Himadri Pakrasi

Membrane Biology Research Campaign: Use of synthetic biology to probe molecular machines in photosynthesis

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

Project type

Research Campaign

We have shown in our previous EMSL collaborations our ability to develop systems-level models of cyanobacterial processes. In the present proposal, our objective is to characterize and…

Rhizosphere Research Campaign: The plant-soil interface - understanding dynamic interactions in the context of environmental change

Lead Institution

University of Missouri - Columbia

Principal Investigator

Gary Stacey

Project type

Research Campaign

The ultimate, long-term goal is to determine what drives source-sink relationships that control the cycling of plant nutrients belowground. To fully appreciate the complexities of this dynamic…

Investigation of protein degradation mechanisms in Synechococcus elongates UTEX 2973

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

The recently identified and characterized unicellular cyanobacteria, Synechococcus elongates UTEX 2973, a fast growing and genetically malleable organism, has the potential to become the definitive…

Structure of Holo-PSII from Synechocystis sp. PCC 6803

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

Photosystem II (PSII) is a multi-subunit thylakoid membrane protein that catalyzes a unique reaction in nature. The most highly active form of this enzyme is a dimeric form that has multiple membrane…

Cryo-EM analysis of the ultrastructure of Anabaena 33047, a filamentous nitrogen-fixing cyanobacterium

Lead Institution

Washington University in St. Louis

Principal Investigator

Michelle Liberton

Anabaena sp. ATCC 33047 is a fast-growing diazotrophic cyanobacterium and potential bioproduction platform. To protect the O2-sensitive nitrogenase enzyme, Anabaena 33047 filaments differentiate…

Proteomic analysis of the cyanobacterium Synechocystis sp. PCC 6803

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

Project type

Exploratory Research

Cyanobacteria are oxygenic photo-autotrophic prokaryotes that are responsible for roughly 50% of the primary productivity in oceans and lakes. Among the oxygenic photosynthetic organisms…

Grand Challenge in Membrane Biology

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

Project type

Grand Challenge

In this grand challenge, we will use a systems approach to understand the network of genes and proteins that govern the structure and function of membranes and their components responsible for…

Structural Studies of a Novel Family of Manganese Uptake Proteins in Cyanothece Containing a Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

Manganese is an essential trace element that all organisms require. Often, this metal is found in the enzyme?s active site, such as in ribonucleotide reductase, manganese-catalase, and manganese…

Structural Studies of a Family of Proteins from the Diurnal Cyanobacteria Cyanothece 51142 that Contain an Unusual Repeated Five-Residues Domain (RFR).

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Garry Buchko

Project type

Grand Challenge

As complete genome sequence information became available for many organisms, a novel family of proteins containing a tandem pentapeptide repeat was discovered that can be approximately described by…

Dynamic Changes in Molecular Interactions along the Circadian Rhythm (Orr-LDRD Membrane Biology GC, PNNL Scope #90001)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Galya Orr

Project type

Grand Challenge

One of the most challenging tasks in cell biology is the study of the molecular interactions in the natural cellular environment in order to gain information about their dynamic changes in time and…

Cyanobacteria Membrane Biology Grand Challenge: Systems Analysis of the Dynamics of Membrane Architecture, Composition, and Function- Proteomic, Metabolomic, and Metallomic Characterization

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Project type

Grand Challenge

The strain Cyanothece sp. ATCC 51142, isolated from benthic waters off the coast near Galveston, Texas, is a unicellular strain approximately 4-7 &#956;m in diameter along the major axis. This…

Abundance of protein components in photosystem II protein complex purified from mutant cyanobacterial cells lacking individual subunit protein

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

Project type

Exploratory Research

Photosystem II (PSII) is the pigment-protein complex responsible for the process of converting water to molecular oxygen and subsequent carbon fixation during oxygenic photosynthesis. PSII consists…

Development of a systems-level model of cellular dynamics in response to different environmental conditions in cyanobacteria

Lead Institution

Purdue University

Principal Investigator

Louis Sherman

Project type

Large-Scale EMSL Research

This proposal will utilize a systems biology approach to develop a knowledgebase of the metabolic and regulatory networks that can ultimately be used for the photobiological production of advanced…

Determination of the protein composition of a novel membrane bound pigment protein complex involved in solar energy capture

Lead Institution

Washington University in St. Louis

Principal Investigator

Himadri Pakrasi

Project type

Limited Scope

Photosynthetic organisms such as cyanobacteria can efficiently convert sunlight into usable forms of energy. These organisms use pigment protein antenna systems to capture light and transfer it to…

EMSL Science Overview

Himadri Pakrasi

Projects

Membrane Biology Research Campaign: Use of synthetic biology to probe molecular machines in photosynthesis

Rhizosphere Research Campaign: The plant-soil interface - understanding dynamic interactions in the context of environmental change

Investigation of protein degradation mechanisms in Synechococcus elongates UTEX 2973

Structure of Holo-PSII from Synechocystis sp. PCC 6803

Cryo-EM analysis of the ultrastructure of Anabaena 33047, a filamentous nitrogen-fixing cyanobacterium

Proteomic analysis of the cyanobacterium Synechocystis sp. PCC 6803

Grand Challenge in Membrane Biology

Structural Studies of a Novel Family of Manganese Uptake Proteins in Cyanothece Containing a Repeated Five-Residues Domain (RFR).

Structural Studies of a Family of Proteins from the Diurnal Cyanobacteria Cyanothece 51142 that Contain an Unusual Repeated Five-Residues Domain (RFR).

Dynamic Changes in Molecular Interactions along the Circadian Rhythm (Orr-LDRD Membrane Biology GC, PNNL Scope #90001)

Cyanobacteria Membrane Biology Grand Challenge: Systems Analysis of the Dynamics of Membrane Architecture, Composition, and Function- Proteomic, Metabolomic, and Metallomic Characterization

Abundance of protein components in photosystem II protein complex purified from mutant cyanobacterial cells lacking individual subunit protein

Development of a systems-level model of cellular dynamics in response to different environmental conditions in cyanobacteria

Determination of the protein composition of a novel membrane bound pigment protein complex involved in solar energy capture