Gerard Harbison

University of Nebraska - Lincoln

Projects

Investigation of the role of Mg2+ in DNA repair proteins APE1, Pol ?, and FEN1

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Paul Ellis

Project type

Capability Research

We propose to utilize unique capabilities within the EMSL (low temperature solid-state NMR and the mpp2 super computer) to investigate Mg2+ binding to two DNA repair proteins Pol ? and APE1. In the…

High-field spikelet NMR of 65Cu in reduced azurin and (Cu, Zn) superoxide dismutase

Lead Institution

University of Nebraska - Lincoln

Principal Investigator

Gerard Harbison

Project type

Capability Research

High-field 65Cu studies are proposed on the reduced, diamagnetic Cu(I) forms of two important metalloproteins, the blue copper protein azurin, and the essential erythrocyte enzyme (Cu, Zn) superoxide…

Correlating the active site structure of copper metalloproteins with their spectroscopic and chemical properties

Lead Institution

University of Nebraska - Lincoln

Principal Investigator

Gerard Harbison

Project type

Large-Scale EMSL Research

We propose to implement 63Cu and 65Cu, in combination with quantum chemical calculations, to study the metal sites of blue copper proteins, oxidase enzymes, and copper chaperones. Living systems &…