Alexander Johs

A model system to obtain structural information from the bacterial-mineral interface by neutron reflectivity

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Alexander Johs

Project type

Limited Scope

Electron transfer to insoluble electron acceptors by dissimilatory metal-reducing bacteria is facilitated by a pool of c-type cytochromes associated with the outer bacterial membrane. Recent studies…

A model system to obtain structural information from the bacterial-mineral interface by neutron reflectometry

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Alexander Johs

Project type

Large-Scale EMSL Research

Electron transfer to insoluble electron acceptors by dissimilatory metal-reducing bacteria is facilitated by a pool of c-type cytochromes associated with the bacterial outer membrane. Recent studies…

Nuclear magnetic resonance spectroscopy structure determination of the cobalamin binding protein HgcA required for bacterial mercury methylation

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Alexander Johs

Project type

Exploratory Research

Mercury is a pervasive global pollutant. Its methylated form, methylmercury, poses a substantial threat to human health. Methylation of mercury (Hg) by anaerobic bacteria is the primary source of…

NMR structure determination of the cobalamin binding protein HgcA and the ferredoxin-like protein HgcB required for bacterial mercury methylation

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Alexander Johs

Project type

Large-Scale EMSL Research

Mercury is a pervasive global pollutant. Its methylated form, methylmercury, poses a substantial threat to human health. Methylation of mercury (Hg) by anaerobic bacteria is the primary source of…

Identification of protein interaction networks, cofactors and protein structures essential for mercury methylation in anaerobic bacteria

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Alexander Johs

Project type

Large-Scale EMSL Research

Mercury is a pervasive global pollutant. Methylation of mercury (Hg) by anaerobic bacteria is the primary source of toxic methylmercury, which bioaccumulates up trophic levels and affects humans…

Study of Electron Transfer between Bacteria and Mineral

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Jim Browning

Project type

Limited Scope

The aim of this study is to determine the structural arrangement of proteins and conformational changes on a mineral surface under physiologically relevant conditions. This is important for a…

Single molecule FISH for the quantitative detection of mRNA transcripts targeting genes relevant for mercury methylation in Desulfovibrio desulfuricans ND132

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Alexander Johs

Project type

Limited Scope

Mercury (Hg) methylation by anaerobic bacteria and archaea is the primary source of toxic methylmercury (MeHg), which bioaccumulates up trophic levels and affects humans through consumption of fish…

Quantification of gene expression patterns relevant to microbial mercury transformations

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Alexander Johs

Project type

Large-Scale EMSL Research

Levels of toxic methylmercury (MeHg) in the environment are a net result of mercury (Hg) methylation and MeHg demethylation processes. Mercury methylation by anaerobic bacteria and archaea is the…

EMSL Science Overview

Alexander Johs

Projects

A model system to obtain structural information from the bacterial-mineral interface by neutron reflectivity

A model system to obtain structural information from the bacterial-mineral interface by neutron reflectometry

Nuclear magnetic resonance spectroscopy structure determination of the cobalamin binding protein HgcA required for bacterial mercury methylation

NMR structure determination of the cobalamin binding protein HgcA and the ferredoxin-like protein HgcB required for bacterial mercury methylation

Identification of protein interaction networks, cofactors and protein structures essential for mercury methylation in anaerobic bacteria

Study of Electron Transfer between Bacteria and Mineral

Single molecule FISH for the quantitative detection of mRNA transcripts targeting genes relevant for mercury methylation in Desulfovibrio desulfuricans ND132

Quantification of gene expression patterns relevant to microbial mercury transformations