Brian O'callahan

Infrared Vibrational Nano-Spectroscopy: Pushing Sensitivity and Application Space for Bio-imaging

Lead Institution

University of Colorado at Boulder

Principal Investigator

Bernd Raschke

We request access to the scattering scanning near-field optical microscope (s-SNOM) we have developed during a partner proposal, and scanning electron microscope / focused ion beam (SEM/FIB) facility…

Multimodal Nanoscale Chemical Imaging of Intra- and Inter-Cellular Biomolecules Involved in Microbial Interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Lea

Microbes orchestrate a suite of intra- and inter-cellular biomolecules to carry out diverse metabolic processes. Existing genomic and biochemical methods cannot directly probe the physical…

Multimodal Nanoscale Chemical Imaging of Intra- and Inter-Cellular Biomolecules Involved in Microbial Interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Lea

Microbes orchestrate a suite of intra- and inter-cellular biomolecules to carry out diverse metabolic processes. Existing genomic and biochemical methods cannot directly probe the physical…

Multimodal Chemical Imaging Across Scales to Visualize Metabolic Pathways in Live Plants and Microbial Systems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Lea

We will develop a novel spectral imaging platform (“BioImager”) that may be used to image and identify biomolecules involved in both microbial and plant metabolic processes through different chemical…

Discover ectomycorrhizal fungi-triggered macro- and micronutrient reactions and movements: From cell to ecosystem function

Lead Institution

University of Florida

Principal Investigator

Hui-ling Liao

Project type

FICUS Research

As the critical members of forest microbiome, ectomycorrhizal fungi (EMF) play important roles in forest ecosystems. EMF provide their tree hosts with macro- and micronutrients in exchange for…

Mineralogy exerts control on organic molecules zonal structuring and reordering

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Odeta Qafoku

Organic C preservation in soil environments is predominantly driven by organic matter: soil mineral interactions. Yet, mechanistic uncertainties remain, namely how spatial variation in mineralogy…

Flow cell with environmental control for in-liquid nano-FTIR

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Brian O'callahan

Total internal reflection scattering-scanning near-field optical microscopy (TIR s-SNOM) has achieved chemical nano-imaging in liquid, however of only static systems. In this project, a flow cell…

Harnessing Biomineralization Potential of Metal-Oxidizing Bacteria for REE Synthesis

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alex Beliaev

Although redox reactions and enzymes participating in the extracellular oxidation of Fe and Mn are identified, little is known about the biomineralization mechanisms that facilitate formation…

Multimodal Chemical Imaging Across Scales to Visualize Metabolic Pathways in Live Plants and Microbial Systems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Lea

We will develop a novel spectral imaging platform (“BioImager”) that may be used to image and identify biomolecules involved in both microbial and plant metabolic processes through different chemical…

EMSL Science Overview

Brian O'callahan

Projects

Infrared Vibrational Nano-Spectroscopy: Pushing Sensitivity and Application Space for Bio-imaging

Multimodal Nanoscale Chemical Imaging of Intra- and Inter-Cellular Biomolecules Involved in Microbial Interactions

Multimodal Nanoscale Chemical Imaging of Intra- and Inter-Cellular Biomolecules Involved in Microbial Interactions

Multimodal Chemical Imaging Across Scales to Visualize Metabolic Pathways in Live Plants and Microbial Systems

Discover ectomycorrhizal fungi-triggered macro- and micronutrient reactions and movements: From cell to ecosystem function

Mineralogy exerts control on organic molecules zonal structuring and reordering

Flow cell with environmental control for in-liquid nano-FTIR

Harnessing Biomineralization Potential of Metal-Oxidizing Bacteria for REE Synthesis

Multimodal Chemical Imaging Across Scales to Visualize Metabolic Pathways in Live Plants and Microbial Systems