Garry Buchko

Elucidating the essential molecular interactions for nucleation of calcite by designer biological and bio-mimetic materials.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Wendy Shaw

Project type

Contracted Time

Organisms produce hierarchically structured materials such as teeth, bones and skeletons through a process known as biomineralization. While the exact molecular details of the organic-inorganic…

Seattle Structural Genomics Center for Infectious Diseases – Year XVI

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The rapid spread of SARS-CoV-2 through-out the world and its life-changing impact on the day-to-day lives of Americans punctuate the need to be ever cautious of the emergence of new epidemic…

features guiding amelogenin’s transformation of calcium phosphate into enamel – FY23.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

One of nature’s hardest materials is enamel, which is 95% (by weight) carbonated hydroxyapatite (HAP). The exceptional functional properties of enamel arise from its intricate hierarchical structure…

Grand Challenge in Membrane Biology

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

Project type

Grand Challenge

In this grand challenge, we will use a systems approach to understand the network of genes and proteins that govern the structure and function of membranes and their components responsible for…

Screening protein crystals for XRD data collection at synchrotrons

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Limited Scope

To obtain a diffraction quality crystal it is often necessary to grow many crystals, mount, freeze, and then "shoot" them to see how well they diffract. Sometimes it may take 100s of…

Seattle Structural Genomics Center for Infectious Diseases --Year V

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Exploratory Research

The Seattle Structural Genomics Center for Infectious Disease (SSGCID) is funded by the National Institute of Allergy and Infectious Diseases (NIAID) to apply genome-scale approaches in solving…

Enamel biomineralization: A multi-disciplined approach to understand a natural protein-mineral interface. Year 3.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Aamelogenin is the central protein controlling the formation of dental enamel, the hardest tissue in the human body. It orchestrates the nucleation, growth, and organization of enamel (amelogenesis)…

Structural Biology of the Human High Mobility Group A (HMGA) Proteins

Lead Institution

Washington State University

Principal Investigator

Raymond Reeves

Project type

Capability Research

HMGA1 proteins play a critical role in the adaptive immune response to infectious microorganisms and viruses, affecting gene regulation that stimulates resting lymphocytes into active T-cells by…

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

Lead Institution

Washington State University

Principal Investigator

Raymond Reeves

Project type

Capability Research

Use MS to identify sites and types of post-translational modifications of several high mobility grop proteins. Use various tandem mass spec instruments available to precisely identify specific…

Seattle Structural Genomics Center for Infectious Diseases

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Exploratory Research

The Seattle Structural Genomics Center for Infectious Disease (SSGCID) is a consortium of four institutions (Seattle Biomedical Research Institue (SBRI), deCODE biostructures, University of…

Solution Structure of a Novel Anti-fungal Defensin, MtDEF4, from Medicago truncatula (barrel clover)

Lead Institution

Donald Danforth Plant Science Center

Principal Investigator

Thomas Smith

Project type

Limited Scope

The short-term goal of this proposal is to understand the modes of action of naturally occurring, potent, anti-fungal proteins called defensins. The long-term goal is to leverage these findings to…

Seattle Structural Genomics Center for Infectious Diseases – Year XII

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

A potential consequence of rapid climate change is the emergence of epidemic infectious zoonotic diseases as the habitats of disease carriers change to expose human populations to new infectious…

Structural Genomics: Determining the Structure of Proteins from the Infectious Agent Pseudomonas aeruginosa.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Capability Research

The antibiotic resistance of Pseudomonas aeruginosa in chronic infections of the lungs of Cystic Fibrosis (CF) patients remains an important health issue to more than 30,000 children and adults in…

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

Lead Institution

Washington State University

Principal Investigator

Raymond Reeves

Project type

Capability Research

The objective of this project is to provide new insight into the structural basis for some of the many biological processes involving the mammalian HMGA1 family of "high mobility group" …

Biasing conformational mobility of unstructured proteins by macromolecular crowding

Lead Institution

University of Idaho

Principal Investigator

Gary Daughdrill

Project type

Limited Scope

Macromolecular crowding is an entropic, excluded volume effect that occurs in the presence of inert macromolecules and has both thermodynamic and hydrodynamic consequences. The primary thermodynamic…

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Develop and apply new "Systems Biology" approaches to the study of bacterial pathogen species Salmonella and Yersinia, this includes proteomics, transcriptomics, metabolomics, lipidomics,…

Solid- and solution-state NMR approaches to characterizing the structure of amelogenin.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Biomineralization is the process used by living organism to produce minerals with properties not always found in their equivalent inorganically produced counterparts. In vertebrates, mineralized…

Structural Biology of the Human High Mobility Group A (HMGA) Proteins: Characterizing the Hub of Nuclear Function

