Andrey Liyu

Spatially-resolved proteome mapping of senescent cells and their tissue microenvironment at single-cell resolution

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Cellular senescence is a permanent state of cell cycle arrest induced by many different stresses. Although senescent cells (SNCs) have been demonstrated with beneficial roles in normal physiological…

Deep UV laser ablation system for spatial single-cell proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

William Chrisler

Laser capture microdissection (LCM) is the key capability for spatially resolved omicss. However, commercially available LCM systems are designed for mammalian tissues and feature UV lasers (330 nm),…

Building an automation-ready multimodal workflow for comprehensive 3D characterization of intact soil structures.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Odeta Qafoku

3-dimensional (3D) characterization of intact soil structures (i.e. macro and microaggregates) will provide understandings on how soil physical, mineralogical, biotic heterogeneities control long…

Dynamics of Supported Noble metal Nanoparticles in the Presence of Oxidizing Environment: Application of Compressive Sensing in ETEM

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Libor Kovarik

Direct atomic level visualization of structural and surface transformations of nanoparticles during exposure to oxidizing environment is critical for developing a structure-property relationship in…

Advancement of the molecular microscope to enable high-resolution dynamic imaging and unprecedented spatial metabolomic information of living systems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

The ability to capture and visualize the complex metabolic behavior of living organisms in situ is a bioanalytical grand challenge that has yet to be fully realized. Ambient ionization mass…

Nanoliter-scale Autosampler for Seamless Integration of NanoPOTS Platform with LC-MS

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ying Zhu

We propose to develop a high throughput, automated, and ultrasensitive single cell proteomic platform by seamlessly integrating our nanodroplet Processing in One-pot for Trace Sample technique with…

Targeted spatial metabolomics: an automated approach to identifying and analyzing single cells in situ

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Tissue-scale or cell-population-level analysis (i.e., ‘bulk analysis’) yields averaged abundance data that can mask critical information about the state of individual cells. Through a recently ended…

High throughput and in-depth single-cell proteomics for plant cells

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ying Zhu

In support of the "Single-cell biology" value stream in EMSD/EMSL DigiPhen Motivation, we propose to develop a high throughput and in-depth single-cell proteomics platform. We aim to…

An EMSL User-ready spatially resolved metabolomics platform capable of high-lateral MS imaging and epifluorescence-based targeting

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

To upgrade our laser-ablation electrospray ionization mass spectrometry (LAESI-MS) platform to a capability that can automatically identify cells and areas of interest and acquire spatially resolved…

EMSL Science Overview

Andrey Liyu

Projects

Spatially-resolved proteome mapping of senescent cells and their tissue microenvironment at single-cell resolution

Deep UV laser ablation system for spatial single-cell proteomics

Building an automation-ready multimodal workflow for comprehensive 3D characterization of intact soil structures.

Dynamics of Supported Noble metal Nanoparticles in the Presence of Oxidizing Environment: Application of Compressive Sensing in ETEM

Advancement of the molecular microscope to enable high-resolution dynamic imaging and unprecedented spatial metabolomic information of living systems

Nanoliter-scale Autosampler for Seamless Integration of NanoPOTS Platform with LC-MS

Targeted spatial metabolomics: an automated approach to identifying and analyzing single cells in situ

High throughput and in-depth single-cell proteomics for plant cells

An EMSL User-ready spatially resolved metabolomics platform capable of high-lateral MS imaging and epifluorescence-based targeting