Alice Dohnalkova

Novel flow based biomolecule immobilization on nanoporous scaffolds
for enhanced biosensor performance and drug delivery applications

Lead Institution

University of Washington

Principal Investigator

Amy Shen

Project type

Exploratory Research

Biosensors play indispensible roles in disease diagnosis, drug screening, and forensic applications. One important area of biosensor research is the immobilization of biomolecules (i.e. enzyme, DNA)…

Rhizosphere Underground:
Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Microorganisms are fundamental to biogeochemical cycling, as microbial metabolism significantly contributes to the regulation of terrestrial carbon and nitrogen cycling. However, the impact of the…

Influences of Hydrologic Exchange Flows on River Corridor and Watershed Biogeochemical Function

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Stegen

We are developing predictive understanding of the processes that govern influences of hydrologic exchange flows on water quality, nutrient dynamics, and ecosystem health in dynamic river corridor…

Microscopic visualization of membrane pores and bacterial foulants

Lead Institution

University of Houston

Principal Investigator

Shankar Chellam

Project type

Exploratory Research

My research is mainly centered on membrane technologies for municipal water purification. We (1) elucidate contaminant transport and membrane fouling mechanisms, (2) devise methods to enhance…

Coupling of Fe and Tc Speciation in Subsurface Sediments: Implications to Long-Term Tc Immobilization (Zachara NABIR-Fe/Tc, PNNL Scope # 40761)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

Project type

Exploratory Research

Creating an in-situ bio-barrier containing sediment-bound, biogenic FE(II) to reduce and collect mobile Tc(VII)O4 - The research objective is to provide scientific insights on the rates of reductive…

Carbon, Oxide, Metal Sequestration Mechanisms of Hot Springs Microbial Communities: Establishing a Technical Platform for Chemical Imaging Investigation

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sherry Cady

Though microbes can indirectly and metabolically transform, sequester, and transport (i) ions, (ii) organic and inorganic complexes, and (iii) precipitates via a wide range of mechanisms,…

Understanding the Complexity of Biogenic Mineral Formation in Microbial Extracellular Polymeric Substances: a 3-d Nano-scale Perspective

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Microbial role in sequestration of various metal has been well studied, including PNNL Microbiology group and numerous EMSL user projects. However, the current view of microbial-mineral interactions…

Biogenic Iron Materials

Lead Institution

J. Craig Venter Institute

Principal Investigator

Yuri Gorby

Project type

Exploratory Research

We will image iron minerals produced by the reduction of solid phase ferric oxides. The research will require the use of field emission SEM and TEM.

The Role of Microenvironments and Physicochemical Subsurface Components on Field Scale Biogeochemistry and Contaminant Mobility

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Exploratory Research

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Uranium Biomineralization by Caulobacter crescentus

Lead Institution

Lawrence Livermore National Laboratory

Principal Investigator

Yongqin Jiao

Project type

Large-Scale EMSL Research

The research goal of this proposal is to understand and evaluate the potential of using the aerobic bacterium Caulobacter crescentus for the application of uranium bioremediation. Previous work by…

Influences of Hydrologic Exchange Flows on River Corridor and Watershed Biogeochemical Function

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Stegen

We are developing predictive understanding of the processes that govern influences of hydrologic exchange flows on water quality, nutrient dynamics, and ecosystem health in dynamic river corridor…

EMSL Science Overview

Alice Dohnalkova

Projects

Novel flow based biomolecule immobilization on nanoporous scaffolds
for enhanced biosensor performance and drug delivery applications

Rhizosphere Underground:
Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

Influences of Hydrologic Exchange Flows on River Corridor and Watershed Biogeochemical Function

Microscopic visualization of membrane pores and bacterial foulants

Coupling of Fe and Tc Speciation in Subsurface Sediments: Implications to Long-Term Tc Immobilization (Zachara NABIR-Fe/Tc, PNNL Scope # 40761)

Carbon, Oxide, Metal Sequestration Mechanisms of Hot Springs Microbial Communities: Establishing a Technical Platform for Chemical Imaging Investigation

Understanding the Complexity of Biogenic Mineral Formation in Microbial Extracellular Polymeric Substances: a 3-d Nano-scale Perspective

Biogenic Iron Materials

The Role of Microenvironments and Physicochemical Subsurface Components on Field Scale Biogeochemistry and Contaminant Mobility

Uranium Biomineralization by Caulobacter crescentus

Influences of Hydrologic Exchange Flows on River Corridor and Watershed Biogeochemical Function