Skip to main content

John Melchior

Institution
Pacific Northwest National Laboratory

Projects

Targeted Nanobody Generation

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Samantha Powell
Project type
Contracted Time
Here we introduce a nanobody screening pipeline for the generation of target specific nanobodies. Nanobodies will be screened against a synthetic yeast library and then undergo alternating rounds of…

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Cort
PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

Towards proteome-wide assessment of cellular landscapes

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
We will develop a novel workflow to characterize proteome-wide structural changes of cellular landscapes between different cell states (or cell types). The goal is to bridge the gap between cellular…

Impact of apolipoprotein E isoforms on brain lipoproteins

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Melchior
Cerebrovascular dysfunction leads to the deposition of amyloid plaques and neurofibrillary tangles in the brain which underlie the development of Alzheimer’s disease (AD); an epidemic that…

A Molecular Atlas of the Developing Lung

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Joshua Adkins
The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our understanding of alveologenesis is a critical step…

Structural profiling of the molecular interactome to define phenotype

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
John Melchior
The functional pathways that define phenotype are reflected by protein quinary structure; the transient encounters of proteins with other proteins, molecules, cofactors, and nucleic acids that…

A Molecular Atlas of the Developing Lung

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Joshua Adkins
The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our understanding of alveologenesis is a critical step…