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Andrew Lipton

Institution
Environmental Molecular Sciences Laboratory

Projects

43Ca NMR Spectroscopy of Ca2+ Dependent Proteins and models

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Paul Ellis
Project type
Large-Scale EMSL Research
We are exploring the application and utility of low temperature solid-state NMR methods to the observation of 43Ca in Ca2+ dependent proteins. Our first application is to the protein calbindin D9k …

Investigation of the role of Mg2+ in DNA repair proteins APE1, Pol ?, and FEN1

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
Nuclear DNA is continuously damaged by endogenous sources, such as the reactive free radicals produced during oxidative metabolism, or by exogenous sources, such as ionizing radiation. It has been…

Extracellular Research Thrust for EMSL SSA

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Kirsten Hofmockel
This project will develop a conceptual framework that defines the biotic and abiotic mechanisms regulating the persistence of mineral associated organic matter (OM). The specific goals are to…

Investigation of the role of Mg2+ in DNA repair proteins APE1, Pol ?, and FEN1

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
Nuclear DNA is continuously damaged by endogenous sources, such as the reactive free radicals produced during oxidative metabolism, or by exogenous sources, such as ionizing radiation. It has been…

Investigation of the role of Mg2+ in DNA repair proteins APE1, Pol ?, and FEN1

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
Nuclear DNA is continuously damaged by endogenous sources, such as the reactive free radicals produced during oxidative metabolism, or by exogenous sources, such as ionizing radiation. It has been…

Investigation of the role of Mg2+ in DNA repair proteins APE1, Pol ?, and FEN1

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Paul Ellis
Project type
Capability Research
We propose to utilize unique capabilities within the EMSL (low temperature solid-state NMR and the mpp2 super computer) to investigate Mg2+ binding to two DNA repair proteins Pol ? and APE1. In the…

Probing the Dynamics of a Protein Hydrophobic Core at Low Temperatures

Lead Institution
University of Alaska at Anchorage
Principal Investigator
Liliya Vugmeyster
Project type
Exploratory Research
Hydrophobic cores are found in the interior of globular proteins and are essential for the formation of the folded state. The core represents a complex dynamical medium reflecting the existence of a…

Carriers for Transporting and Storing Hydrogen

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Thomas Autrey
This proposal will determine the mechanistic and thermodynamic understanding required to address bottlenecks in materials capable of storing or transporting hydrogen. We propose a series of…

The Mechanism of Action of Carbonic Anhydrase and LpxC

Lead Institution
University of Michigan
Principal Investigator
Carol Fierke
Project type
Capability Research
Zinc binding sites in proteins play diverse roles, including enhancement or inhibition of catalysis, stabilization and assembly of protein structures, and regulation of metal concentration. Further…

Invesitgation of the role of Mg2+ in DNA repair proteins

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
The chemistry central to the function of the DNA repair proteins APE1, Pol b and FEN1 is the chemistry of water activated by a magnesium ion. Further, Mg2+ also facilitates the organization of the…

Invesitgation of the role of Mg2+ in DNA repair proteins

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
The chemistry central to the function of the DNA repair proteins APE1, Pol b and FEN1 is the chemistry of water activated by a magnesium ion. Further, Mg2+ also facilitates the organization of the…

Magnesium NMR of DNA Repair Proteins

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Exploratory Research
The chemistry central to the function of the DNA repair proteins apurinic/apyrimidic endonuclease 1 (APE1) and polymerase beta (pol beta) is the chemistry of water activated by a magnesium ion. …

Analysis of zinc environments in singlet oxygen-responsive proteins

Lead Institution
University of Wisconsin, Madison
Principal Investigator
Timothy Donohue
Project type
Capability Research
This project will use solid state NMR to probe the binding and environment of zinc ligands in the ChrR protein from the photosynthetic bacterium Rhodobacter sphaeroides. ChrR is a zincmetalloprotein…

Metal Binding by Melanin Biopolymers

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Eric Walter
The ubiquitous biopolymer melanin has important and unique materials properties, yet these very properties have prevented elucidation of its basic structure. MAS-NMR capabilities at EMSL will probe…

Solid-State 67Zn NMR of Synthetic Metalloprotein Models

Lead Institution
Columbia University
Principal Investigator
Gerard Parkin
Project type
Capability Research
It is the invention of this proposal to describe studies that will be undertaken in order to aid our understanding of the structures and mechanisms of zinc enzymes. Such knowledge will be obtained…

Advancing soil C characterization using HRMS and NMR

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Nancy Hess
Soil organic matter consists of diverse and complex mixture of hundreds to thousands biological and metabolic products that are poorly characterized using current techniques. We seek to develop a…