Environmental Molecular Sciences Laboratory

A DOE Office of Science User Facility

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User Projects

The following is a list of projects that have been active over the past three years.
For a comprehensive list of projects, see All Projects. You can also search for released data.
Lead Institution: Fred Hutchinson Cancer Research Center
Principal Investigator: Barry Stoddard
Bacterial defense against phage infection is comprised of a large number of biological mechanisms and systems. A thorough understanding of such systems is crucial both for anti-bacterial phage therapy and for the use of microbial expression...
Lead Institution: Northwestern University
Principal Investigator: Yuan He
Eukaryotic genomes are modulated by different types of chromatin remodeling complexes and transcribed by multiple RNA polymerases. Of the three polymerases, Pol I-III, that produce RNA required for protein production, key structural information for...
Lead Institution: Boston Children's Hospital
Principal Investigator: Hao Wu
The inflammatory response provides an absolutely essential host defense mechanism that, if perturbed, can itself cause a large number of human diseases. Collectively, innate immune responses integrate a number of genome-encoded sensor proteins to...
Lead Institution: University of Texas Southwestern Medical Center
Principal Investigator: Yuh Chook
Core histones organize DNA in the nucleus. Histones are synthesized in the cytoplasm and are be transported by to the nucleus by Karyopherin nuclear transport receptors. In the cytoplasm, histones also bind to histone chaperones, which often...
Lead Institution: Northwestern University
Principal Investigator: Amy Rosenzweig
Particulate methane monooxygenase (pMMO) is the primary enzyme used for methane metabolism by methane-consuming bacteria known as methanotrophs. pMMO is a copper-dependent, membrane-bound metalloenzyme that oxidizes methane to methanol by an unknown...
Lead Institution: Yale University
Principal Investigator: Yong Xiong
Human immunodeficiency virus type 1 (HIV-1) infection is a global epidemic. Between the point of entry into the cell and integration of the HIV-1 genome into the host genome, the virus encounters many host cofactors, primarily interacting with the...
Lead Institution: Northwestern University
Principal Investigator: Yuan He
Among all different types of DNA damages, double strand breaks (DSBs) are viewed as the most toxic ones that lead to genome instability. Non-homologous end-joining (NHEJ) pathway, in which the two broken DNA ends are directly ligated without...
Lead Institution: Fred Hutchinson Cancer Research Center
Principal Investigator: Betty Shen
Nuclease enzymes are of enormous importance for a wide range of biological and biotechnology disciplines that require site-specific DNA manipulations. Bacterial R-M systems consist of a restriction endonuclease that specifically cleaves an...
Lead Institution: Oregon Health & Science University
Principal Investigator: Show-Ling Shyng
The ATP-sensitive potassium (KATP) channel formed by four Kir6.2 and four SUR1 subunits regulate insulin secretion in ?-cells by coupling glucose metabolism with membrane excitability. Mutations in the ?-cell KATP channel causes abnormal insulin...
Lead Institution: Emory University
Principal Investigator: Eric Ortlund
Nuclear receptors (NRs) sense changes in lipid metabolite levels to trigger specific patterns of gene regulation, which drive critical biological processes including metabolism, development, and inflammation. Liver receptor homolog-1 (LRH-1) is a NR...