Alice Dohnalkova

Correlative High-Resolution Imaging and Spectroscopy to Characterize the Structure and Biogeochemical Function of Microbial Biofilms

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Matthew Marshall

Project type

Exploratory Research

This proposal leverages knowledge and experience gained from DOE-funded research to elucidate the mechanisms involved in dissimilatory metal reduction, as well as, microscale imaging and analytical…

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL.PNNL Scientific Focus Area. The PNNL SFA will develop a predictive understanding…

Extracellular Research Thrust for EMSL SSA

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kirsten Hofmockel

This project will develop a conceptual framework that defines the biotic and abiotic mechanisms regulating the persistence of mineral associated organic matter (OM). The specific goals are to…

Electron microscopy imaging of cyanobacteria Synechcystis sp.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Project type

Grand Challenge

As a part of Grand Challenges in Membrane biology, Synechocystis sp. will be investigated in regards of cell morphology and elemental analysis of minerals associated with their outer and inner…

Novel flow based biomolecule immobilization on nanoporous scaffolds
for enhanced biosensor performance and drug delivery applications

Lead Institution

University of Washington

Principal Investigator

Amy Shen

Project type

Exploratory Research

Biosensors play indispensible roles in disease diagnosis, drug screening, and forensic applications. One important area of biosensor research is the immobilization of biomolecules (i.e. enzyme, DNA)…

Rhizosphere Underground:
Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Microorganisms are fundamental to biogeochemical cycling, as microbial metabolism significantly contributes to the regulation of terrestrial carbon and nitrogen cycling. However, the impact of the…

Influences of Hydrologic Exchange Flows on River Corridor and Watershed Biogeochemical Function

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Stegen

We are developing predictive understanding of the processes that govern influences of hydrologic exchange flows on water quality, nutrient dynamics, and ecosystem health in dynamic river corridor…

Microscopic visualization of membrane pores and bacterial foulants

Lead Institution

University of Houston

Principal Investigator

Shankar Chellam

Project type

Exploratory Research

My research is mainly centered on membrane technologies for municipal water purification. We (1) elucidate contaminant transport and membrane fouling mechanisms, (2) devise methods to enhance…

Coupling of Fe and Tc Speciation in Subsurface Sediments: Implications to Long-Term Tc Immobilization (Zachara NABIR-Fe/Tc, PNNL Scope # 40761)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

Project type

Exploratory Research

Creating an in-situ bio-barrier containing sediment-bound, biogenic FE(II) to reduce and collect mobile Tc(VII)O4 - The research objective is to provide scientific insights on the rates of reductive…

Carbon, Oxide, Metal Sequestration Mechanisms of Hot Springs Microbial Communities: Establishing a Technical Platform for Chemical Imaging Investigation

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sherry Cady

Though microbes can indirectly and metabolically transform, sequester, and transport (i) ions, (ii) organic and inorganic complexes, and (iii) precipitates via a wide range of mechanisms,…

Understanding the Complexity of Biogenic Mineral Formation in Microbial Extracellular Polymeric Substances: a 3-d Nano-scale Perspective

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Microbial role in sequestration of various metal has been well studied, including PNNL Microbiology group and numerous EMSL user projects. However, the current view of microbial-mineral interactions…

EMSL Science Overview

Alice Dohnalkova

Projects

Correlative High-Resolution Imaging and Spectroscopy to Characterize the Structure and Biogeochemical Function of Microbial Biofilms

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone

Extracellular Research Thrust for EMSL SSA

Electron microscopy imaging of cyanobacteria Synechcystis sp.

Novel flow based biomolecule immobilization on nanoporous scaffolds
for enhanced biosensor performance and drug delivery applications

Rhizosphere Underground:
Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

Influences of Hydrologic Exchange Flows on River Corridor and Watershed Biogeochemical Function

Microscopic visualization of membrane pores and bacterial foulants

Coupling of Fe and Tc Speciation in Subsurface Sediments: Implications to Long-Term Tc Immobilization (Zachara NABIR-Fe/Tc, PNNL Scope # 40761)

Carbon, Oxide, Metal Sequestration Mechanisms of Hot Springs Microbial Communities: Establishing a Technical Platform for Chemical Imaging Investigation

Understanding the Complexity of Biogenic Mineral Formation in Microbial Extracellular Polymeric Substances: a 3-d Nano-scale Perspective