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Ljiljana Pasa-Tolic

Institution
Environmental Molecular Sciences Laboratory
ORCID
ORCID logo 0000-0001-9853-5457

Projects

Genome-Enabled Characterization of Orphan Receptor-Like Kinases in Plants

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
This research will characterize the functions and clients (i.e., substrates) of receptor-like kinases (RLKs) of Arabidopsis, rice, and soybean. Objective 1 will reveal RLK functions by combing…

Spatial Metabolomics and Glycomics for Human Kidneys

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christopher Anderton
Acute kidney injury (AKI) and chronic kidney disease (CKD) are major contributors to overall morbidity and mortality in patients in the US. However, we lack a clear understanding of the major…

Developing automatic gain control for organic matter analyses by FTICR-MS

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Kevin Zemaitis
Three commercial FTICR-MS instruments (Bruker; 7T, 12T, and 15T) routinely support organic matter (OM) analyses in EMSL. Generally speaking, the quality of data produced is inherently linked to the…

Massive single-cell proteomics for cancer biology

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
Single-cell technologies have become the cornerstone of biomedical and cell biology research. Next- generation sequencing-based technologies have enabled large-scale characterization of transcript…

High throughput single cell multiomics platform

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
In this collaborative project, PNNL and Scienion will co-develop an integrated microfluidic technology to co-measure the transcriptome and proteome in single cells. The technology will enable us to…

Proteomic study of Transthyretin Protein

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
The project will establish a scientific partnership between the cardiomyopathy section at OHSU and mass spectrometry facility at PNNL which leverages the strengths of OHSU Cardiomyopathy center …

Spatial Metabolomics and Glycomics for Human Kidneys

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christopher Anderton
Acute kidney injury (AKI) and chronic kidney disease (CKD) are major contributors to overall morbidity and mortality in patients in the US. However, we lack a clear understanding of the major…

Development of the Integrated MetaPROteomics Viewer (IMPROV) Software Toolkit

Lead Institution
University of British Columbia
Principal Investigator
Steven Hallam
Project type
Scientific Partner
Beyond the periphery of our senses exists an infinitely diverse microbial world. This microcosm extends into every imaginable habitat, thrives under powerful extremes, and helps create and sustain…

High resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
David Koppenaal
Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
David Koppenaal
Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Deep UV laser ablation system for spatial single-cell proteomics

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
William Chrisler
Laser capture microdissection (LCM) is the key capability for spatially resolved omicss. However, commercially available LCM systems are designed for mammalian tissues and feature UV lasers (330 nm),…

Rhizosphere processes at molecular to core scale

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Nancy Hess
The amount of C in soil far exceeds the C content of atmosphere as well as the amount C stored above ground as plant biomass. The C fluxes between atmosphere and biomass are huge, many orders of…

Consortium for Advanced Sorghum Phenomics (CASP)

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christer Jansson
The mission of the proposed project is to accelerate breeding of biomass sorghum to maximize terminal compositional (e.g., lignocellulosic) yield under drought and saline field conditions using novel…

Towards proteome-wide assessment of cellular landscapes

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
We will develop a novel workflow to characterize proteome-wide structural changes of cellular landscapes between different cell states (or cell types). The goal is to bridge the gap between cellular…

Identification and quantification of biomarkers of malaria infection.

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Kim Hixson
This application is for the continuation of the currently closed proposal 8211 "Membrane proteins of placental parasites". We have a joint grant proposal between Richard D. Smith and Michal…

Soybean Proteomics

Lead Institution
University of Missouri - Columbia
Principal Investigator
Gary Stacey
Project type
Large-Scale EMSL Research
The USA is one of the world's largest producers of soybean (Glycine max), which is important as a source of animal feed, oil and, more recently, for biodiesel production. In recognition of this…

EPICON: Epigenetic Control of Drought Acclimation in Sorghum

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christer Jansson
Genetic manipulation of crops to increase the presence or intensity of desirable traits has been critical to increasing agricultural productivity. These changes have primarily involved modification…

Technology development in support of NCRR

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Nancy Colton
The Pacific Northwest National Laboratory (PNNL) has built a facility that already stands at the forefront of proteomic research. Moreover, the Center is uniquely poised for future developments. We…

EPICON: Epigenetic Control of Drought Acclimation in Sorghum

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christer Jansson
Genetic manipulation of crops to increase the presence or intensity of desirable traits has been critical to increasing agricultural productivity. These changes have primarily involved modification…

Spatial Metabolomics for Human Kidneys

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Christopher Anderton
Acute kidney injury (AKI) and chronic kidney disease (CKD) are major contributors to overall morbidity and mortality in patients in the US. However, we lack a clear understanding of the major…

Mechanistic studies of AAP and capsid assembly of AAV vectors

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
Adeno-associated viruses (AAV) have become increasingly popular as vectors for human gene therapy. However, the requirement for high vector doses needed to achieve therapeutic efficacy poses a…

Collaborative Research Projects Supported by the NCRR P41 Resource Center

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
David Camp
Project type
Large-Scale EMSL Research
The Proteomics Resource Center for Integrative Biology at PNNL was established in 2003 and builds on the unique instrumentation and the infrastructure provided by PNNLs EMSL facility. The mission of…

Mass Spectrometry Imaging of Microbial Communities

Lead Institution
University of California, San Diego
Principal Investigator
Pieter Dorrestein
Project type
Large-Scale EMSL Research
Despite our significant technological advancements, the solutions to many of humanities lingering problems such as bioremediation of toxic heavy metal environmental contaminants, creation of…

Quantifying the functional and structural states of dynamic microbial communities

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
James Fredrickson
Project type
Large-Scale EMSL Research
The overall goal of this proposal is to provide a quantitative framework in which to compare species-resolved patterns of gene expression, allowing comparison of the effects of perturbations of…