Christopher Anderton

Spatial Metabolomics and Glycomics for Human Kidneys

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Acute kidney injury (AKI) and chronic kidney disease (CKD) are major contributors to overall morbidity and mortality in patients in the US. However, we lack a clear understanding of the major…

Iron, Ferroptosis and Ovarian Cancer

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

The goal of this proposal is to provide mechanistic information on the role of iron in ferroptosis, test how manipulating pathways of iron and lipid metabolism impact ferroptosis, and explore how…

Next-generation stimulated Raman scattering (SRS) microscopy to overcome photodamage and resolution limitations for real-time tracking of lipid/carbohydrate interfaces in plants, algae, and fungi

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

We will enhance the sensitivity through quantum- enhanced noise suppression in the SRS signal. The enhanced sensitivity will benefit from lower excitation power, lower detection concentrations, and…

Super resolution fluorescence imaging of plant receptors

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Dehong Hu

This proposal covers work done under a DOE BER funded research project led by the University of Missouri. The project focuses on developing and demonstrating the application of a fluorescence…

Multi-Scale Multi-Modality High Resolution Mapping of the Adult Human Lung

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

The Human Biomolecular Atlas Program (HuBMAP) is entering the production phase, with an emphasis on progressive, high sensitivity and specificity assays, prioritizing spatial biomolecular analysis…

A 3D printed SubTap platform for high-throughput plant-microbe interaction studies

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Arunima Bhattacharjee

High-throughput (HTP) mass spectrometry will provide a holistic analysis of microbial and plant community interactions. The recently developed 3D printed SubTap platform (through a collaborative work…

Development of nanospray desorption electrospray ionization with sample imprinting as a tool to characterize microbial and below-ground interfaces

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Mass spectrometry imaging (MSI) is a well-established strategy to observe spatial molecular-level information. However, the vacuum associated with many imaging modalities do not allow for the…

Innovative Molecular Imaging Using Spatially Controlled Ionization Combined with High Performance FT-ICR Mass Spectrometry

Lead Institution

Maastricht University

Principal Investigator

Ronald Heeren

Project type

Scientific Partner

The objective of this proposed 3 year EMSL-FOM institute AMOLF partnership is to incorporate C60 SIMS and MALDI ionization techniques with a 9.4 Tesla FT-ICR mass spectrometer to enable and enhance…

Tracking the Fate of new C in Northern Peatlands by a Compound-Specific Stable Isotope-Labeling Approach coupled with FISH-NanoSIMS

Lead Institution

University of Arizona

Principal Investigator

Malak Tfaily

Here, we propose to examine the priming effect, drivers, and dynamics of the "new C" that is stimulated by climate change in Northern peatlands soil using a stable isotope labeling approach…

Development and implementation of an in situ high-resolution isotopic microscope for measuring metabolic interactions in soil mesocosms

Lead Institution

University of Massachusetts Medical School

Principal Investigator

Elizabeth Shank

Soil microbial processes exert a major influence on many of our planet's ecosystems, yet our ability to directly observe the enzymatic and metabolic activities of microbes within soil is…

Genome-enabled Investigations of the Role of Secreted Proteins and Reactive Metabolites in Carbon Degradation by Pure and Mixed Ascomycete Fungal Communities

Lead Institution

Woods Hole Oceanographic Institution

Principal Investigator

Colleen Hansel

Project type

FICUS Research

The Eumycete fungi (primarily Basidiomycota and Ascomycota) are key organisms in the degradation of wood, plant and animal material. While ascomycetes are less capable of rapid wood decay than…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

Advancement of the molecular microscope to enable high-resolution dynamic imaging and unprecedented spatial metabolomic information of living systems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

The ability to capture and visualize the complex metabolic behavior of living organisms in situ is a bioanalytical grand challenge that has yet to be fully realized. Ambient ionization mass…

Quantifying the functional and structural states of dynamic microbial communities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Fredrickson

Project type

Large-Scale EMSL Research

The overall goal of this proposal is to provide a quantitative framework in which to compare species-resolved patterns of gene expression, allowing comparison of the effects of perturbations of…

Carbon Sequestration Beneath the Active Zone of the Rhizosphere: Insights from Extremophile Ecosystems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sherry Cady

To better understand the ultimate fate of carbon sequestered in the subsurface, we are studying carbon sequestration mechanisms in mineralizing extremophile environments. Such ecosystems provide an…

