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Alice Dohnalkova

Institution
Environmental Molecular Sciences Laboratory

Projects

Host Cell Interactions with Human Infectious Diseases, Straub

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Timothy Straub
Project type
Large-Scale EMSL Research
Pathogenesis is a complex biological process between the human host and the infectious disease agent. In our previous successful collaboration with EMSL scientists (EMSL Project 16297), which lead…

Extracellular Research Thrust for EMSL SSA

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Kirsten Hofmockel
Here we propose to address the basic geochemical and biogeochemical interactions that mediate organic-mineral interactions. Identifying binding mechanisms at the organic matter-mineral interface will…

Special Projects in Electron Microscopy

Lead Institution
David Heil Associates Corporation
Principal Investigator
James Young
Project type
Exploratory Research
WSU-TriCities course offering for practical training in one or more areas of electron microscopy: TEM, SEM, ultramicrotomy, specimen processing, darkroom procedures and light microscopy. Involves…

Extracellular Research Thrust for EMSL SSA

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Kirsten Hofmockel
This project will develop a conceptual framework that defines the biotic and abiotic mechanisms regulating the persistence of mineral associated organic matter (OM). The specific goals are to…

Electron microscopy imaging of cyanobacteria Synechcystis sp.

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Alice Dohnalkova
Project type
Grand Challenge
As a part of Grand Challenges in Membrane biology, Synechocystis sp. will be investigated in regards of cell morphology and elemental analysis of minerals associated with their outer and inner…

Microscopic visualization of membrane pores and bacterial foulants

Lead Institution
University of Houston
Principal Investigator
Shankar Chellam
Project type
Exploratory Research
My research is mainly centered on membrane technologies for municipal water purification. We (1) elucidate contaminant transport and membrane fouling mechanisms, (2) devise methods to enhance…

Biogenic Iron Materials

Lead Institution
J. Craig Venter Institute
Principal Investigator
Yuri Gorby
Project type
Exploratory Research
We will image iron minerals produced by the reduction of solid phase ferric oxides. The research will require the use of field emission SEM and TEM.

Uranium Biomineralization by Caulobacter crescentus

Lead Institution
Lawrence Livermore National Laboratory
Principal Investigator
Yongqin Jiao
Project type
Large-Scale EMSL Research
The research goal of this proposal is to understand and evaluate the potential of using the aerobic bacterium Caulobacter crescentus for the application of uranium bioremediation. Previous work by…

The in vivo localization and interactions between structural components of the Shewanella oneidensis MR-1 metal reducing system by high resolution transmission electron microscopy.

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Matthew Marshall
Project type
Grand Challenge
To understand the mechanisms of anaerobic metal oxide reduction, important issues regarding the subcellular localization and the outer membrane architecture of the iron-reducing respiratory chain…

The role of microbes in biomineral polymorph selection

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Alice Dohnalkova
Biologically precipitated minerals are commonly present in very close association with biomineralizing microbes. Microbe-mineral interactions are complex in that they are often reciprocal in nature…

Decreasing the amount of material required for gross biological carbon and nitrogen flux measurements by more than 12 orders of magnitude using ion implantation isotope dilution and stable isotope measurements by NanoSIMS

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
John Cliff
Advances in mass spectrometry coupled to stable isotope probing techniques have profoundly influenced our understanding of process level biology and biogeochemistry. When stable isotope compounds and…

Proteomic studies of growth and metabolism in Corynebacterium glutamicum R

Lead Institution
Research Institute of Innovative Technology for the Earth
Principal Investigator
Hideaki Yukawa
Project type
Exploratory Research
Corynebacterium glutamicum is a soil bacterium isolated in Japan in the 1950's as an L-glutamic acid producer (Kinoshita, et al., 1957). Strains derived from C. glutamicum are used in a number…

Functional Materials for Separation and Sensing

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Jay Grate
Project type
Large-Scale EMSL Research
A large number of mission areas of the US. DOE demand functional materials that will meet the operational requirements of targeted applications. Indeed, innovative solutions to pressing national…

TEM and SEM investigation of non-pathogenic bacterial cultures.

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Alice Dohnalkova
Project type
Exploratory Research
Trasmission and scanning electron microscopy is a key element in investigations of the morphology and reduction products that occur during exposure of bacteria to various experimental environments…

INTERACTIONS OF RARE EARTH ELEMENTS AND MICROORGANISMS

Lead Institution
Idaho National Laboratory
Principal Investigator
Yoshiko Fujita
Project type
Limited Scope
The overall goal of our research is to elucidate the mechanisms by which rare earth elements (REE) exert effects on environmental microorganisms. Global growth in new electronics and renewable…