Sciences Laboratory

A DOE Office of Science User Facility

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Instruments

To help with proposal planning, icons in the table below indicate instrument availability:

  • 10 hours a day, 5 days a week
  • 24 hours a day, 7 days a week
Instrument Science Contact Description
300 MHz NMR Mazama (Solids)
Sarah Burton (509) 371-6535
David Hoyt (509) 371-6545
Highlighted Research Applications Molecular characterization of organic matter (OM) in soil systems, including comparisons between carbon species attributed to plant and microbial origin, and persistent carbon as a function of core depth and site environment. Organic-mineral fractions can be interrogated to determine the strength and mode of OM:mineral interaction (e.g., adsorbed versus chemically bound). Real-time monitoring of microbial metabolism under controlled conditions (similar to a micro-batch reactor)  such as temperature, pressure and head gas compositions to quantitatively and...
400 MHz NMR Karloff (Solids)
Nancy Washton (509) 371-7094
Andrew Lipton
Research Applications Characterization of quadrupolar nuclei for inorganic and biological materials and natural sediments Cryogenic NMR capabilities for 2H dynamics or wideline NMR of nuclei in dilute environments Characterization of terrestrial carbon in plant materials and soils. Researchers may operate the system independently on site or remotely. Most operations are standard, but users are strongly suggested to collaborate with EMSL scientists for cryogenic operations. Note: This spectrometer has limited access. Contact the instrument custodian for more details. Solid-state NMR probes:...
500 MHz NMR Shasta (Solids)
Andrew Lipton
Sarah Burton (509) 371-6535
Highlighted Research Applications Molecular characterization of organic matter (OM) in soil systems, including comparisons between carbon species attributed to plant and microbial origin, and persistent carbon as a function of core depth and site environment. Organic-mineral fractions can be interrogated to determine the strength and mode of OM:mineral interaction (e.g., adsorbed versus chemically bound). Real-time monitoring of microbial metabolism under controlled conditions (similar to a micro-batch reactor) such as temperature, pressure and head gas compositions to quantitatively and...
600 MHz NMR Baker (Liquids)
David Hoyt (509) 371-6545
Highlighted Research Applications EMSL’s suite of NMR metabolomics and metabolic flux analysis capabilities enables EMSL users to probe the metabolism of a diverse range of systems, from complex microbial communities present in soils to microbes bioengineered to produce next-generation biofuels and bioproducts. Our capabilities include: High resolution, high sensitivity NMR systems that provide one-dimensional and multidimensional data for biological samples.  Commonly observed nuclei include the biologically relevant proton (1H), carbon (13C), nitrogen (15N) and phosphorus (31P). NMR...
600 MHz NMR Hood (Metabolomics)
David Hoyt (509) 371-6545
Highlighted Research Applications EMSL’s suite of NMR metabolomics and metabolic flux analysis capabilities enables EMSL users to probe the metabolism of a diverse range of systems, from complex microbial communities present in soils to microbes bioengineered to produce next-generation biofuels and bioproducts. Our capabilities include: High resolution, high sensitivity NMR systems that provide one-dimensional and multidimensional data for biological samples.  Commonly observed nuclei include the biologically relevant proton (1H), carbon (13C), nitrogen (15N) and phosphorus (31P). NMR...
600 MHz NMR Nittany (Solids) - (Limited Time Available)
Nancy Washton (509) 371-7094
Kee Sung Han
Highlighted Research Applications This 600 MHz NMR system has the unique capacity to measure diffusion in both solid-phase and solution-phase samples utilizing high power gradients, in addition to traditional NMR analysis of solid-phase materials. The size of molecular aggregate in solution can be determined for dissolved organic matter (DOM) originating from soil or airborne particulates.  Guest-host binding interactions can be elucidated in protein-ligand and protein-protein associations. Determination of solid-phase plant cell wall structure and dynamics for analysis of treatment and...
600 MHz NMR Tumalo (Solids) - (Limited Time Available)
Nancy Washton (509) 371-7094
More details about this instrument will be available soon.
750 MHz NMR Bokan (Metabolomics)
Nancy Washton (509) 371-7094
Highlighted Research Applications High resolution, high sensitivity NMR systems that provide one-dimensional and multidimensional data for biological samples.Commonly observed nuclei include the biologically relevant proton (1H), carbon (13C), nitrogen (15N) and phosphorus (31P). NMR spectroscopy can provide unequivocal identification of isomers and can facilitate identification of unknowns. A unique EMSL-developed spectral database that enables simultaneous querying of multiple orthogonal data sources, including NMR and MS, to expand and confirm metabolite identifications in the complex...
750 MHz NMR Rainier (Liquids)
Nancy Washton (509) 371-7094
Highlighted Research Applications Analysis of dissolved organic matter from soils/sediments and waterways (both fresh and salt water).  The interpretation can provide both qualitative and quantitative assessments as a function of changing conditions. Measure and trace carbon and nitrogen through metabolic pathways by incorporating feedstocks enriched with stable NMR active isotopes (e.g., 13C or 15N) Characterization of enzymatic or chemical degradation of biopolymers such as lignin. Protein structure elucidation and protein-ligand interaction studies. Elements that are biologically and...
800 MHz Dynamic Nuclear Polarization (DNP) Ison - (avail. Summer 2020) More details about this instrument will be available soon.
800 MHz NMR Tava (Metabolomics) - (avail. Spring 2020) More details about this instrument will be available soon.
850 MHz NMR Ellis (Solids)
Andrew Lipton
Nancy Washton (509) 371-7094
Highlighted Research Applications Molecular characterization of ecosystem components including plant tissue and bulk soils Determination of solid-phase plant cell wall structure and dynamics for analysis of treatment and breakdown of cellulosic biomass. Real-time monitoring of microbial metabolism under controlled conditions such as temperature, pressure and head gas compositions to quantitatively and qualitatively measure concentration changes and identify metabolites. Comprehensive Multiphase (CMP) NMR for study of all phases of intact samples: liquid, gel-like, semi-solid and solid in a...
Aberration Corrected TEM, Atomic Resolution Microscope with EELS (JEOL ARM)
Scott Lea (509) 371-6233
Located in RadEMSL (Radiochemistry Annex), the JEM-ARM200CF transmission electron microscope (TEM) enables atomic resolution of biogeochemical spatial associations, mineral identification and oxidation state determination of radiological samples. The JEM-ARM200CF TEM enables structure analysis with atomic resolution in TEM and scanning transmission electron microscope (STEM) modes. Furthermore, it allows elemental and chemical bonding analysis of single atomic columns via energy-dispersive x-ray spectroscopy (EDS), and electron energy-loss spectroscopy (EELS) from Gatan and the Gatan digital...
Analytical: Chromatograph, Agilent
Mark Bowden (509) 371-7816
More details available soon.
Aquilos Cryo-FIB/SEM (avail. approx. Nov. 2020) More details about this instrument will be available soon.
