Alice Dohnalkova

Regulatory dynamics of microbial communities

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

H Wiley

Project type

Large-Scale EMSL Research

This is a proposal to conduct work in EMSL in support of the Foundational Scientific Focus Area (FSFA) research program at Pacific Northwest National Laboratory. This large-scale research program…

Quantifying the functional and structural states of dynamic microbial communities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Fredrickson

Project type

Large-Scale EMSL Research

The overall goal of this proposal is to provide a quantitative framework in which to compare species-resolved patterns of gene expression, allowing comparison of the effects of perturbations of…

Elucidating synergistic plant-bacteria metabolic pathways for alleviating iron deficiency

Lead Institution

Oregon State University

Principal Investigator

Rene Boiteau

Nutrient mobilization from soil minerals is critical for plant growth, particularly in marginal lands with high pH soils or low phosphate availability. One way that rhizospheric bacteria enhance…

Establish a Penn State Center for Environmental Kinetics - as part of the Environmental Molecular Science Institute (EMSI) program (NSF/DOE-OBER) [PNNL Scope #47287, Zachara EMSI)

Lead Institution

Pennsylvania State University

Principal Investigator

William Burgos

Project type

Exploratory Research

The National Science Foundation has awarded a team of Penn State researchers $6.7 million to establish a new center for the study of environmental kinetics. Susan L. Brantley, director of the…

3d reconstruction of bacterial extracellular material for investigation of membrane turnover during metal reduction.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Dissimilatory metal reducing bacteria can immobilize soluble metals such as Fe, U or Tc by reducing them into less soluble material. This presents an elegant method for immobilization of motile toxic…

Rhizosphere Underground: Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Microorganisms are fundamental to biogeochemical processes, as microbial metabolism significantly contributes to the regulation of terrestrial carbon and nitrogen cycling. However, the impact of the…

A new capability for high-resolution 3D imaging of soil structure and soil chemistry in the rhizosphere

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Tamas Varga

We aim to answer the following critical questions about the rhizosphere: (i) What is the distribution of important nutrient elements like P in the soil and around the root? (ii) How much of the…

Characterization of novel arsenic-iron precipitates formed during biological iron reduction

Lead Institution

Colorado State University

Principal Investigator

Thomas Borch

Project type

Exploratory Research

Arsenic (As) is a priority pollutant, the fate of which is in part controlled by reactions with ferrous iron. Ferrous iron is capable of mediating solid phase transformations of soil and sediment…

Geobiology of Chemical Weathering: Spatially Explicit Characterization of the Inorganic Chemical Composition of Mycorrhizospheric Biofilms

Lead Institution

Washington State University

Principal Investigator

Chester Keller

Project type

Large-Scale EMSL Research

Rich literatures and recent advances across several fields have set the stage for process-based research into the biogeochemical agency of vascular plants -- in particular, how their physiologies…

Multi-Omics Evaluation of Tripartite Associations in Red Alder Roots: Integrated Transcriptomics, Proteomics, Metabolomics and Metabolite Imaging of Red Alder Symbiotic Associations in Root Tissue

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

This proposal presents the unique opportunity to conduct the first ever integrated multi-omics and imaging evaluation of the Alnus rubra (red alder tree)/Frankia alni (bacteria)/Alpova diplophloeus …

Structure and Chemical Mapping of Soil Organic Matter on Mineral Surfaces

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ravi Kukkadapu

Secreted and decomposing soil organic matter (SOM) containing carbon, nitrogen, phosphorus, and metals form the basis of many bio/organic matter-mineral interactions in soils. These compounds are …

EMSL Science Overview

Alice Dohnalkova

Projects

Regulatory dynamics of microbial communities

Quantifying the functional and structural states of dynamic microbial communities

Elucidating synergistic plant-bacteria metabolic pathways for alleviating iron deficiency

Establish a Penn State Center for Environmental Kinetics - as part of the Environmental Molecular Science Institute (EMSI) program (NSF/DOE-OBER) [PNNL Scope #47287, Zachara EMSI)

3d reconstruction of bacterial extracellular material for investigation of membrane turnover during metal reduction.

Rhizosphere Underground: Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

A new capability for high-resolution 3D imaging of soil structure and soil chemistry in the rhizosphere

Characterization of novel arsenic-iron precipitates formed during biological iron reduction

Geobiology of Chemical Weathering: Spatially Explicit Characterization of the Inorganic Chemical Composition of Mycorrhizospheric Biofilms

Multi-Omics Evaluation of Tripartite Associations in Red Alder Roots: Integrated Transcriptomics, Proteomics, Metabolomics and Metabolite Imaging of Red Alder Symbiotic Associations in Root Tissue

Structure and Chemical Mapping of Soil Organic Matter on Mineral Surfaces