David Panther

Nanoscale Characterization of Nanomaterial - Cell Interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

Project type

Large-Scale EMSL Research

This proposal is a continuation of research pursued under EMSL User Proposal #17690. The extensive use of amorphous silica nanoparticles (NPs) in the industry, and the growing use of carbon nanotubes…

Single Molecule Approach for Understanding EGFR Molecular Interactions (Orr LDRD-FRET, PNNL Scope #48287)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

Project type

Exploratory Research

We will use cutting edge fluorescence imaging techniques to study the molecular interactions of EGFR with other molecules in the pathway to better understand its role in determining cell fate.

Dynamic Changes in Molecular Interactions along the Circadian Rhythm (Orr-LDRD Membrane Biology GC, PNNL Scope #90001)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

Project type

Grand Challenge

One of the most challenging tasks in cell biology is the study of the molecular interactions in the natural cellular environment in order to gain information about their dynamic changes in time and…

Nanoscale Insight into the Living Cell Membrane Responses to Ultrafine PM (Orr LDRD-EBI, PNNL Scope #49989)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

Project type

Exploratory Research

Ultrafine particulate air pollutants exert harmful effects on human health to a greater extent than other fine particulate matter (PM), and these effects have been linked to the surface properties of…

FRET Screening of Protein-Protein Interactions in Shewanella (Orr GTL-Imaging, PNNL/ORNL Scope #43930)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

Project type

Exploratory Research

Fluorescence resonance energy transfer (FRET) imaging and spectroscopy techniques will be used to identify specific molecular interactions among proteins in Shewanella. The techniques incorporate…

Regulation of Cell Surface Ligand Dynamics (Orr LDRD-BSI, PNNL Scope #90001)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

Project type

Exploratory Research

We will develop a set of new imaging technologies that can directly visualize ligand oligomerization as well as interaction with other cell surface molecules. We expect to observe an increase in…

Roles for Membrane Receptors in Cellular Responses to External Cues;
The Case of ErbB4 and Morphology Dynamics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

Project type

Exploratory Research

Understanding the mechanisms that underlie the cellular responses to the environment has been the subject of intensive research. Membrane proteins are found within the interface between the living…

EMSL Science Overview

David Panther

Projects

Nanoscale Characterization of Nanomaterial - Cell Interactions

Single Molecule Approach for Understanding EGFR Molecular Interactions (Orr LDRD-FRET, PNNL Scope #48287)

Dynamic Changes in Molecular Interactions along the Circadian Rhythm (Orr-LDRD Membrane Biology GC, PNNL Scope #90001)

Nanoscale Insight into the Living Cell Membrane Responses to Ultrafine PM (Orr LDRD-EBI, PNNL Scope #49989)

FRET Screening of Protein-Protein Interactions in Shewanella (Orr GTL-Imaging, PNNL/ORNL Scope #43930)

Regulation of Cell Surface Ligand Dynamics (Orr LDRD-BSI, PNNL Scope #90001)

Roles for Membrane Receptors in Cellular Responses to External Cues;
The Case of ErbB4 and Morphology Dynamics