Atom Probe Tomography
Daniel Perea (509) 371-6235
The LEAP® 4000 XHR local electrode atom probe tomography instrument enabled the first-ever comprehensive and accurate 3-D chemical imaging studies of low electrical conductivity materials, such as ceramics, semiconductors and oxides. The atom probe is assisting EMSL's efforts to further scientific advancements in interface analysis and microstructural characterization, providing a new tool for understanding the relationship between the nanoscale structure of materials and their macroscopic properties. It's also being used to advance aerosol research. EMSL's Atom Probe Offers: Unique...
Biological Atomic Force and Super Resolution Fluorescence Microscope
Galya Orr (509) 371-6127
Dehong Hu (509) 371-6126
The system combines the power of atomic force microscopy (AFM) and super resolution fluorescence microscopy to support studies of cell surface proteins and structures with 1-10 nm resolution while identifying specific proteins and molecular complexes by distinct fluorescent tags (i.e., fluorescent proteins or antibodies). The system incorporates fluorescence microscopy with single molecule detection sensitivity to support super resolution by STORM/PALM, which enables fluorescence imaging with 20-30 nm resolution. The 3-D topographic map of the cell surface, acquired by AFM, has the potential...
C, H, N, S Analyzer
Tom Wietsma (509) 371-6375
EMSL housess two instruments for measuring the quantities of Carbon, Nitrogen, Hydrogen, Sulfur, and Oxygen in solid or liquid samples: A CHNS/O Analyzer A TOC/TNb (Total Organic Carbon/ bound Total Nitrogen) Analyzer Users may use the single “C,H,N,S Analyzer” resource for analysis of these elements. The CHNS/O system is an Elementar VarioEL Cube. The analysis of CHNS can be performed on a single sample and the system is equipped with an autosampler for automated analysis of multiple samples. The measurement of oxygen in a sample requires a separate instrument configuration and therefore...
CARS/SRS Confocal Microscope More details on this instrument will be available soon.
Circular Dichroism Spectrometer
David Hoyt (509) 371-6545
More details about this instrument will be available soon.
Computing: Aurora (Data Archive) (GB)
Ryan Wright (509) 371-6105
Dave Cowley (509) 371-6471
Aurora, EMSL's scientific data archive, is a dedicated computer system specifically designed for long-term storage of data collected by EMSL instruments. It is available at no cost to EMSL users who are part of an active EMSL user project, and follows EMSL's Data Management policies. Aurora is safe Designed to safely & securely hold data on a long-term basis Stores multiple copies of data to protect from data loss in case of media failure Expertly maintained by skilled information systems professionals from EMSL's Molecular Sciences Computing Group Monitored continuously for optimum...
Computing: Cascade (Linux Cluster) - (avail. until Oct. 2020)
Lee Ann McCue 509/375-2912
Cascade's 23,000 Intel processors have 184,000 gigabytes of memory available, about four times as much memory per processor as other supercomputers. The additional memory will allow scientists to use the processors more efficiently for biology, computational chemistry, materials science and atmospheric aerosols research. Read more about our computing policies in our User Guide.
Confocal, FLIM & Multi-Photon Fluorescence Microscope
Galya Orr (509) 371-6127
Dehong Hu (509) 371-6126
This microscopy system integrates seamlessly nonlinear two-photon excitation, laser-scanning confocal microscopy, and fluorescence lifetime imaging (FLIM) in one fully automated upright configuration. Nonlinear two-photon excitation allows minimally invasive and deep-penetrating, high-resolution 3-D imaging of living systems. The FLIM supports quantitative fluorescence resonance energy transfer (FRET) for investigating molecular interaction dynamics in live cells. The system also includes differential interference contrast imaging (DIC) to provide cellular context to the fluorescence...
CryoTEM (Tecnai)
Alice Dohnalkova (509) 371-6515
This FEI Tecnai T-12 cryo-transmission electron microscope (TEM) complements EMSL's broader microscopy suite and JEOL 2010 analytical high-resolution TEM. The cutting-edge capabilities of the T-12 cryo-TEM include: (1) a high-tilt stage that allows image tilt series to be obtained for three-dimensional reconstruction using electron tomography, and (2) a cryostage for observing samples in a frozen-hydrated state. The T-12 cryo-TEM is primarily devoted to biological samples involving morphological and immuno-cyto chemistry studies, but it also supports imaging of samples such as soft materials...
CyTOF - Mass Cytometer
Galya Orr (509) 371-6127
The CyTOF is a mass cytometer that uses time-of-flight mass spectrometry to quantify the presence of metal-tagged antibodies in single cells in high throughput. The use of metal isotopes as tags allows high throughput screening of individual cells for the expression of more than 40 different proteins simultaneously. In EMSL, we established the tagging of fluorescence in situ hybridization probes with metal isotopes to quantify gene expression in individual bacterial cells in high throughput. This single cell analysis approach supports transcriptomic analysis of multiple genes in single...
Dynamic Force AFM (Asylum) The Asylum MFP-3D BIO is a versatile atomic force microscope (AFM) that combines molecular resolution imaging and picoNewton force-based measurements on an inverted optical microscope. It is designed specifically for biological applications and can be used to: Study individual protein or DNA molecules in situ Image the surface of living cells Measure the adhesive forces of proteins on cell surfaces Conduct single-molecule force spectroscopy of single DNA, protein, and polysaccharide molecules Conduct nanolithography and manipulation of samples on the nanometer and picoNewton scale. With the...
Dynamic TEM - (available approx. Oct. 2020)
James Evans (509) 371-6709
The Dynamic Transmission Electron Microscope, or DTEM, is under development at EMSL in collaboration with scientific colleagues at Pacific Northwest National Laboratory. Housed in EMSL's Quiet Wing, it will enable dynamic in situ gas or liquid observations of complex systems in real-time and under realistic conditions – at nanometer to atomic spatial resolution and microsecond to nanosecond temporal resolution. It will open the door to understanding: Protein conformational changes, Aging and transformations of aerosols, and Mechanisms of battery and catalytic function. This one-of-a-kind,...
Electron and Photon Stimulated Desorption (BES 2)
Greg Kimmel (509) 371-6134
EMSL's non-thermal interfacial reactions instrumentation is available for use in research directed toward understanding non-thermal interfacial processes. Much radiation damage occurs due to the many secondary electrons produced when high-energy radiation penetrates a material. Using this instrumentation, researchers can probe the details of radiation "damage" on both clean and adsorbate-covered surfaces by observing the effects of low-energy electron bombardment of samples. Studies of amorphous ice, as well as a newly developed liquid beam source, permit investigation of non-thermal...
Electron Microprobe
Bruce Arey (509) 371-6485
Housed in EMSL's RadEMSL (Radiochemistry Annex), the field emission electron microprobe (EMP) enables chemical analysis and imaging of radionuclides with high spatial resolution in radioactive samples. The EMP allows non-destructive imaging and compositional elemental analysis of solid-phase materials at high spatial resolution with parts per million sensitivity to aid in analysis of inorganic and radionuclide fate and transport in geochemical systems. Its cathodoluminescence capabilities affords identification of episodic growth in minerals. Initial experiments conducted on natural samples...
