Environmental Molecular Sciences Laboratory

A DOE Office of Science User Facility

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User Projects

The following is a list of projects that have been active over the past three years.
For a comprehensive list of projects, see All Projects. You can also search for released data.
Lead Institution: Mayo Clinic
Principal Investigator: Rajiv Kumar
Cellular DNA is damaged in the course of normal cellular metabolic activities and by environmental factors such as chemicals and ultraviolet light that can cause DNA lesions. This DNA lesion damage is repaired by special translesion synthesis (TLS...
Lead Institution: Duke University
Principal Investigator: Seok-Yong Lee
The SLC superfamily of membrane transporters play critical roles in many aspects of human physiology and pharmacology. Several SLC transporters play roles in drug transport. Of note, nucleoside transporters are important contributors to nucleoside...
Lead Institution: University of Kansas Medical Center
Principal Investigator: Bret Freudenthal
Despite the packaging of eukaryotic DNA into chromatin through repeating units known as the nucleosomes, it is constantly damaged via reactive oxygen species (ROS). 8oxo-guanine (8oxoG) is a common form of DNA damage resulting from the oxidation of...
Lead Institution: University of British Columbia
Principal Investigator: Natalie Strynadka
Type III Secretion Systems (T3SS) are multiprotein, syringe-shaped nanomachines that deliver virulence factors, known as effector proteins, from bacteria into host cells. T3SS are common in Gram-negative bacteria and are key for the progression of...
Lead Institution: University of Wisconsin, Madison
Principal Investigator: Paul Ahlquist
SARS-CoV-2, Zika, Dengue, and many other dangerous viruses belong to the positive-strand RNA((+)RNA) viruses. Our group first used cryo-electron tomography (cryo-ET) to reveal the organization of viral RNA and protein components in membrane-bounded...
Lead Institution: University of Utah
Principal Investigator: Peter Shen
The cytosolic chaperonin CCT is a large, 1 MDa protein folding machine that plays a central role in maintaining the cellular proteome. CCT contributes to proteome homeostasis by assisting in the folding of a large set of proteins with complex folds...
Lead Institution: University of British Columbia
Principal Investigator: Natalie Strynadka
The ongoing COVID-19 epidemic represents a global public health crisis unseen in recent memory. The virion surface glycoprotein known as spike is a trimeric extension that mediates both the initial interaction and the subsequent fusion with host...
Lead Institution: Sao Carlos Institute of Chemistry, University of Sao Paulo
Principal Investigator: Julio Borges
Chaperones, such as Hsp100, can help maintain cellular homeostasis and adaptation playing an important role for different organisms. The Hsp100 has a disaggregase action, acting with other proteins of the chaperone system to extract polypeptides...
Lead Institution: University of Utah
Principal Investigator: Christopher Hill
Our goal is to determine the structure of heptameric AAA+ ATPase Bcs1 bound to its native substrate Rip1. Rip1 is a critical component of the Complex III of mitochondrial electron transport chain. To be incorporated into Complex III, Rip1 first...
Lead Institution: University of British Columbia
Principal Investigator: Natalie Strynadka
Wall teichoic acid (WTA) is a Gram-positive bacterial cell wall polymer with roles in host cell adherence and coordination of essential physiological processes including cell division and peptidoglycan synthesis. Importantly, the biogenesis of WTA...