Jared Shaw

Synthesis and Structural Probing of Protein Networks in Vitamin B6 Biosynthesis.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Irina El Khoury

We propose to develop a multi-modal experimental pipeline, integrating multiple existing and emerging EMSL capabilities, including cell-free protein expression, Native Mass Spectrometry (Native MS),…

Development of an Integrated Structural Mass Spectrometry Platform for Unveiling the Structure of an Unknown Protein Complex Machinery in Lignin Synthesis

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Multiple distinct Lignin-Forming Complexes (LFC) are proposed to be templates and catalysts that orchestrate lignin synthesis in plants. It is hypothesized that each LFC consists of one or more…

Advanced MS instrumentation and methods for functional proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jared Shaw

This proposal addresses a critical need to continue to upgrade the spectrometer for the 21T FTICR-MS in order to keep this flagship capability on the leading edge of technology. The proposed work…

Native Mass Spectrometry Enabled Electron Microscopy for Visual Proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jared Shaw

Develop a soft-landing capability for native protein complexes coupled with electron microscopy (EM) to enable visual proteomics. Sample heterogeneity greatly limits the resolution of 3D structures…

Development and Implementation of Online Separation Techniques Coupled to Native Mass Spectrometry for Characterization of Endogenous Proteins Extracted from Biological Matrices

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Recent developments of state-of-the-art mass spectrometry (MS) instrumentation at EMSL, including the 21T Fourier Transform Ion Cyclotron Resonance (FTICR) MS, have enabled high resolution and high…

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Development of in vitro glycosylation toolkit to study eukaryotic glycoproteins

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Irina El Khoury

When working with glycoproteins from native sources, biochemical analysis is often hampered by sample heterogeneity and glycan-postprocessing structural diversity. It is therefore desirable to create…

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Position-specific stable isotope analysis for elucidating provenance and fate of organic matter

Lead Institution

Michigan State University

Principal Investigator

James Moran

The EMSL 21T FTICR MS is amongst the highest mass resolution of any mass spec ever developed (> 6M at m/z 700 (Shaw et al., 2016)). We seek to provide a proof of concept that suitable stability…

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Advanced 21T FTICR MS instrumentation and methods for functional proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

William Kew

The 21T FTICR-MS at the EMSL provides extremely high mass accuracy and resolution enabling superior depth of characterization for natural organic matter and intact mass measurement of denatured…

Atmospheric Soil Organic Particles

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Recent discovery of atmospheric soil organic particles (ASOP), emitted through previously unrecognized atmosphere-land-surface interactions, suggests that ASOP may have a widespread impact over a…

Probing complex microbiomes using mass spectrometry and sequencing capabilities to understand how microbiomes are influenced by their environment

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Stephen Callister

This proposal will utilize next generation sequencing and mass spectrometry (MS) capabilities to address key knowledge gaps in our functional understanding of how complex microbiomes influence and…

Proteomic Insights into Mechanisms of Microbial Dormancy

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jared Shaw

Ecologically important processes such as carbon and nutrient cycling largely depend on the active fraction of microbial communities, and only a small portion of the total soil microbiome is active…

Integrated Stable Isotope Probing for the Comprehensive Quantitative- Characterization of Biological Dynamics and Elemental Cycling

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mary Lipton

Biological organisms dynamically function in relationship to their environment and neighboring organisms, creating a continual interplay that constitutes the ecological state. Fundamental…

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Probing complex microbiomes using mass spectrometry and sequencing capabilities to understand how microbiomes are influenced by their environment

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Stephen Callister

This is a resource owner proposal as part of the MinT LDRD Initiative. The objective of this proposal is the development of sequencing and mass spectrometry capabilities to support research into how…

Atmospheric Soil Organic Particles

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Recent discovery of atmospheric soil organic particles (ASOP), emitted through previously unrecognized atmosphere-land-surface interactions, suggests that ASOP may have a widespread impact over a…

Development of an ECD cell for biomolecular characterization

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jared Shaw

The ultimate goal of this proposal is to integrate an electron capture dissociation (ECD) cell into ThermoFisher Scientific’s Exactive series mass spectrometers. The proposed work in collaboration…

EMSL Science Overview

Jared Shaw

Projects

Synthesis and Structural Probing of Protein Networks in Vitamin B6 Biosynthesis.

Development of an Integrated Structural Mass Spectrometry Platform for Unveiling the Structure of an Unknown Protein Complex Machinery in Lignin Synthesis

Advanced MS instrumentation and methods for functional proteomics

Native Mass Spectrometry Enabled Electron Microscopy for Visual Proteomics

Development and Implementation of Online Separation Techniques Coupled to Native Mass Spectrometry for Characterization of Endogenous Proteins Extracted from Biological Matrices

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Development of in vitro glycosylation toolkit to study eukaryotic glycoproteins

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Position-specific stable isotope analysis for elucidating provenance and fate of organic matter

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Advanced 21T FTICR MS instrumentation and methods for functional proteomics

Atmospheric Soil Organic Particles

Probing complex microbiomes using mass spectrometry and sequencing capabilities to understand how microbiomes are influenced by their environment

Proteomic Insights into Mechanisms of Microbial Dormancy

Integrated Stable Isotope Probing for the Comprehensive Quantitative- Characterization of Biological Dynamics and Elemental Cycling

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Probing complex microbiomes using mass spectrometry and sequencing capabilities to understand how microbiomes are influenced by their environment

Atmospheric Soil Organic Particles

Development of an ECD cell for biomolecular characterization