Targeted spatial metabolomics: an automated approach to identifying and analyzing single cells in situ

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Tissue-scale or cell-population-level analysis (i.e., ‘bulk analysis’) yields averaged abundance data that can mask critical information about the state of individual cells. Through a recently ended…

Multi-Omics Evaluation of Tripartite Associations in Red Alder Roots: Integrated Transcriptomics, Proteomics, Metabolomics and Metabolite Imaging of Red Alder Symbiotic Associations in Root Tissue

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

This proposal presents the unique opportunity to conduct the first ever integrated multi-omics and imaging evaluation of the Alnus rubra (red alder tree)/Frankia alni (bacteria)/Alpova diplophloeus …

Characterizing the chemical heterogeneity of native urban films

Lead Institution

University of Iowa

Principal Investigator

Scott Shaw

Project type

Limited Scope

Environmental surface films (urban films) are ubiquitous coatings on exterior surfaces that form as particulate or molecular biogenic and anthropogenic emissions deposit from the atmosphere onto…

Development and implementation of an in situ high-resolution isotopic microscope for measuring metabolic interactions in soil mesocosms

Lead Institution

University of Massachusetts Medical School

Principal Investigator

Elizabeth Shank

Soil microbial processes exert a major influence on many of our planet's ecosystems, yet our ability to directly observe the enzymatic and metabolic activities of microbes within soil is…

Biomineralization and Biosequestration Mechanisms at Life's Upper Temperatures: A Reappraisal using Novel CryoEM Techniques

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sherry Cady

Though microbes can indirectly and metabolically transform, sequester, and transport (i) ions, (ii) organic and inorganic complexes, and (iii) precipitates via a wide range of mechanisms,…

PhenoAccess: Physiological Phenotyping of Brachypodium Accessions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

Although the effects of elevated CO2 on plant growth, physiology and metabolism has been investigated thoroughly, the underlying integrated organismal, cellular and molecular mechanisms of these…

Multiplexed high resolution fluorescence imaging in plants

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

This proposal covers work done under a DOE-funded project led by the University of Missouri. The project focuses on developing and demonstrating the application of a fluorescence microscopy platform,…

Multimodal Imaging and chemical analysis of Sea Spray Aerosols

Lead Institution

University of California, San Diego

Principal Investigator

Nathan Gianneschi

Project type

Special Science

The marine environment is a highly diverse and complex system, playing an important role in biogeochemical cycling of the world carbon stock. Sea spray aerosols (SSA) make significant contributions…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

Prediction of soil microbiome phenotypic responses to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

Carbon, Oxide, Metal Sequestration Mechanisms of Hot Springs Microbial Communities: Establishing a Technical Platform for Chemical Imaging Investigation

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sherry Cady

Though microbes can indirectly and metabolically transform, sequester, and transport (i) ions, (ii) organic and inorganic complexes, and (iii) precipitates via a wide range of mechanisms,…

Spatial Metabolomics for Human Kidneys

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Acute kidney injury (AKI) and chronic kidney disease (CKD) are major contributors to overall morbidity and mortality in patients in the US. However, we lack a clear understanding of the major…

SyPro Poplar: Improving Poplar Biomass Production under Abiotic Stress Conditions: an
Integrated Omics, Bioinformatics, Synthetic Biology and Genetic Engineering Approach

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

To engineer bioenergy crops that can grow in dry, marginal lands, this project will introduce into poplar trees tolerance to different environmental stresses such as water deficit, high soil salinity…

New Mesoscale Multi-Modal Imaging Of Cellular Communication Between Microbe And Plant In The Rhizosphere – A Pilot Project

Lead Institution

University of Missouri - Columbia

Principal Investigator

Richard Ferrieri

The interaction between plants and their environment is of key importance in promoting sustainable, healthy, and robust plant growth. Underlying the ability of plants to cope with a variety of…

Combining surface analysis and mass spectrometry methods to expand the information available from biologically and environmentally relevant interfaces

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Donald Baer

EMSL and the NESAC/BIO (National Electron Spectroscopy Analysis Center for Biology) at the University of Washington are world leaders in the application of surface analysis and mass spectrometry to a…

Spatial Metabolomics and Glycomics for Human Kidneys

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Acute kidney injury (AKI) and chronic kidney disease (CKD) are major contributors to overall morbidity and mortality in patients in the US. However, we lack a clear understanding of the major…

Control of methane and carbon dioxide emissions by sulfur biogeochemistry in terrestrial ecosystems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

John Cliff

Project type

Large-Scale EMSL Research

Freshwater wetlands are responsible for 40% of the methane emissions worldwide. Despite very low amounts of ambient sulfate, these ecosystems often support high rates of sulfate reduction, rivaling…

MS imaging of cell wall N- and O-linked glycans in interaction between roots and soil-borne microbes

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

We propose to address a critical barrier in deciphering rhizosphere communication, specifically that of the role of glycans (a modifiable moiety of glycoproteins) in these processes. The first aim of…

Beam ionization: who gets the charge and why do some get broken up about it?