Electron Spectrometer: XPS with Laser Interface
Alan G Joly (509) 371-6158
This ultrahigh vacuum machine can be applied as a routine means for analyzing the structure and chemical state of surface and near-surface constituents with a spatial resolution of 10 μm and an energy resolution of less than 0.2 eV. It is equipped with state-of-the art charge neutralization and charge balance capability, so that not only conducting and semi-conducting materials may be sampled, but wide-band gap, non-conducting materials (minerals, metal-oxides, ceramics, etc.) also may be analyzed. Special modifications include several quartz and lithium fluoride laser ports, such that unique...
Environmental FIB/SEM (Quanta)
Odeta Qafoku (509) 371-6383
The environmental scanning electron microscope (ESEM) is a new-generation SEM that can image samples under controlled environments and temperatures. Three-dimensional section analysis morphology, focused ion beam, electron backscattering diffraction, and energy dispersive X-ray analysis make this a versatile instrument for the user program. This instrument provides added capability in SEM imaging with an advanced ability to analyze wet and insulating materials as well as samples at elevated temperatures under controlled gaseous environments. This instrument: Provides precise 3D imaging and...
Environmental TEM
Libor Kovarik (509) 375-4377
EMSL's environmental transmission electron microscope (ETEM) is a state-of-the-art, Cs-corrected field emission gun (FEG) scanning transmission electron microscope equipped with an Environmental Cell infrastructure for controlled gas inlet into the sample/holder area and analysis of the chemical composition of the atmosphere in this area. The ETEM will allow researchers to perform microscopic and spectroscopic in situ measurements relevant to catalytic and geochemical studies important to energy and environmental applications and is expected to answer important questions about the interfacial...
Eukaryotic Cell Culture
Galya Orr (509) 371-6127
The cell culture facility is adjacent to the microscopy suite, where bio-hoods, CO2 incubators, benchtop centrifuges, 37°C water baths and a shakers are available for cell growth and manipulations. The facility supports the growth of eukaryotic primary cells or cell-lines, including human cell lines.
Fluorescence Spectrometer, Time-Correlated Single Photon Counting
Alan G Joly (509) 371-6158
More details about this instrument will be available soon.
Fluorimeter
Zheming Wang (509) 371-6349
EMSL's Horiba Fluorolog 3 is a high-sensitivity fluorescence spectrometer used for measuring excitation and emission spectra of solids and solutions. The instrument is equipped with a Hamamatsu R29 photomultiplier tube for measurements in the ultraviolet and visible and a Hamamatsu R5509-73 liquid nitrogen-cooled photomultiplier tube covering the broad spectral range from 250 nm -1700 nm. The instrument's built-in light source consists of a 450-W Xe lamp with a quartz envelope for strong ultraviolet and visible wavelengths. The spectrometer is equipped with a dual grating monochromator (f/4.1...
FTIR Microscope
Zheming Wang (509) 371-6349
A Bruker Fourier Transform Infrared (FTIR) spectrometer (IFS66/S) with visible, near- and mid-infrared (IR) capabilities is available at EMSL. It is equipped with a microscope with an approximate resolution of 60 μm, sample cooling and heating attachments, an MCT detector and a digital camera for image capture. The microscope stage has manual and motor drive capabilities for sample mapping. A host of sampling accessories are available and described below. With these unique resources, infrared spectra of various different species can be obtained. Examples of work using FTIR at EMSL includes:...
GC-MS, Volatile Organic Compounds
Tom Wietsma (509) 371-6375
More details about this instrument willl be available soon.
Geochemistry AFM (Icon)
Scott Lea (509) 371-6233
The Geochemistry Atomic Force Microscopy (AFM) is able to measure the structural, topographic, electrical, and magnetic properties of a wide range of mineral surfaces and affixed microorganisms. The new AFM has multiple sample environments. It can image in ambient conditions and fluids and will be equipped with a sample heating and cooling stage with a range of at least -30°C to 200°C. The software interface, scan speeds, and image resolution represent significant upgrades from existing instrument. Additionally, it has the following imaging modes: contact, intermittent contact, phase imaging...
Helios FIB/SEM
Odeta Qafoku (509) 371-6383
The FEI Helios Nanolab dual-beam focused ion beam/scanning electron microscopy (FIB/SEM) microscope combines two important high-resolution microscopy tools that jointly enable important nanoscale measurements and the unique preparation of samples and materials for other uses or analysis. This instrument will provide rapid and selected region preparation of samples for transmission electron microscopy (TEM) and other spectroscopies; three dimensional analyses of nanoscale materials and other small objects; and the capability for nanoscale lithography. Functionally, the instrument includes...
Helium Ion Microscope
Shuttha Shutthanandan (509) 371-6530
Scott Lea (509) 371-6233
The Helium Ion Microscope promises to advance biological, geochemical, biogeochemical, and surface/interface studies using its combined surface sensitivity and high-resolution microscopy functions. The HIM is equipped with a Rutherford Backscattering Spectrometry (RBS) capability that identifies atomic elements. In the future, the RBS capability will allow 3-D chemical analysis when paired with an improved spectrum detector. EMSL'S Helium Ion Microscope Offers: Ultra-high resolution — reveals fine structure details and allows chemical visualization of nanostructures and biological samples,...
High Resolution Microprobe XPS
Mark Engelhard (509) 371-6494
The Physical Electronics Instruments (PHI) Quantum 2000 X-ray photoelectron spectrometer (XPS) is a unique system that uses a focused monochromatic aluminum K X-ray beam that can be varied in size from as small as 10 μm in diameter to approximately 200 μm. The beam can be rastered over larger areas (about 0.5 mm2). The PHI Quantum 2000 XPS features: A focused X-ray beam (10 μm to 200 μm) Beam raster capability to millimeter sizes Optical- (charge-coupled diode camera) and X-ray-generated electron images (scanned X-ray image-SXI) to select areas of analysis A special specimen holder for angle-...
High Resolution, Ultrafast SFG Vibrational Spectroscopy
Mark Bowden (509) 371-7816
Zheming Wang (509) 371-6349
Recently built and only available at EMSL, this customized SFG-VS spectrometer employs the synchronization of a 100-picosecond amplifier laser, which provides unprecedented spectral resolution, and a <40 femtosecond amplifier laser, which provides sufficient ultrabroad infrared spectral coverage, making it the first instrument in the world to provide <1 cm-1 spectral resolution in the surface vibrational spectrum with sub-monolayer sensitivity. In comparison to scanning systems, the broadband SFG-VS spectrometer provides fast and accurate line shape measurement in the surface...
Ice Nucleation Chamber PNNL’s custom-built ice nucleation chamber is available to EMSL users to study the ice nucleating properties of atmospheric particles or aerosols in-situ under user-defined conditions. These conditions are designed to simulate mixed-phase and cirrus clouds, and generate data to reduce the uncertainty related to these clouds in predicting the Earth’s radiation budget and climate. The nucleation section of the chamber consists of two parallel plates that are controlled and maintained at a desired supercooled temperature to generate defined ice supersaturated conditions. Aerosols are drawn into...
