Liang Shi | Environmental Molecular Sciences Laboratory

Investigating the Effects of Geologic CO2 Sequestration on Microbial Physiology, Metabolism and Functionality.

Lead Institution

Colorado State University

Principal Investigator

Michael Wilkins

Project type

Large-Scale EMSL Research

The injection of greenhouse gases into deep geologic formations offers a promising strategy for reducing emissions into the atmosphere that contribute to global warming. The injection of CO2 as a…

Investigating the Effects of Geologic CO2 Sequestration on Microbial Physiology, Metabolism and Functionality.

Lead Institution

Colorado State University

Principal Investigator

Michael Wilkins

Project type

Exploratory Research

The injection of greenhouse gases into deep geologic formations offers a promising strategy for reducing emissions into the atmosphere that contribute to global warming. The injection of CO2 as a…

The Role of Microenvironments and Physicochemical Subsurface Components on Field Scale Biogeochemistry and Contaminant Mobility

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Exploratory Research

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Unraveling the Molecular Biology of Host-Pathogen Interactions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Liang Shi

Project type

Exploratory Research

Macrophages play an important role in the pathogenesis of Salmonella infection. Critical to the ability of macrophages to kill the Salmonella is the activity of the divalent metal ion transporter…

Role of Microenvironments and Transition Zones in Subsurface Reactive Contaminant Transport

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Protein Complex Identification using novel affinity resins

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

M. Mayer-Cumblidge

Project type

Exploratory Research

We are validating the use of a FlAsH resin with a dithiol releasing agent for high-throughput protein complex identification We are going to do endogenous pull-downs of protein complexes from S…

Role of Microenvironments and Transition Zones in Subsurface Reactive Contaminant Transport

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Protein Complex Identification using novel affinity resins

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

M. Mayer-Cumblidge

We are validating the use of a FlAsH resin with a dithiol releasing agent for high-throughput protein complex identification We are going to do endogenous pull-downs of protein complexes from S…

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL.PNNL Scientific Focus Area. The PNNL SFA will develop a predictive understanding…

Thrust Area 1: Characterization of Purified Proteins for Distribution to Grand Challenge Participants (EMSL User Support)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Liang Shi

Project type

Grand Challenge

A facultative anaerobic bacterium, Shewanella oneidensis MR-1 is able to use metal oxides, such as Mn(IV) and Fe(III), as terminal electron acceptors during anaerobic respiration. Since Mn(IV) and…

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL.PNNL Scientific Focus Area. The PNNL SFA will develop a predictive understanding…

Thrust 4: Model Hematite Thin Films and Bacterial Iron Reduction (LDRD: Cytochrome and Whole Cell Interactions with Iron Oxides)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Timothy Droubay

Project type

Grand Challenge

This LDRD project is the fourth one associated with the Biogeochemistry Grand Challenge (BGC). Its overall goal is to develop new capabilities and materials as needed to understand the molecular…

Genomes-to-Life Protein Complex Isolation Capability

Lead Institution

Western University of Health Sciences

Principal Investigator

Thomas Squier

Project type

Exploratory Research

We have established a pilot proteomics facility in support of the "Goal 1" of the Department of Energy's Genomes to Life program, which seeks to identify 80% of the protein complexes…

Protein Adsorption at the Metal Oxide/Water Interface by Second Harmonic Generation

Lead Institution

University of Wyoming

Principal Investigator

Carrick Eggleston

Project type

Large-Scale EMSL Research

This EMSL Partner Proposal is designed to push EMSL’s current capability in Second Harmonic Generation spectroscopy to analyze protein adsorption at the mineral/water interface both on single…

Development of Cell Permeable Dyes for in-vivo Live Cell Imaging

Lead Institution

Western University of Health Sciences

Principal Investigator

Thomas Squier

Project type

Exploratory Research

. Our long-term goal is to develop the necessary reagents and technology for the rapid identification of a substantial fraction of the multiprotein complexes in an organism, thereby permitting a…

Specificity of Shewanella oneidensis outer membrane cytochromes
OmcA and OmcB towards iron- and manganese-oxide surfaces

Lead Institution

The Ohio State University

Principal Investigator

Brian Lower

Project type

Large-Scale EMSL Research

Metal-reducing bacteria have a unique ability to reduce solid-phase iron- and manganese- oxides in anaerobic environments. Unlike oxygen, Fe and Mn oxides, in a solid form, cannot diffuse across the…

Electrochemical analyses of the electron transfer rates of MtrC and OmcA to hematite

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Liang Shi

Project type

Large-Scale EMSL Research

Decaheme c-type cytochromes MtrC and OmcA of Shewanella oneidensis MR-1 are hypothesized as terminal reductases to the insoluble forms of iron and manganese oxides [Fe(III)/Mn(III/IV)] because they…

Role of Microenvironments and Transition Zones in Subsurface Reactive Contaminant Transport

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Exploratory Research

The PNNL Scientific Focus Area (SFA) will resolve critical Hanford and basic subsurface science issues through integrated, multi-disciplinary, science-theme focused research on the role of…

Regulating Space: Bacterial Mastery of the Host Actin Cytoskeleton

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

Project type

Large-Scale EMSL Research

Abstract: The host plasma membrane, the landscape for environmental and physical perturbations by many microbial pathogens, enhances or diminishes signal transduction by altering diffusivity and…

Role of Microenvironments and Transition Zones in Field scale microbial ecology and biogeochemistry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Exploratory Research

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Role of Microenvironments and Transition Zones in Field Scale Microbial Ecology and Biogeochemistry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Limited Scope

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Characterization of Effector and Host Proteins Involved in Host-Pathogen Interactions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Project type

Exploratory Research

The multiple mechanisms by which pathogenic enterobacteria of the genus Salmonella infect their host indicate a complex host-pathogen relationship. The pathogen secretes dozens of proteins, termed &…

Projects