David Koppenaal

Investigation of protein degradation mechanisms in Synechococcus elongates UTEX 2973

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

The recently identified and characterized unicellular cyanobacteria, Synechococcus elongates UTEX 2973, a fast growing and genetically malleable organism, has the potential to become the definitive…

Cyanobacteria Membrane Biology Grand Challenge: Systems Analysis of the Dynamics of Membrane Architecture, Composition, and Function- Proteomic, Metabolomic, and Metallomic Characterization

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Project type

Grand Challenge

The strain Cyanothece sp. ATCC 51142, isolated from benthic waters off the coast near Galveston, Texas, is a unicellular strain approximately 4-7 &#956;m in diameter along the major axis. This…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

LC-MS-based metabolomics for computational toxicology

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

The goal of this project is to assist the Environmental Protection Agency in analyzing samples from a fish-model of environmental exposure. LC-MS-based metabolomics will be utilized to help develop…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

Sensitive Detection of Protein Phosphorylation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Charles Barinaga

Project type

Exploratory Research

EHSD (FS) LDRDThe objective is to demonstrate the applicability of ICP/MS to the sensitive detection and quantitation of phosphorylation on peptides and proteins. The scope is to develop sufficient…

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

High-Throughput Proteomics: Applications and Development (PNNL Scope #40601 and #43488)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Gordon Anderson

This project involves the application and development of high-throughput proteomics and quantitative measurement capabilities within biological systems. More so, application measurements will be…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

Innovative Molecular Imaging Using Spatially Controlled Ionization Combined with High Performance FT-ICR Mass Spectrometry

Lead Institution

Maastricht University

Principal Investigator

Ronald Heeren

Project type

Scientific Partner

The objective of this proposed 3 year EMSL-FOM institute AMOLF partnership is to incorporate C60 SIMS and MALDI ionization techniques with a 9.4 Tesla FT-ICR mass spectrometer to enable and enhance…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

Liquid Sampling-Atmospheric Pressure Glow Discharge (LS-APGD) Ionization Source for Elemental Analysis

Lead Institution

Clemson University

Principal Investigator

Richard Marcus

Project type

Exploratory Research

The development of a novel low power, low flow 'elemental' ionization source for applications in the areas of metallomics and transportable mass spectrometry is proposed. The liquid…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Membrane Biology Research Campaign: Use of synthetic biology to probe molecular machines in photosynthesis

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

Project type

Research Campaign

We have shown in our previous EMSL collaborations our ability to develop systems-level models of cyanobacterial processes. In the present proposal, our objective is to characterize and…

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Fourier transform ion cyclotron mass spectrometry (FTICR-MS), the highest performance mass spectrometry technique, is uniquely valued as a research tool for analytical applications requiring the…

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

Development and refinement of an in situ 'molecular microscope' utilizing ultrahigh resolution mass spectrometry

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

The ability to elucidate complex metabolic behavior of living organisms in situ and with cellular resolution is a bioanalytical grand challenge that is yet to be realized. Mass spectrometry imaging …

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Projects