ICP-MS (Quadrupole)
Tom Wietsma (509) 371-6375
Ryan Kelly (509) 371-6525
The Agilent 7500 Series inductively coupled plasma mass spectrometer (ICP-MS) is available for all research areas requiring analysis of trace metals in liquid samples. The ICP-MS is equipped with Agilent’s Octopole Reaction System (ORS) to eliminate interferences from the plasma and sample matrix. The system has an autosampler but can also be interfaced with a liquid chromatograph for speciation studies. Sample Preparation Options The laboratory that houses this instrument contains the necessary support items for sample preparation, including auto pipettes, a balance, nanopure water, and...
Imaging XPS
Mark Engelhard (509) 371-6494
Shuttha Shutthanandan (509) 371-6530
The X-ray photoelectron spectroscopy (XPS) imaging system provides fast, uantitative, real-time parallel imaging with highest resolution spectroscopy at all analysis areas. In addition, the system is equipped with temperature programmed desorption (TPD), ultraviolet photoelectron spectroscopy (UPS), ion scattering spectroscopy (ISS), a cluster/Ar+ ion gun, and a five-axis automated stage with variable temperature sample environment at the analysis chamber. The system is also integrated with a radial distribution chamber (RDC), a full-size glove box, and a cold-stage at the sample introduction...
Influx - Flow Cytometer Cell Sorter
Galya Orr (509) 371-6127
The Influx, a flow cytometer/cell sorter, provides 5 laser lines simultaneously, powerful detection capability and diverse sorting approaches for high throughput analysis and sorting of distinct cells or organelles from complex samples or cell populations. For example, users can sort cells by their proportional hybridized mRNA content or lipid production, or by the expression of specific proteins or pigments. The system incorporates an advanced multi-parameter sorting technology and supports multiple collection options, including sorting directly into micro-titer plates, slides, or Petri...
Intermediate Flow Cells
Tom Wietsma (509) 371-6375
Researchers in the Intermediate Flow Cell laboratory. A custom cell with multiple sampling ports can be seen at left, positioned for dual-gamma imaging. EMSL builds custom flow cells at the pore-  and intermediate- scales to study multiphase flow in saturated and undersaturated subsurface conditions. The intermediate flow cells are built to meet individual experimental needs and are packed with media of differing porosity/permeability, arranged to simulate geological features relevant to specific research questions. Groundwater or other flows are introduced under controlled conditions, and...
Inverted Confocal Raman Spectrometer
Mark Bowden (509) 371-7816
More details about this instrument will be available soon.
Ion Chromatograph
Tom Wietsma (509) 371-6375
This ion chromatography capability comprises two Dionex DX-500 modular chromatography systems that can be configured to conduct fully automated analysis of anions, organic acids, inorganic cations, ammonium, amines, and metals. The systems feature quaternary gradient pumps; autosamplers; thermostated column compartments; and conductivity, electrochemical, and ultraviolet detectors. The systems are also equipped with online eluent generators that eliminate the preparation of common eluents used in ion analysis. A wide variety of columns are available that can be optimized for specific...
Laser Ablation Sampling System
Mary Lipton (509) 371-6589
More details about this instrument will be available soon.
Laser Capture Microdissection (LCM)
Dehong Hu (509) 371-6126
This Laser Capture Microdissection system is equipped with 100 x objective lens for enriching distinct organelles, or isolating single cells from cell populations or tissue sections, including cryosections or paraffin sections, as well as fresh tissue, such as plants, for further omics or imaging analyses. The system is integrated within a high-end wide-field fluorescence microscope that provides multiple excitation and emission channels and high sensitivity CCD camera to enable the detection of distinct organelles or cells within complex samples. Figure caption: High resolution laser capture...
Laser Desorption Analysis
Alan G Joly (509) 371-6158
EMSL offers a suite of instrumentation dedicated to understanding photoreactivity in the condensed phase, on surfaces, and at material interfaces. In addition, a variety of continuous wave and pulsed laser sources are available that are capable of providing tailored photoexcitation from the extreme ultraviolet through mid-infrared. Pulse durations of these instruments range from several nanoseconds to less than 100 femtoseconds. Laser desorption and ablation from surfaces is a powerful experimental tool used for studying the energetics and dynamics of the interaction of molecules with...
Laser Desorption Analysis
Alan G Joly (509) 371-6158
EMSL offers a suite of instrumentation dedicated to understanding photoreactivity in the condensed phase, on surfaces, and at material interfaces. In addition, a variety of continuous wave and pulsed laser sources are available that are capable of providing tailored photoexcitation from the extreme ultraviolet through mid-infrared. Pulse durations of these instruments range from several nanoseconds to less than 100 femtoseconds. Laser desorption and ablation from surfaces is a powerful experimental tool used for studying the energetics and dynamics of the interaction of molecules with...
Lattice LightSheet Microscope - (avail. Jan. 2021) More details about this instrument will be available soon.
Live Cell Single Molecule Fluorescence Microscope
Galya Orr (509) 371-6127
This time-lapse fluorescence microscope with single molecule detection sensitivity is used to follow individual molecules or organelles in their native intact cellular environment over time. This system enables the study of dynamic processes at the molecular level to understand mechanisms underlying cellular functions. Constructed by EMSL scientists using commercial components, the system consists of multiple lasers coupled to a high-end wide-field microscope, a total internal reflection (TIRF) module, and a CCD camera with single molecule detection sensitivity. The system also supports...
Low Temperature Photoelectron Spectroscopy
Alan G Joly (509) 371-6158
This experimental facility couples an electrospray ionization source to a magnetic-bottle time-of-flight photoelectron spectrometer. It has been developed to investigate multiply charged anions and solution phase species in the gas phase. A low-temperature electrospray ion source, in which a solution sample containing the anions is sprayed through a syringe needle under negative bias, produces multiply charged anions. This facility features a low temperature ion trap, which can control ion temperature between 10 and 400K. This has significantly expanded our research capability and flexibility...
Low Temperature UHV STM/AFM
Zdenek Dohnalek (509) 371-6150
EMSL's ultra-high vacuum, low-temperature scanning probe microscope instrument, or UHV LT SPM, is the preeminent system dedicated to surface chemistry and physics at low temperatures down to 5 K. Operating at low temperatures provides high mechanical stability, superior vacuum conditions, and negligible drift for long-term experiments. With thermal diffusion being entirely suppressed, stable imaging becomes possible even for weakly bound species. The system is primarily used for probingsingle-site chemical reactivity, while the combination with a hyperthermal molecular beam allows the study...