Lead Institution

Washington State University

Principal Investigator

Raymond Reeves

Project type

Capability Research

The objective of this project is to provide new insight into the structural basis for some of the many biological processes involving the mammalian HMGA1 family of "high mobility group" …

A Systems Biology Approach to Infectious Diseases Research for EnteroPathogens

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Develop and apply new "Systems Biology" approaches to the study of bacterial pathogen species Salmonella and Yersinia, this includes proteomics, transcriptomics, metabolomics, lipidomics,…

Understanding structure-function relationships for developing catalysts for energy storage and production reactions.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Wendy Shaw

Project type

Large-Scale EMSL Research

The DOE Basic Energy Sciences Energy Frontier Research Center entitled “Center for Molecular Electrocatalysis” (Bullock), the DOE Basic Energy Sciences “Modular Energy-Based Approach to Molecular…

Solution-state NMR studies of catalytic intermediates of [Fe-Fe]-hydrogenase.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The goal of this research proposal is to establish concrete design principles that define and control efficient proton (H+) transport for [FeFe]-hydrogenase (H2ase). Proteins are able to perform…

Structural Studies of a Novel Family of Manganese Uptake Proteins in Cyanothece Containing a Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

Manganese is an essential trace element that all organisms require. Often, this metal is found in the enzyme?s active site, such as in ribonucleotide reductase, manganese-catalase, and manganese…

Structural Biology of DNA Repair Proteins: The Nudix Protein Family from the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans.

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Stephen Holbrook

Project type

Capability Research

The bacterium Deinococcus radiodurans is able to withstand high doses of ionizing and UV radiation that are lethal to virtually all other organisms (Battista, 1997). It has been suggested that this…

Seattle Structural Genomics Center for Infectious Diseases - Year VI

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Exploratory Research

The Seattle Structural Genomics Center for Infectious Disease (SSGCID) is funded by the National Institute of Allergy and Infectious Diseases (NIAID) to apply genome-scale approaches in solving…

Seattle Structural Genomics Center for Infectious Diseases – Year XIII

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

A potential consequence of rapid climate change is the emergence of epidemic infectious zoonotic diseases as the habitats of disease carriers change to expose human populations to new infectious…

Structural Studies of a Family of Proteins from the Diurnal Cyanobacteria Cyanothece 51142 that Contain an Unusual Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

As complete genome sequence information became available for many organisms, a novel family of proteins containing a tandem pentapeptide repeat was discovered that can be approximately described by…

Structural Biology of Mammalian Chromatin High Mobility Group Protein HMGA1 and UV-Damaged DNA

Lead Institution

Washington State University

Principal Investigator

Michael Smerdon

Project type

Capability Research

Chromatin high mobility group protein I (HMGA1) belongs to a family of nonhistone mammalian proteins that are thought to be important structural components affecting the conformation and function of…

Enamel biomineralization: Solution- and solid-state NMR studies of a natural protein-mineral interface

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The protein amelogenin is the central protein that controls the formation of dental enamel, the hardest tissue in the human body. The protein orchestrates the nucleation, growth, and organization of…

A multi-pronged approach to characterizing amelogenin’s role in enamel formation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Biomineralization is the process used by living organism to produce minerals with properties not always found in their equivalent inorganically produced counterparts. In vertebrates, mineralized…

Source Attribution of Biological Weapons (Ricin and Associated Metabolites) Using NMR Spectroscopy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Capability Research

This project will study variations in the chemical and isotopic composition of biological weapons with the goal of developing a robust forensics method for deducing the origins and relatedness of…

Seattle Structural Genomics Center for Infectious Diseases - April 1, 2009 - Oct.1 2009.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Exploratory Research

The Seattle Structural Genomics Center for Infectious Disease (SSGCID) is a consortium of four institutions (Seattle Biomedical Research Institue (SBRI), deCODE biostructures, University of…

SSGCID - Year VII

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Protecting the nation through innovative scientific discovery is the mission of EMSL and the mission of The Seattle Structural Genomics Center for Infectious Disease (SSGCID). The SSGCID is funded by…

COVID-19: High-throughput SARS-CoV-2-Human protein-protein interactome and drug discovery on a nanowell array chip

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ying Zhu

The project aims to develop a high throughput and multiplex affinity purification mass spectrometry (AP-MS) assay by seamless integration of protein microarray and nanoscale proteomics. We will…

Structural Studies of Escherichia coli formamidopyrimidine DNA N-glycosylase and its main biological substrate 8-oxoguanine

Lead Institution

University of Vermont

Principal Investigator

Susan Wallace

Project type

Capability Research

DNA repair plays a fundamental role in human health. Our genomic DNA is under constant assault by endogenous oxidative stress and by exogenous factors associated with our environment. Basic…