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Beam ionization is the key method for directly probing samples for in situ chemical analysis. We aim to understand the mechanisms of small molecule desorption and ionization from different substrates…

Development of an in situ multimodal mass spectrometry imaging approach for studying the rhizosphere: from revealing activation of metabolic pathways to elucidating nanoscale exchanges

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Gaining spatial information of biochemical interactions that occur within the rhizosphere and other subsurface terrestrial ecosystems is an area of major interest. Through bulk extraction methods, we…

Prediction of soil microbiome phenotypic responses to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

Iron, Ferroptosis and Ovarian Cancer

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

The goal of this proposal is to provide mechanistic information on the role of iron in ferroptosis, test how manipulating pathways of iron and lipid metabolism impact ferroptosis, and explore how…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

SyPro Poplar: Improving Poplar Biomass Production under Abiotic Stress Conditions: an
Integrated Omics, Bioinformatics, Synthetic Biology and Genetic Engineering Approach

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

To engineer bioenergy crops that can grow in dry, marginal lands, this project will introduce into poplar trees tolerance to different environmental stresses such as water deficit, high soil salinity…

An EMSL User-ready spatially resolved metabolomics platform capable of high-lateral MS imaging and epifluorescence-based targeting

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

To upgrade our laser-ablation electrospray ionization mass spectrometry (LAESI-MS) platform to a capability that can automatically identify cells and areas of interest and acquire spatially resolved…

New Mesoscale Multi-Modal Imaging Of Cellular Communication Between Microbe And Plant In The Rhizosphere -- A Pilot Project

Lead Institution

University of Missouri - Columbia

Principal Investigator

Richard Ferrieri

The interaction between plants and their environment is of key importance in promoting sustainable, healthy, and robust plant growth. Underlying the ability of plants to cope with a variety of…

Utilizing LESA to characterize cell-to-cell communication through the secreted metabolomes of microbial communities

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Inter-cellular communication is a complex process that plays a pivotal role in the microbiome and is essential for our understanding of microbial dynamics and functional roles. Teasing apart…

Development and implementation of an in situ high-resolution isotopic microscope for measuring metabolic interactions in soil microcosms

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Soil microbial processes exert a major influence on many of our planet's ecosystems, yet our ability to directly observe the enzymatic and metabolic activities of microbes within soil is…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

Reprogramming Plant Gene Regulatory Networks for Source-to-Sink Carbon Allocation

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

The regulation of carbon partitioning between source and sink tissues plays a critical role in plant growth and development. Plant productivity is enhanced by sink strength and source activity, which…

Toward plant spatial hormonomics: multiple phytohormone profiling by derivatization assisted-ionization enhanced targeted MALDI MS imaging

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Dusan Velickovic

Phytohormones are physiologically important small molecules that regulate diverse processes as chemical messengers within plants and in plant communications with environments. However, the complex…

Probing the Origins of Carbonate Precipitation by In Situ Nanoscale Microbe-Mineral Analysis and Mapping

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sherry Cady

Calcium carbonate (CaCO3) precipitation through geological time has sequestered large amounts of CO2, but identification of non-skeletal CaCO3, especially bacterial, precipitates is often difficult…

A Molecular Atlas of the Developing Lung

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our understanding of alveologenesis is a critical step…

Soil-like habitats for spatial analysis of microbe-mineral interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Arunima Bhattacharjee

The objective of this study is to develop mineral microfluidics compatible with a suite of optical and chemical imaging capabilities at EMSL, as well as SSRL, for probing microbially relevant…

Molecular Mechanism of PGPR-Plant Associations

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

Project type

Large-Scale EMSL Research

A large body of literature attests to the plant growth promotion effects of various endophytic bacteria, including significant increases in crop yield. These studies include the possible impact of…

Projects