Mass Spectrometer: 21T FTICR
Ljiljana Paša-Tolić (509) 371-6585
Mary Lipton (509) 371-6589
EMSL’s 21 Tesla Fourier transform ion cyclotron resonance mass spectrometer, or 21T FTICR MS, is one of only two in the world with this high of a magnetic field strength. The 21T FTICR MS significantly enhances EMSL’s overall mass spectrometry analytical and characterization performance in terms of sensitivity, dynamic range, accuracy, resolution and speed. This unprecedented level of performance is needed to unravel the chemistry and dynamics of complex natural systems in the environment. This transformative instrument enables the characterization of large, intact proteins and complex...
Mass Spectrometer: Aerosol, time-of-flight, high resolution
Mary Lipton (509) 371-6589
The Quadrupole Aerosol Mass Spectrometer (QAMS) manufactured by Aerodyne Inc., was added as a capability in the EMSL user facility in 2004 and the Time-of-Flight Aerosol Mass Spectrometer (TOF-AMS) in 2005. The AMS quantitatively measures the size and chemical composition of volatile/semi-volatile submicron aerosols. The AMS is designed to provide quantitative composition information on ensembles of particles. The QAMS combines a linear quadrupole mass spectrometer and standard vacuum techniques with recently developed aerosol sampling techniques to provide limited single particle information...
Mass Spectrometer: Fourier-Transform Ion Cyclotron Resonance
Ljiljana Paša-Tolić (509) 371-6585
Mary Lipton (509) 371-6589
More details on this  instrument will be available soon.
Mass Spectrometer: FT-ICR, 6T (Ion Surface Collisions)
Mary Lipton (509) 371-6589
The 6-Tesla High-Field Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FT-ICR MS), is a unique instrument designed and constructed at EMSL. It was designed to incorporate surface-induced dissociation (SID) as an excitation method for complex ions. The instrument is equipped with a high-transmission electrospray ionization source (ESI) and an intermediate pressure matrix assisted laser desorption ionization (MALDI) source for efficient ionization of large molecules. The ESI source consists of an ion funnel, a mass-resolving quadrupole, and an external ion accumulation cell. Ions...
Mass Spectrometer: GC QExactive
Mary Lipton (509) 371-6589
Details about instrument will be available soon.
Mass Spectrometer: GC-MS (metabolomics)
Ljiljana Paša-Tolić (509) 371-6585
Mary Lipton (509) 371-6589
More details about this instrument will be available soon.
Mass Spectrometer: Imaging (MALDI) More details about this instrument will be available soon.
Mass Spectrometer: Inductively Coupled Plasma (ICP-MS) System
Mary Lipton (509) 371-6589
This high-resolution ICP-MS complements the isotopic capabilities of the Neptune by providing highly accurate and sensitive elemental concentrations of relevant samples. The high resolution of the Element II allows reduction of molecular interferences that can limit ICP-MS accuracies without collision cell or other similar methods. The high efficiency interface and the double-focusing ion optics of the Element II system additionally and importantly enable the ultimate in ICP-MS detection sensitivities to be attained (part-per-quadrillion level concentration detection limits are achievable)....
Mass Spectrometer: Ion Mobility Spectrometry, Time of Flight
Mary Lipton (509) 371-6589
We have several separation platforms combining liquid chromatography (LC) and ion mobility spectrometry (IMS) based separations with TOF mass spectrometry for increased throughput and sensitivity in proteomics, metabolomics, lipidomics, natural organic matter, and others. These platforms are typically targeted for science questions requiring higher throughput analysis of biological systems or a structural based separation or analysis. Each separation technology, LC, IMS, and TOF-MS, are tailored to the specific application including optimized resolution, sensitivity, and throughput....
Mass Spectrometer: LC Triple Quadrupole
Mary Lipton (509) 371-6589
More details about this instrument will be available soon.
Mass Spectrometer: Linear Ion Trap Quadrupole (LTQ) Orbitrap MS - for environmental research (nanoDESI)
Mary Lipton (509) 371-6589
Nano-DESI/HRMS analytical platform allows in-depth molecular characterization of very small samples of organic materials  (down to 10 ng) and volumes while maintaining high sensitivity and acquisition of mass spectra at high mass resolution. HPLC-PDA/HRMS platform is used for focused studies of light absorbing environmental organics. Both platforms are used in a variety of environmental research projects relevant to atmospheric aerosol chemistry, aquatic and soil chemistry, MS imaging of biological samples. Research Applications Atmospheric Aerosols Chemistry – molecular level...
Mass Spectrometer: Orbitrap More details about this instrument will be available soon.
Mass Spectrometer: Proton Transfer Reaction (PTRMS)
Mary Lipton (509) 371-6589
EMSL's field-deployable Proton Transfer Reaction Mass Spectrometer (PTR-MS) is capable of real-time, online quantification of volatile organic compounds in air. The instrument provides an extremely fast response time of about one second, and detection limits for a one-second integration time range from 50 pptv to about 300 pptv, depending on the nature of the analyte. Organic species are measured using chemical ionization mass spectrometry. H3O+ is used as the reagent ion and results in a soft ionization of most organic species with negligible fragmentation. The air sample is continuously...
Mass Spectrometer: Single Particle (SPLAT II)
Alla Zelenyuk (509) 371-6155
Mary Lipton (509) 371-6589
SPLAT II is a second-generation Single Particle Laser Ablation Time-of-Flight mass spectrometer. A high-precision and unique instrument, it allows users to study the fundamental processes that govern the chemistry and physics of particles on the nano- and micro-scales. SPLAT II's applications include but are not limited to climate, air pollution, human health, bioterrorism, and emerging nanotechnologies. Portability is a hallmark of SPLAT II—it is the first field-deployable instrument that provides, in real time, the size, density, shape, fractal dimension, and composition of individual...
Mass Spectrometer: Time of Flight Secondary Ion (ToF SIMS) - 1997
Mary Lipton (509) 371-6589
The Physical Electronics Instruments (PHI) Model T2100 time-of-flight secondary ion mass spectrometer (TOF-SIMS) is used to perform research activities such as: Identifying organic overlayers or contamination spots or layers on diverse surfaces ranging from mineral grains to silicon chips Simultaneous mapping of the surface distribution of elements or molecules within an area of 10 x 10 to 400 x 400 microns or more, often in combination with identifying elements or molecules Identifying depth distribution of elements within materials such as thin films or in particles deposited on a substrate...
Mass Spectrometer: Time of Flight Secondary Ion (ToF SIMS) - 1997
Mary Lipton (509) 371-6589
  The Physical Electronics Instruments (PHI) Model T2100 time-of-flight secondary ion mass spectrometer (TOF-SIMS) is used to perform research activities such as: Identifying organic overlayers or contamination spots or layers on diverse surfaces ranging from mineral grains to silicon chips Simultaneous mapping of the surface distribution of elements or molecules within an area of 10 x 10 to 400 x 400 microns or more, often in combination with identifying elements or molecules Identifying depth distribution of elements within materials such as thin films or in particles deposited on a...
Mass Spectrometer: Waters Synapt G-2 More details about this instrument will be available soon.
Mass Spectrometer: Waters Synapt G-2 More details about this instrument will be available soon.
