Daniel Kohl

Washington University in St. Louis

Projects

From Activity to ORF

Lead Institution

Washington University in St. Louis

Principal Investigator

Daniel Kohl

Project type

Exploratory Research

THE PROBLEM: There are many known activities, the genes for which are unknown Although vast databases of DNA sequences are now available for many organisms, there are still many known protein…

Using Mass Spectrometry to Assist in Identifying Genes for Enzymes of Known Activity, Unknown OpenReadingFrame and Small Abundance

Lead Institution

Washington University in St. Louis

Principal Investigator

Daniel Kohl

Project type

Exploratory Research

We have reported the identification of the Open Reading Frame (ORF) that expresses a NADPH-dependent methylglyoxal reducing enzyme based on the partial purification of this activity through 3 simple…