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Ljiljana Pasa-Tolic

Institution
Environmental Molecular Sciences Laboratory

Projects

Genome-Enabled Characterization of Orphan Receptor-Like Kinases in Plants

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
This research will characterize the functions and clients (i.e., substrates) of receptor-like kinases (RLKs) of Arabidopsis, rice, and soybean. Objective 1 will reveal RLK functions by combing…

Spatial Metabolomics and Glycomics for Human Kidneys

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christopher Anderton
Acute kidney injury (AKI) and chronic kidney disease (CKD) are major contributors to overall morbidity and mortality in patients in the US. However, we lack a clear understanding of the major…

Developing automatic gain control for organic matter analyses by FTICR-MS

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Kevin Zemaitis
Three commercial FTICR-MS instruments (Bruker; 7T, 12T, and 15T) routinely support organic matter (OM) analyses in EMSL. Generally speaking, the quality of data produced is inherently linked to the…

Massive single-cell proteomics for cancer biology

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
Single-cell technologies have become the cornerstone of biomedical and cell biology research. Next- generation sequencing-based technologies have enabled large-scale characterization of transcript…

High throughput single cell multiomics platform

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
In this collaborative project, PNNL and Scienion will co-develop an integrated microfluidic technology to co-measure the transcriptome and proteome in single cells. The technology will enable us to…

Proteomic study of Transthyretin Protein

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
The project will establish a scientific partnership between the cardiomyopathy section at OHSU and mass spectrometry facility at PNNL which leverages the strengths of OHSU Cardiomyopathy center …

Collaborative Research Projects Supported by the NCRR P41 Resource Center

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
David Camp
Project type
Large-Scale EMSL Research
The Proteomics Resource Center for Integrative Biology at PNNL was established in 2003 and builds on the unique instrumentation and the infrastructure provided by PNNLs EMSL facility. The mission of…

Mass Spectrometry Imaging of Microbial Communities

Lead Institution
University of California, San Diego
Principal Investigator
Pieter Dorrestein
Project type
Large-Scale EMSL Research
Despite our significant technological advancements, the solutions to many of humanities lingering problems such as bioremediation of toxic heavy metal environmental contaminants, creation of…

Quantifying the functional and structural states of dynamic microbial communities

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
James Fredrickson
Project type
Large-Scale EMSL Research
The overall goal of this proposal is to provide a quantitative framework in which to compare species-resolved patterns of gene expression, allowing comparison of the effects of perturbations of…

Metabolic regulation of O-GlcNAc and cardiac function during hypertrophy

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
Despite treatment advances, half of patients suffering from heart failure die within five years of diagnosis, while those who survive may live with severe disabilities. Cardiac hypertrophy poses a…

Subunit structures of Arabidopsis RNA Polymerase IV complexes

Lead Institution
Indiana University - Bloomington
Principal Investigator
Craig Pikaard
Project type
Large-Scale EMSL Research
All eukaryotes have three conventional nuclear DNA-dependent RNA polymerases, abbreviated as Pol I, Pol II and Pol III. Pol I transcribe 45S pre-rRNA, pol II transcribes mRNAs and pol III…

3D Root Cartography of Molecular-Microbe Interactions

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christer Jansson
We intend to develop a pipeline for simultaneous 3D spatial imaging of metabolites and microorganisms on plant roots, generating a molecular and microbial 3D-cartography of the root system. By…

Microbes persist: Systems biology of the soil microbiome

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
Microorganisms play key roles in soil carbon turnover and stabilization of persistent organic matter via their metabolic activities, cellular biochemistry, and extracellular products. Microbial…

Protein modifications associated with hibernation.

Lead Institution
University of Colorado
Principal Investigator
Lois Epperson
Project type
Limited Scope
Liver samples from the hibernator, golden-mantled ground squirrel, when separated by 2D gel electrophoresis, demonstrate a quantifiable and as yet undefined physical modification that manifests…

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
David Koppenaal
Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Consortium for Advanced Sorghum Phenomics (CASP)

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christer Jansson
The mission of the proposed project is to accelerate breeding of biomass sorghum to maximize terminal compositional (e.g., lignocellulosic) yield under drought and saline field conditions using novel…

Soybean Proteomics

Lead Institution
University of Missouri - Columbia
Principal Investigator
Gary Stacey
Project type
Large-Scale EMSL Research
Soybean is the major source of vegetable oil and, therefore, a major source for biodiesel production. In recognition of this fact, the Department of Energy-Joint Genome Institute recently completed a…

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
David Koppenaal
Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

EPICON: Epigenetic Control of Drought Acclimation in Sorghum

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christer Jansson
Genetic manipulation of crops to increase the presence or intensity of desirable traits has been critical to increasing agricultural productivity. These changes have primarily involved modification…

Development of ETD-enhanced FT-ICR MS

Lead Institution
Bruker Daltonics Inc.
Principal Investigator
Gary Kruppa
Project type
Scientific Partner
The objective of this proposed one-year EMSL-Bruker Daltonics partnership is to incorporate electron transfer dissociation (ETD)-- relatively new dissociation technique--with EMSL's 12-Tesla…

Metabolic regulation of O-GlcNAc and cardiac function during hypertrophy

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
Despite treatment advances, half of patients suffering from heart failure die within five years of diagnosis, while those who survive may live with severe disabilities. Cardiac hypertrophy poses a…

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
David Koppenaal
Fourier transform ion cyclotron mass spectrometry (FTICR-MS), the highest performance mass spectrometry technique, is uniquely valued as a research tool for analytical applications requiring the…