Mass-Selected Ion Deposition System - Electrospray Source
Mary Lipton (509) 371-6589
The mass-selected ion deposition system is a new instrument constructed at EMSL. The apparatus, shown in Figure 1, includes a high-transmission electrospray ion source, quadrupole mass filter, bending quadrupole that deflects the ion beam and prevents neutral molecules originating in the ion source from impacting the surface, an ultrahigh vacuum (UHV) chamber for ion deposition by soft landing, and a vacuum-lock system for introducing surfaces into the UHV chamber without breaking vacuum. Its design allows us to control soft landing energies (0-500 eV) and spot sizes from around 5 mm down to...
Microbeam XRD
Tamas Varga (509) 371-6042
Mark Bowden (509) 371-7816
EMSL's microbeam x-ray diffractometer comprises a Rigaku rotating anode source with micro focus optics, an automated x-y stage, and a curved image plate detector. A Cr anode is used to avoid a high fluorescence background from iron-containing specimens. The specialized optics and collimators allow spatial resolution between 10 and 500 microns. In addition to the common XRD applications of compound identification, unit cell measurement, and crystallite size determination, the microbeam diffractometer can also provide maps of the orientations of crystallographic directions within the beam area...
Microfluidics and Microfabrication
Ryan Kelly (509) 371-6525
EMSL’s microfluidics and microfabrication suite includes the ability to design, fabricate, evaluate and model microfluidic devices and other miniaturized constructs. Microfluidic devices are made from a variety of materials including glass, silicon, polydimethylsiloxane (PDMS) and other polymers. PDMS is an elastomeric material that enables active flow control to be incorporated into the microfluidic devices using integrated microvalves. These custom devices can be used for a wide variety of applications such as: Creating engineered, perturbable microenvironments for culturing unicellular...
Molecular Beam Epitaxy
Scott Chambers (509) 371-6517
Tim Droubay (509) 371-6488
EMSL's molecular beam epitaxy (MBE) deposition system is used for the synthesis and characterization of novel oxide, ceramic, and mineral materials as crystalline films. These materials are of significant interest in a variety of scientific and technological fields, including electronics, magnetics, magneto-optics, photonics, thermal and photo- catalysis, and geochemistry. EMSL's MBE deposition system consists of a PNNL-designed, customized MBE chamber with: An electron cyclotron resonance plasma gas source for O2 and/or N2 An ozone (O3) collection and delivery unit Four electron-beam...
Molecular Beam Epitaxy, Multi-source
Yingge Du (509) 371-6512
Scott Chambers (509) 371-6517
Tim Droubay (509) 371-6488
Resembling Sputnik, the ozone- or oxygen-plasma-assisted molecular beam epitaxy system is designed to enable the growth of crystalline metal, alloy, or oxide films of transition metal and select alkaline earth elements with an unprecedented degree of stoichiometric control.  The versatile deposition chamber is connected to a surface analytical chamber via an ultra-high vacuum (UHV) transfer line.  Integral to the entire system is a combination loadlock and sample preparation chamber for sample introduction and preparatory conditioning. Multiple samples can be housed under UHV conditions...
Molecular Beam Kinetics
Scott Smith (509) 371-6156
Molecular beam scattering from surfaces is a powerful experimental tool for studying the dynamics and kinetics of the interaction of molecules with surfaces. The coupling of surface science, molecular beam, and laser technologies makes possible the measurement of total energy disposal and redistribution in gas-surface scattering. Previously, these experimental methods were used to acquire detailed surface kinetics and state-to-state scattering measurements of molecules interacting with metallic substrates. While such experiments have resulted in a fairly detailed understanding of surface...
Molecular Ecology Laboratory
Kirsten Hofmockel 509-371-6444
More details available soon.
Mossbauer Spectrometer
Ravi Kukkadapu (509) 371-6384
More details about this instrument will be available soon.
Multimode AFM (Nanoscope)
Scott Lea (509) 371-6233
The Digital Instrument (DI) Nanoscope IIIa multimode scannning probe microscope is primarily used to obtain topographical information on nanostructures, nanodevices, and quantum dots; thin films (oxide surfaces, spintronics, magnetic storage, and semiconductors); geological and geochemical samples; protein and biomolecule structures at surfaces; and samples undergoing electrochemical and corrosion processes. Resolution ranges from the submicron-length scale (nanoscale) to the atomic level. This powerful instrument allows the user to obtain surface roughness statistics, image small nanosized...
Multipurpose XRD
Tamas Varga (509) 371-6042
Mark Bowden (509) 371-7816
All work involving the following systems and any experiments conducted in EMSL laboratories must be performed in compliance with EMSL practices and permits. The special applications X-ray diffractometer is a PANalytical X'Pert MPD system with a vertical Θ-Θ goniometer (220 mm radius). The X-ray source is a long-fine-focus, ceramic X-ray tube with Cu anode. Normal operating power is 45 kV, 40 mA (1.8 kW). This instrument is equipped with two beam paths: focusing and parallel beam optics. The focusing optics consist of programmable divergence, anti-scatter, and receiving slits; incident and...
Nano-FTIR
Scott Lea (509) 371-6233
The scattering-type near-field optical microscope is truly a one-of-a-kind, state-of-the-art high resolution chemical imaging system developed through a partnership with Professor Markus Raschke at the University of Colorado-Boulder. It is designed to provide spectroscopic infrared vibrational near-field nanoimaging that provides ultrahigh spatial resolution down to 10 nm, vibrational spectral information in the 700 to 5000 cm-1 (14 to 2 µm) range, sensitivity down to the molecular level, and applicability under ambient and environmental conditions. This instrumentation enables temperature...
NanoSIMS
Zihua Zhu (509) 371-6240
John Cliff (509) 371-7624
EMSL's new-generation ion microprobe (NanoSIMS) extends high spatial resolution secondary ion mass spectrometry analyses to extremely small areas (down to 50 nm) and volumes while maintaining high sensitivity at high mass resolution (e.g., ppm in elemental imaging while resolving isobaric interferences). NanoSIMS can measure up to seven secondary ions in parallel, offering high-quality isotopic ratio results from the same small volume while maintaining perfect image superimposition. NanoSIMS can be used for enhanced, simultaneous, nanoscale imaging of elements/isotopes of inorganic and...
Photoemission Electron Microscope
Alan G Joly (509) 371-6158
EMSL's photo-emission electron microscope (PEEM) is capable of imaging nanoscale surface structures ( ~ 8 nm) via electron emission induced by ultraviolet and laser light sources. The PEEM is applied to surface science studies of individual nanostructures and catalytic sites on specially designed metal, semi-conducting, and metal oxide substrates. Because these sites are of nanometer dimension, EMSL selected the highest resolution PEEM available from the manufacturer, Elmitec. The Elmitec PEEM uses inherently low-aberration magnetic lens elements. A complementary suite of ultrafast lasers...
