Galya Orr

Computer Simulations of Protein-Nanomaterial Interactions

Lead Institution

Universidade Federal de Pernambuco

Principal Investigator

Roberto Lins Neto

Project type

Capability Research

The development of nanotechnology has led to an increasing interest in interfacing biological molecules with nanomaterials. Proteins have been used to functionalize nanomaterials controlling their…

Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Increase the throughput for quantifying transcript copies in single microbes using single molecule fluorescence in situ hybridization

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Dehong Hu

Single molecule fluorescence in situ hybridization (smFISH) is a powerful method to provide information about the spatial location, species, and activities of microbes through their gene expression…

Fluorescence Imaging of Cellular Protein Dynamics

Lead Institution

Brigham Young University

Principal Investigator

Gretchen Carr

Project type

Exploratory Research

Advance fluorescence advanced technology will be used to study the interactions of proteins at the cell membrane and within the cytoplasm to better understand the molecular cascades underlying…

Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics to establish baseline cell biological and metabolite data on lipid production by the W29 strain of Yarrowia…

Single Cell Resolution Omics Analysis of T1D islets

Lead Institution

National Institutes of Health

Principal Investigator

Charles Ansong

Type 1 diabetes mellitus (T1D) is a chronic disease resulting from the autoimmune destruction of insulin-producing pancreatic beta cells. In addition to evolving ideas about the role of beta cells in…

Isoprene Research Campaign: Making Isoprene from Biomass Material using Bacillus Species

Lead Institution

Washington State University Tri-Cities

Principal Investigator

Birgitte Ahring

Project type

Research Campaign

The overarching goal of this project is to understand the pathway of isoprene production in Bacillus subtilis, to serve as the basis for genetically modifying the pathway to substantially increase…

Microbial Community Dynamics and Plant Phenomics with Single-Cell Gene Expression and Imaging Mass Spectrometry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

The inherent heterogeneity of biological systems, including microbial communities or cell populations, limits the questions that can be resolved by averaged cell population measurements, which lump…

Molecular Mechanisms Underlying Nanomaterial Interactions with Biological Systems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

This proposal covers research conducted with funding from several agencies, including NIEHS and the NSF-funded Center for Sustainable Nanotechnology. Accumulating observations demonstrate the…

EMSL Science Overview

Galya Orr

Projects

Computer Simulations of Protein-Nanomaterial Interactions

Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework

Increase the throughput for quantifying transcript copies in single microbes using single molecule fluorescence in situ hybridization

Fluorescence Imaging of Cellular Protein Dynamics

Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models

Single Cell Resolution Omics Analysis of T1D islets

Isoprene Research Campaign: Making Isoprene from Biomass Material using Bacillus Species

Microbial Community Dynamics and Plant Phenomics with Single-Cell Gene Expression and Imaging Mass Spectrometry

Molecular Mechanisms Underlying Nanomaterial Interactions with Biological Systems