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Molecular Observation Network

Soils contain more carbon than the atmosphere and all plants, combined. This comprises the second largest pool of carbon at Earth’s surface.

The role of soils in the global carbon cycle is a crucial one. However, the ecological processes that govern whether carbon remains in soils or is released into the atmosphere are complex. These unknowns make it complicated for scientists to predict and mitigate climate change.

To improve the understanding of the molecular and microscale processes around carbon in soils, the scientific community needs to standardize molecular and microscale soil data across regions.

It’s with these scientific questions in mind that the Environmental Molecular Sciences Laboratory (EMSL) has developed the Molecular Observation Network, or MONet.

What will MONet do?

MONet aims to transform understanding of soils and provide news tools for adapting to and counteracting climate change.

This database will allow for the conversion of traditionally labor-intensive molecular analysis methods into a high-throughput workflow, providing key molecular and microscale information to climate scientists, modelers, and experimenters.​ By standardizing these workflows, all data will be useful and can reduce human bias and uncertainties.​ 

Vast amounts of unique data will be collected and managed at an unprecedented scale and used to advance regional-scale and Earth system process models that cover key ecosystems across the United States. MONet data will be made available to the community in the database linked to analytical tools that enable access by modelers, experimenters, and field scientists. A key aspect of MONet is incorporating biogeochemical and microbial process data streams to facilitate parameterization of key variables of multiscale models with eventual coupling to larger-scale Earth system models. Learn more in the data section.

 

MONet Data Accessibility Development Plan February 2023 to October 2024

To create data for the database, EMSL is collecting thousands of soil samples from across the continental United States.​ 

Please consider submitting to a quarterly soil submission call as available. We invite national laboratory researchers, academic researchers, citizen scientists, and undergraduate and graduate students from universities to participate. 

There is no cost for participation other than submitting samples. EMSL is covering the cost of the analyses, and you will receive more than 20 different advanced data types to use in your research.

2024 MONet Sampling Call Schedule 

Spring 2024 Sampling: 

  • Proposal Submission: January 2, 2024, to February 1, 2024 
  • Decision Notice: Mid-March 2024 
  • Sampling Period: April 2024 to May 2024 

Summer 2024 Sampling:

  • Proposal Submission: February 14, 2023, to April 1, 2024 
  • Decision Notice: mid-May 2024 
  • Sampling Period: June 2024 to August 2024 

Fall 2024 Sampling: 

  • Proposal Submission: June 3, 2024, to July 2, 2024 
  • Decision Notice: Mid-August 2024 
  • Sampling Period: September 2024 to November 2024 

Winter 2024 Sampling: 

  • Proposal Submission: Sept 3, 2024, to Oct 1, 2024 
  • Decision Notice: mid-November 2024 
  • Sampling Period: December 2024 to February 2025 

Who can be involved with MONet?

EMSL is collaborating with a broad range of partners managing an expanding network of selected natural, urban, and managed watershed, coastal, continental, and atmospheric sites, both experimental and observational. We invite national laboratory researchers, academic researchers, citizen scientists, and undergraduate and graduate students from universities to participate.

Training Resources

2023 Molecular Observation Network Community Science Meeting

EMSL LEARN Webinar Series: MONet Proposal Submission: Tips for Success

  • Learn details for MONet's quarterly soil sample submissions calls.

EMSL LEARN Tutorials: Molecular Observation Network Series

  • Explore how to submit a proposal to an open call and how to collect a soil sample.

The MONet Team

John Bargar

      John Bargar, EMSL
      Overall questions

  Emily Graham

      Emily Graham, EMSL
                 Omics

  Odeta Qafoku

        Odeta Qafoku, EMSL
            Soil interfaces

Yuri Corilo

       Yuri Corilo, EMSL 
         Data analysis

  Sarah Leichty

         Sara Leichty, EMSL
   EMSL project management

  Tanja Woyke

           Tanja Woyke, JGI
     Deputy for User Programs

Christa Pennacchio

    Christa Pennacchio, JGI
    JGI project management

       

Strategic Partners

 

Joint Genome Institute logo

The Joint Genome Institute (JGI) will perform and prepare sequencing, metagenome analyses, assembly files, annotation, and bins of MONet samples. Data will be available for download from the EMSL MONet database and through JGI’s portals. 

National Science Foundation's National Ecological Observatory Network logo

The National Science Foundation's National Ecological Observatory Network will provide a national network of field and observational sites to facilitate sampling and continuous sensing opportunities. 

National Microbiome Data Collaborative

The National Microbiome Data Collaborative will support accessibility of MONet microbiome metadata.

Bowman et. al. 2023. "One thousand soils for molecular understanding of belowground carbon cycling." Frontiers in Soil Science, Sec. Soil Biogeochemistry & Nutrient Cycling, 3. https://doi.org/10.3389/fsoil.2023.1120425

Bowman et al. 2023. "Molecular Observation Network (MONet)." protocols.io. https://dx.doi.org/10.17504/protocols.io.5jyl8jr49g2w/v2

Review the MONet Data Access Instructions.

The data and models generated through MONet will improve prediction of ecosystem function and response to disturbances, supporting the long-term U.S. Department of Energy (DOE) goal of scientifically informed decision-making regarding the nation’s energy and environmental security and sustainability. A beta data visualization tool has been created to help people interact with the data collected in the 1000 Soils Project. We are actively working to improve visualizations and value all feedback. 

Data Being Collected by the Molecular Observation Network

Data Types and Training 

Submitted soil samples will be analyzed using an extensive suite of advanced techniques to produce different data types, including the following: 

  • High-resolution molecular composition of soil organic matter 

  • Metagenomic sequencing 

  • 3-D microstructure 

  • Dissolved solutes 

  • Respiration, microbial biomass, and potential enzyme activity. 

 

Resources for users to understand their data include the webinars available on the EMSL website and JGI website

Data Availability

Data will be provided to the submitter through the EMSL User Portal (NEXUS). Consistent with open science principles, data will be published to EMSL’s database, where it will be publicly available without embargo according to the EMSL Data Policy. Review instructions for accessing MONet data information. For metagenome data generated at the JGI, filtered reads, assemblies, annotations, and metagenome-assembled genomes (MAGs) will be provided for download on the JGI Data Portal. Metagenome data will also be made available through the Integrated Microbial Genomes and Metagenomes platform for comparative analysis. 

Participate in the MONet Contributor’s Consortium 

The MONet Data Consortium is a citable authorship group that contains a collective of authors who have contributed soil cores for analysis and have opted into participation. Consortium participants will be listed as collaborators on papers where the MONet Data Consortium is cited as an author. 


How to Acknowledge EMSL and JGI for MONet Data 

“Soil data were provided by the Molecular Observation Network (MONet) at the Environmental Molecular Sciences Laboratory (https://ror.org/04rc0xn13), a DOE Office of Science user facility sponsored by the Biological and Environmental Research program under Contract No. DE-AC05-76RL01830. The work (proposal: 10.46936/10.25585/60008970) conducted by the U.S. Department of Energy, Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science user facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231. 

The Molecular Observation Network (MONet) database is an open, FAIR, and publicly available compilation of the molecular and microstructural properties of soil. Data in the MONet open science database can be found at https://www.emsl.pnnl.gov/monet.”