Physical Property Measurement System
Mark Bowden (509) 371-7816
Tim Droubay (509) 371-6488
The Physical Property Measurement System (PPMS) from Quantum Design Inc. is a versatile, open architecture, variable temperature-field system, designed to perform a variety of automated advanced thermal-transport, electro-transport, and magneto-transport measurements on diverse samples in fields up to 9 Tesla. The PPMS may be used with options designed for it or easily adapted to individualized unique user-defined experiments. Sample environment controls include fields up to ± 9 Tesla and temperature range of 1.9 - 400 K. EMSL’s PPMS instrument has a number of additional modules which allow...
Plant Sciences Laboratory
Christer Jansson 509/371-6516
EMSL's Plant Sciences Laboratory, an enclosed research greenhouse used for studying interactions between plants and the environment, provides walk-in and reach-in chambers for growth of small and medium-stature plants under highly controlled conditions of light, temperature, humidity, and CO2 levels (enrichment and scrubbing). Soil-based, as well as hydroponic plant-growth, systems are available. Watch this video to learn how the Plant Sciences Laboratory can accelerate your research. Brachypodium distachyon tested under different environmental conditions in EMSL's Plant Sciences Laboratory.
Pore Scale Micromodels
Tom Wietsma (509) 371-6375
The pore-scale micromodel flow and transport lab is part of EMSL's Subsurface Flow and Transport Laboratory (SFTL) with a focus on coupled (multiphase) flow, diffusion, and reactions processes at the microscopic scale (μm to cm) that affect the fate and transport of contaminants in the subsurface and geological sequestration of CO2. The pore-scale micromodel laboratory features a variety of tools for scientific users, including: A HORIBA Jobin Yvon Raman spectroscopy system with 532- and 632-nm lasers combined with a Nikon Eclipse Ti epifluorescence microscope for mineral and microbe...
Pulsed Laser Deposition
Tim Droubay (509) 371-6488
EMSL's pulsed laser deposition (PLD) system is designed for epitaxial growth of oxide, ceramic, or synthetic mineral thin films and is composed of the following: An electro-polished stainless-steel chamber pumped via a turbo molecular pump and backed by a rotary scroll dry pump An excimer laser (KrF) for excitation at 248 nm Three standard mass flow control units for precise and reproducible process gas flow during deposition An electron cyclotron resonance (ECR) plasma gas source Reflection high-energy electron diffraction (RHEED) for real-time monitoring of the surface structure and...
Pulsed/CW X-Band (9.5 GHz) EPR
Eric Walter (509) 371-6873
The Bruker Elexsys 580 electron paramagnetic resonance (EPR) spectrometer performs continuous wave and pulsed magnetic resonance using electron spins to selectively focus on certain types of molecules, such as free radicals and paramagnetic metal complexes, while avoiding background from the other components of the sample. Research using this spectrometer focuses on in situ experiments, making it a capability that is unique for user facilities.  As such, the instrument provides the researcher with the ability to: Obtain structural and redox information for samples containing paramagnetic...
Radiological AFM EMSL's Digital Instruments Radiological BioScope™ Atomic Force Microscope (AFM) allows radiological samples to be examined in fluids or air with little or no sample preparation and can be used to examine heterophase processes on mineral surfaces and to image three dimensional topography of radiological samples. The microscope supports all major AFM-imaging techniques and modes, including contact and intermittent contact (TappingMode™) force-volume, lateral force, force modulation, phase, lift, etc. The controller enables simultaneous acquisition through up to four independent data acquisition...
Radiological FIB/SEM (Quanta)
Scott Lea (509) 371-6233
Odeta Qafoku (509) 371-6383
The environmental scanning electron microscope (ESEM) is a new-generation SEM that can image samples under controlled environments and temperatures. Three-dimensional section analysis morphology, focused ion beam, electron backscattering diffraction, and energy dispersive X-ray analysis make this a versatile instrument for the user program. This instrument provides added capability in SEM imaging with an advanced ability to analyze wet and insulating materials as well as samples at elevated temperatures under controlled gaseous environments. This instrument: Provides precise 3D imaging and...
Radiological Powder XRD
Mark Bowden (509) 371-7816
The Rigaku Miniflex 600 diffractometer housed in RadEMSL (EMSL’s radiochemical annex) is a benchtop instrument suitable for identification and structural analysis of a range of crystalline materials. It operates in a coupled θ-2θ mode to provide standard powder diffraction patterns from a variety of sample forms. EMSL’s expertise and diffraction software are available to provide detailed analysis, including: Phase identification, from the most recent ICDD database of more than 350,000 patterns Measurement of lattice parameters Size/strain analysis based on line broadening Quantitation and...
Radiological XPS
Mark Engelhard (509) 371-6494
Housed in RadEMSL (EMSL's Radiochemistry Annex), the AXIS Ultra X-ray Photoelectron Spectrometer (XPS) integrates a magnetic immersion lens and charge neutralization system with the spherical mirror and concentric hemispherical analyzers. The hemispherical analyzer includes a delay-line detector (DLD) for state-of-the-art spectroscopic energy resolution and detection sensitivity. Small spot capabilities (<15µm) are achieved via a series of selected area apertures used in combination with the magnetic and electrostatic lenses. In parallel imaging mode photoelectrons are transferred to the...
Scanning Probe AFM Compound Microscope
Dehong Hu (509) 371-6126
The atomic force microscope (AFM) compound microscope is designed primarily for fluorescence imaging in the study of nanoscale chemical processes, such as interfacial electron transfer and organic thin film devices. This high-resolution, highly sensitive instrument provides correlated optical and topographical images, with the image resolution beyond the optical diffraction limit. The instrument is equipped with a Nikon inverted microscope (Model: Diaphot); Digital Instrument AFM (Model: Bioscope); raster scanning device (constructed by EMSL scientists); and single photon counting detectors,...
Scanning TEM
Chongmin Wang (509) 371-6268
EMSL's aberration-corrected Titan 80-300™ scanning/transmission electron microscope (S/TEM) provides high-resolution imaging with sub-angstrom resolution and spectroscopic capabilities. This state-of-the-art instrument is equipped with a Schottky field-emission electron source, an electron gun monochromator, a CEOS hexapole spherical aberration corrector for the probe-forming lens, a high-angle annular dark-field (HAADF) detector, an X-Ray Spectrometer (EDS) and a high-resolution GIF. The selection of electron energy between 80kV and 300kV enables optimized imaging for a variety of materials...
Sequencing, Next Generation, High Throughput - Ion S5
Lye Meng Markillie
The Ion systems are designed for high-throughput sequencing using semiconductor technology that directly translates chemical information into digital data, eliminating the need for optics and complex sequencing chemistries. EMSL currently has two Ion Proton™ and one Ion S5 systems together with two Ion Chef™ systems for sample preparation. In EMSL, we use the Ion systems mainly for RNA-Seq, with a focus on meta-transcriptomic analysis of complex microbial communities, and whole transcriptome analyses of plants, fungi and other organisms relevant to bioenergy, climate change or carbon cycling...
