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2020 EMSL User Meeting
October 5 - 8, 2020

Overview

The 2020 EMSL User Meeting—Visualizing the Proteome, was held virtually from October 5-8.

This 3-day meeting encompassed the following topics: 

  • Pushing the frontier of structural biology 

  • Understanding subcellular compartmentalization and ultrastructure 

  • Annotating protein function and modifications via native and top-down proteomics 

  • Revealing cellular complexity and dynamics with single to few-cell proteomics 

  • Advancing cellular models and protein design with high-performance computing 

Attendees included international researchers from academia, industry, and government research laboratories. The EMSL User Meeting is open to EMSL users and non-users alike. 

2020 EMSL User Meeting: Day One

The first day of the 2020 EMSL User Meeting included:

  • Session 1: Pushing the frontier of structural biology with integrated approaches, chaired by EMSL Chemist James Evans.
  • EMSL Chemist James Evans presents "Vision for EMSL visual proteomics."
  • EMSL Chemist, Dusan Velickovic, presents "Molecular Snapshots of complex environmental biochemistry," a discussion of the research that led to his 2020 MT Thomas Award for Outstanding Postdoctoral Achievement.
  • Tutorial 1: Cryogenic Electron Microscope, presented by EMSL Chemist Trevor Moser.

Meeting welcome and introduction

Gap junction channels visualized in a dynamic lipid environment

Structural basis for strand transfer inhibitor binding to HIV intasomes

Pterocarpan synthase (PTS) structures suggest a common quinone methide-stabilizing function in dirigent proteins with dirigent-like domains

Vision for EMSL Visual Proteomics

Molecular snapshots of complex environmental biochemistry

Single particle cryo-electron microscopy tutorial

2020 EMSL User Meeting: Day Two

The second day of the 2020 EMSL User Meeting included:

  • Session 2: Peering inside cells to understand subcellular compartmentalization and ultrastructure, chaired by EMSL Chemist Trevor Moser
  • Session 3: Revealing cellular complexity and dynamics with single to few cell proteomics, chaired by EMSL Chemist Ying Zhu.
  • Tutorial 2: Few-cell proteomics, presented by EMSL Chemist Ying Zhu.

Recent developments in whole cell cryo-electron tomography

Leveraging Super High Optical Resolution Microscopy to Probe the Interaction Zone Between Clostritium thermocellum and Biomass

Endophyte-Promoted Phosphorous Solubilization in Populus

Microfluidic sample preparation for EM Protein purification, single cell analysis, and visual proteomics

Few-cell proteomics

2020 EMSL User Meeting: Day Three

The third day of the 2020 EMSL User Meeting included:

  • Session 4: Annotating protein function and modifications via native and top-down proteomics, chaired by EMSL Chemist Mowei Zhou
  • Virtual poster session and networking
  • Tutorial 3: Native mass spectrometry, presented by EMSL Chemist Mowei Zhou

How unique is your plasma proteome? A personalized perspective

Towards Protein Structures from Native Mass Spectrometry & Single Particle Imaging

Automated capillary isoelectric focusing-tandem mass spectrometry for qualitative and quantitative top-down proteomics

Interactome studies in aging and heart failure

Native Mass Spectrometry tutorial

2020 EMSL User Meeting: Day Four

The fourth day of the 2020 EMSL User Meeting included:

  • Session 5: Advancing cellular models and protein designs with scientific computing, chaired by EMSL Computational Scientist Aivett Bibao
  • Tutorial 4: Scientific computing, presented by EMSL Computational Scientist Aivett Bibao

Studying Quantitative Protein Compaction, Unfolding, and Dissociation with Ion Mobility-Mass Spectrometry

The application of machine learning for the identification of protein signatures from APT data

Scientific Computing tutorial