John Melchior

The molecular basis for the role of apolipoprotein A-II in cholesterol and triglyceride metabolism

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Project type

Cryo-EM Only

Apolipoprotein (apo)A-II is an abundant human plasma protein primarily in high-density lipoproteins (HDL) but also in very low density lipoproteins (VLDL) and chylomicrons. Despite a large literature…

Enhancing biopreparedness through a model system to understand the molecular mechanisms that lead to pathogenesis and disease transmission

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

The science of biopreparedness to counter biological threats hinges on understanding the fundamental principles and molecular mechanisms that lead to pathogenesis and disease transmission. Our vision…

Targeted Nanobody Generation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Samantha Powell

Project type

Contracted Time

Here we introduce a nanobody screening pipeline for the generation of target specific nanobodies. Nanobodies will be screened against a synthetic yeast library and then undergo alternating rounds of…

Identification of HLA-A29 peptides bound to the MHC-Class I groove in birdshot uveitis patient leukocytes.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Birdshot chorioretinopathy (BSCR) is an autoimmune disorder that causes blindness. All patients with BSCR have the HLA-A29 allele encoded by the HLA-A29 MHC Class I receptor. However, less than 1%…

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

Predicting adverse biological effects for chemicals of concern through integration of functional profiling with artificial intelligence/machine learning (AI/ML)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We will use thermal proteome profiling (TPP) to identify protein targets and pathways affected by exposure to a chemical of concern (COC). As a next step, we will use an artificial intelligence…

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

PPS is a semisynthetic heterogeneous sulfated polysaccharide derived from xylan, the β-1,4-linked polymer of xylose that is the backbone structural component of hemicellulose in most types of wood…

Manipulation of Host Signature Expression Following Respiratory Virus Infection

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Pathogen invasion of host cells causes a myriad of functional changes including alterations of chromatin accessibility often limiting defense responses, shunting of cellular resources to centers of…

A Molecular Atlas of the Developing Lung

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our understanding of alveologenesis is a critical step…

Integrated multi-modal approach for structure and function determination of lignin-forming protein complexes isolated from plants

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

We propose to apply an integrated multi-modal structural biology approach to investigate a biosynthetic protein complex in plants that is hypothesized to organize and guide lignin biosynthesis via…

Novel Interactomics Workflow for Protein-Protein and Protein-Lipid Interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Stephanie Thibert

Macromolecular assemblies in cell membrane and organelles are compositionally and structurally complex and their identification and characterization represent a major barrier to understanding…

Towards proteome-wide assessment of cellular landscapes

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

We will develop a novel workflow to characterize proteome-wide structural changes of cellular landscapes between different cell states (or cell types). The goal is to bridge the gap between cellular…

Impact of apolipoprotein E isoforms on brain lipoproteins

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Cerebrovascular dysfunction leads to the deposition of amyloid plaques and neurofibrillary tangles in the brain which underlie the development of Alzheimer’s disease (AD); an epidemic that…

The molecular basis for the role of apolipoprotein A-II in cholesterol and triglyceride metabolism

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Apolipoprotein (apo)A-II is an abundant human plasma protein primarily in high-density lipoproteins (HDL) but also in very low density lipoproteins (VLDL) and chylomicrons. Despite a large literature…

A Molecular Atlas of the Developing Lung

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our understanding of alveologenesis is a critical step…

Structural profiling of the molecular interactome to define phenotype

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

The functional pathways that define phenotype are reflected by protein quinary structure; the transient encounters of proteins with other proteins, molecules, cofactors, and nucleic acids that…

Detergent-free extraction of membrane proteins and lipids from native cells

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

Membrane proteins account for >30% of most genomes, yet only make up <4% of reported high-resolution structures in the protein data bank. Current approaches for isolating membrane proteins from…

EMSL Science Overview

John Melchior

Projects

The molecular basis for the role of apolipoprotein A-II in cholesterol and triglyceride metabolism

Enhancing biopreparedness through a model system to understand the molecular mechanisms that lead to pathogenesis and disease transmission

Targeted Nanobody Generation

Identification of HLA-A29 peptides bound to the MHC-Class I groove in birdshot uveitis patient leukocytes.

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Predicting adverse biological effects for chemicals of concern through integration of functional profiling with artificial intelligence/machine learning (AI/ML)

Pentosan Polysulfate Sodium Profiling with Mass Spectrometry

Manipulation of Host Signature Expression Following Respiratory Virus Infection

A Molecular Atlas of the Developing Lung

Integrated multi-modal approach for structure and function determination of lignin-forming protein complexes isolated from plants

Novel Interactomics Workflow for Protein-Protein and Protein-Lipid Interactions

Towards proteome-wide assessment of cellular landscapes

Impact of apolipoprotein E isoforms on brain lipoproteins

The molecular basis for the role of apolipoprotein A-II in cholesterol and triglyceride metabolism

A Molecular Atlas of the Developing Lung

Structural profiling of the molecular interactome to define phenotype

Detergent-free extraction of membrane proteins and lipids from native cells