Sequencing, Next Generation, High Throughput - Ion Torrent Proton (transcriptomics)
Galya Orr (509) 371-6127
The Ion Proton™ systems are designed for high-throughput sequencing using semiconductor technology that directly translates chemical information into digital data, eliminating the need for optics and complex sequencing chemistries. EMSL currently has two Ion Proton™ and one Ion S5 systems together with two Ion Chef™ systems for sample preparation. In EMSL, we use the Proton systems mainly for RNA-Seq, with a focus on meta-transcriptomic analysis of complex microbial communities, and whole transcriptome analyses of plants, fungi and other organisms relevant to bioenergy, climate change or...
Sequencing, Next Generation, High Throughput - NextSeq550
Lye Meng Markillie
Illumina's Next Generation Sequencing system (NextSeq550C) is currently incorporated in users' research for transcriptomic analysis (RNA-Seq). The system is used to study gene expression profiles, metabolic pathways and molecular processes underlying functions of microbial cells, communities, fungi and plants and the interactions between them. Global transcriptome analysis can be done in individual eukaryotic cells (single cell RNA-Seq) or in complex microbial communities (meta-transcriptomics), as well as in single organism cultures or plants. The system, which provides unbiased,...
Sequencing, Next Generation, High Throughput Ion S5 Prime More details about this instrument will be available soon.
SIM - Super Resolution Fluorescence Microscope & Confocal Airyscan
Galya Orr (509) 371-6127
PNNL's super resolution fluorescence structured illumination microscope, or SIM, allows users to gain new insights into living cells with 120-130 nanometer lateral resolution by overcoming the diffraction limit of light. Classified as a super resolution fluorescence microscope, SIM acquires images with a spatial resolution that is nearly two folds higher than the 250-300 nanometer resolving power of the classical confocal fluorescence microscope. SIM allows researchers to image live or intact hydrated cells in 3-D in their near native form. A recent addition to the super resolution...
Single-Molecule Fluorescence Microscope
Dehong Hu (509) 371-6126
The single-molecule o fluorescence microscope is designed to study complex reaction dynamics such as enzymatic reactions, protein-protein interactions, and interfacial electron transfer process. It is designed and adapted specifically for single-molecule spectroscopy experiments. Constructed by EMSL scientists, this instrument comprises an inverted optical microscope, a variety of continuous-wave and pulsed lasers, optics, spectrometers, and ultrasensitive photon detectors. Researchers use this instrument to study a variety of samples in solid phase and solution. For solid-phase experiments,...
Soil Hydraulic Property Measurement
Tom Wietsma (509) 371-6375
More details about this instrument will be available soon.
Soil Hydraulic Property Measurement
Tom Wietsma (509) 371-6375
More details will be available soon.
Stopped-Flow Absorbance/Fluorescence Spectrometer
Zheming Wang (509) 371-6349
The Bio-Logic® SFM-400/S is a 4-syringe stopped-flow system that offers the capability to carry out complex, multi-mixing experiments with the lowest rates of aging and deadtimes. The 4-syringe system offers a minimum deadtime of 0.25 ms with a microcuvette. In tests conducted in EMSL using regular cuvettes, an instrument deadtime of less than 1 ms with the FC08 (0.8-mm light pathlength) or ~ 2 ms with the FC10/100 (10-mm light pathlength) was easily achieved. The small sample volume of less than 0.03 ml makes the system ideal for experiments such as those involving biological reactants for...
STORM/PALM - Super Resolution Fluorescence Microscope
Galya Orr (509) 371-6127
Dehong Hu (509) 371-6126
EMSL has developed and offers fluorescence, super resolution STORM (stochastic optical reconstruction microscope), also known as PALM (photo-activated localization microscopy). STORM is a fully automated super-resolution fluorescence microscope that enables fluorescence imaging of the intact cell with nanometer resolution. It incorporates single-molecule fluorescence techniques to reconstruct super-resolution images using software that automatically acquires and analyzes data in real time. Figure caption: Intracellular organelles are detected inside intact hydrated cells with ~30 nm...
Sum Frequency Generation for Surface Vibrational Spectroscopy
Zheming Wang (509) 371-6349
Mark Bowden (509) 371-7816
This customized SFG-VS spectrometer incorporates unique polarization and incident angle control to enable versatile measurement of the surface vibrational spectroscopy of various molecular interfaces with submonolayer sensitivity. The spectral resolution of this system is about 4-6 cm-1, significantly better than most other broadband SFG-VS spectrometers, which typically have a spectral resolution of ˜15 cm-1. This robust and easy-to-use system is meant to work together with the FemtoPico HR-BB-SFG-VS spectrometer for routine SFG-VS measurements and screening samples. Work published in the ...
Time-of-Flight SIMS
Zihua Zhu (509) 371-6240
Secondary ion mass spectrometry (SIMS) is a high-vacuum surface analysis technique. With ppm to ppb level detection limits, it is amongst the most sensitive surface analysis tools. The sample surface is bombarded with energetic (normally, 10-30 keV) primary ions and the secondary ions generated by this process separated and detected with a time-of-flight (ToF) analyzer. The mass spectra provide critical information about elemental, isotopic and molecular components of the sample surface. The EMSL ToF-SIMS instrument is equipped with an Argon cluster ion (Arn+) gun, a liquid metal ion gun with...
Transient Kinetic Analysis
Janos Szanyi (509) 371-6524
EMSL recently constructed a transient kinetic analysis (TKA) apparatus (in collaboration with the University of Washington) to elucidate reaction mechanisms and identify the rate-determining steps in heterogeneous catalysis for processes related to reactions of the hydrogen economy. Systems of interest include, but are not limited to, reforming of small oxygenates, PROX, and water-gas shift chemistries. These investigations are made possible using an apparatus that can perform a transient interrogation of catalyst-adsorbed species by rapid-scan transmission Fourier-transform ion resonance (...
Variable Temperature UHV STM/AFM
Zdenek Dohnalek (509) 371-6150
The Omicron variable temperature ultra-high vacuum (UHV) scanning tunneling microscope (VTSTM) is designed to study the structure of both clean and adsorbate-covered solid surfaces. As a real space probe, emphasis is placed on examining defect structures and understanding their role in heterogeneous chemical processes. Sample temperatures during imaging can nominally range from 30 K to 1500 K. At low temperatures, even weakly bound adsorbates can be imaged due to the reduced thermal diffusion. Sample Preparation, Handling, and Characterization The system is equipped with a sample load-lock...
X-ray Computed Tomography
Tamas Varga (509) 371-6042
Mark Bowden (509) 371-7816
X-ray computed tomography (XCT) produces 3-dimensional computer models of the entire volume of samples. Contrast between different portions is made on the basis of how strongly they absorb x-rays, which is typically a function of elemental composition and density. The technique is generally non-destructive and reveals the internal architecture of specimens, including pores, inclusions, and other internal features. The large sample chamber and the various X-ray targets available allow considerable flexibility including in situ experiments of fluid displacement in pore networks and examination...
Zeta Potential Analyzer
Mark Bowden (509) 371-7816
More details will be available soon.