Structural Characterization and Interactions of Conformationally Heterogeneous States of Biomolecules

Lead Institution

Universidade Federal de Pernambuco

Principal Investigator

Thereza Soares Da Silva

Project type

Large-Scale EMSL Research

This proposal seeks computational resources for the application of long-scale and enhanced sampling techniques to characterize the inherently flexible and conformationally highly heterogeneous…

Amelogenin: Solution- and solid-state studies of a natural biomineralization process

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Wendy Shaw

The protein amelogenin is the central protein controlling the formation of dental enamel, the hardest tissue in the human body. The protein orchestrates the nucleation, growth, and organization of…

COVID-19: Seattle Structural Genomics Center for Infectious Diseases – Year XIV

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The rapid spread of SARS-CoV-2 through-out the world and its life-changing impact on the day-to-day lives of Americans punctuate the need to be ever cautious of the emergence of new epidemic…

Structure-function correlations of proteins in enamel formation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Wendy Shaw

Project type

Large-Scale EMSL Research

Heterogeneous nucleation is important in the development of biominerals such as bone, teeth and mollusk shells, but often, initial nucleation events are not understood at a mechanistic level. It is…

A Systems Biology Approach to Infectious Diseases Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Project type

Exploratory Research

We describe an integrated program for coupling advanced capabilities in high-throughput transcriptomics, proteomics, and metabolomics with a comprehensive informatics infrastructure and a…

Seattle Structural Genomics Center for Infectious Diseases – Year VIII

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The Seattle Structural Genomics Center for Infectious Disease (http://ssgcid.org), is one of two Structural Genomics Centers (SGCs) funded by NIAID to solve protein structures from biodefense…

A multi-disciplined approach to characterize the functional role of the biomineralization protein amelogenin in enamel formation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Biomineralization is the process used by living organism to produce minerals with properties not always found in their equivalent inorganically produced counterparts. In vertebrates, mineralized…

Structural Proteomics of Myobacterium Tuberculosis

Lead Institution

Los Alamos National Laboratory

Principal Investigator

Thomas Terwilliger

Project type

Capability Research

Project Description: Due to the availability of the complete genome of M. tuberculosis and also in part to our development of a genetic system for studying gene function in M. tuberculosis, our…

The Quaternary Structures of Enamel Proteins at Interfaces Studied by Helium-ion Microscopy (HIM)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Barbara Tarasevich

Project type

Large-Scale EMSL Research

The interactions of proteins at surfaces are of great importance to biomineralizaton processes and to the development and function of biomaterials. Amelogenin is a unique biomineralization protein…

SSGCID Year IX

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

As witnessed by the recent outbreak of Ebola in Africa, developing new drugs to combat emerging and re-emerging infectious diseases is of upmost importance. The National Institute of Allergy and…

Understanding proton movement in [FeFe]-hydrogenase

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joseph Laureanti

This proposed work ultimately aims to understand enzymatic design principles using a highly studied and well-known system by creating a series of mutant metalloenzymes to probe the validity and…

Structural Biology of the Nudix Proteins from the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Stephen Holbrook

Project type

Capability Research

The bacterium Deinococcus radiodurans is able to withstand high doses of ionizing and UV radiation that are lethal to virtually all other organisms (Battista, 1997). It has been suggested that this…

Interaction of Escherichia coli Formamidopyrimidine-DNA Glycosylase (Fpg) with Damaged DNA Containing an 7,8-Dihydro-8-oxoguanine Lesion.

Lead Institution

University of Vermont

Principal Investigator

Susan Wallace

Project type

Capability Research

7,8-Dihydro-8-oxoguanine (8OG) is a stable product of oxidative DNA damage, and is a substrate for base excision repair (BER) by DNA glycosylases that recognize and remove the damaged base. In…

Enamel biomineralization: A multi-disciplined approach to understand a natural protein-mineral interface.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

The protein amelogenin is the central protein controlling the formation of dental enamel, the hardest tissue in the human body. The protein orchestrates the nucleation, growth, and organization of…

The role of structural and solvation dynamics of matrix proteins on apatite mineralization

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jinhui Tao

Project type

Exploratory Research

Our goal is to identify the spatial distributions, solvent and structural dynamics of matrix proteins (such as amelogenin, collagen) with joint ultrahigh spatial resolution as well as structural…

Structural Proteomics of Myobacterium Tuberculosis

Lead Institution

Los Alamos National Laboratory

Principal Investigator

Thomas Terwilliger

Project type

Capability Research

Project Description: Due to the availability of the complete genome of M. tuberculosis and also in part to our development of a genetic system for studying gene function in M. tuberculosis, our…

Projects