Matthew Marshall

Role of Microenvironments and Transition Zones in Field Scale Microbial Ecology and Biogeochemistry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Limited Scope

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Multimodal Nanoscale Chemical Imaging of Intra- and Inter-Cellular Biomolecules Involved in Microbial Interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Lea

Microbes orchestrate a suite of intra- and inter-cellular biomolecules to carry out diverse metabolic processes. Existing genomic and biochemical methods cannot directly probe the physical…

Correlative High-Resolution Imaging and Spectroscopy to Characterize the Structure and Biogeochemical Function of Microbial Biofilms

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Matthew Marshall

Project type

Exploratory Research

This proposal leverages knowledge and experience gained from DOE-funded research to elucidate the mechanisms involved in dissimilatory metal reduction, as well as, microscale imaging and analytical…

The Role of Microenvironments and Physicochemical Subsurface Components on Field Scale Biogeochemistry and Contaminant Mobility

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Exploratory Research

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Multi-System Analysis of Microbial Biofilms

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Matthew Marshall

Project type

Large-Scale EMSL Research

Abstract: This proposed research will employ state-of-the-art technologies at the Environmental Molecular Sciences Laboratory (EMSL) for determining chemical composition in relation to the native…

Role of Microenvironments and Transition Zones in Subsurface Reactive Contaminant Transport

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Role of Microenvironments and Transition Zones in Subsurface Reactive Contaminant Transport

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL.PNNL Scientific Focus Area. The PNNL SFA will develop a predictive understanding…

In situ mesoscale imaging of biofilm dynamics using SALVI

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Xiao-Ying Yu

Understanding how metabolic pathways are interconnected and controlled at the subcellular scale within living systems with coexisting macromolecules in a cell that has complex structure, dynamics,…

Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

FedEx1

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alex Beliaev

Project type

Exploratory Research

Bacterial strains such as Shewanella oneidensis strain MR-1 are key organisms in the bioremediation of metals due to their ability to enzymatically reduce and precipitate a diverse range of heavy…

Impact of phenazine-1-carboxylic acid upon biofilm development in the rhizosphere of dryland and irrigated wheat

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Matthew Marshall

Project type

Limited Scope

Rapid access is necessary for completion of six months work required for an Office of Science Graduate Student Research award by the end of the award period lasting from January 21, 2015 to July 21,…

The in vivo localization and interactions between structural components of the Shewanella oneidensis MR-1 metal reducing system by high resolution transmission electron microscopy.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Matthew Marshall

Project type

Grand Challenge

To understand the mechanisms of anaerobic metal oxide reduction, important issues regarding the subcellular localization and the outer membrane architecture of the iron-reducing respiratory chain…

Impact of phenazine-1-carboxylic acid upon biofilm development, Fe speciation, and the fate of microbial biomass in the rhizosphere of dryland and irrigated wheat

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Matthew Marshall

Project type

Exploratory Research

Rhizobacterial biofilms are a major sink for plant-derived carbon, and a major source for microbially-derived soil organic matter (SOM). Bacteria residing within biofilm matrices, which restrict…

The biogeochemical mechanisms controlling reduced radionuclide particle formation properties and stability.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Matthew Marshall

Project type

Exploratory Research

To further understand the mechanisms of anaerobic radionuclide reduction, important issues regarding the subcellular localization of reduced, insoluble metal oxides in association with the outer…

Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Cryo-EM investigations of interfacial bacterial extracellular polymeric substance (EPS) in vanadium interactions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Matthew Marshall

Project type

Large-Scale EMSL Research

In anaerobic conditions, dissimilatory metal-reducing bacteria such as Shewanella oneidensis MR-1 can reduce a variety of polyvalent metal ions either as soluble complexes or associated with solid…

In situ mesoscale imaging of live biofilm dynamics using SALVI

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

Xiao-Ying Yu

We seek to understand how the dynamics of nutrients, metabolites and metals influence microbial processes in biofilm systems. Our approach will use axenic or heterogeneous microbial communities to…

3-Dimensional Reconstruction and Modeling of Bacterial Extracellular Polymeric Substances (ExPS)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

We propose to apply the method of Cryo-electron tomography for fine scale imaging of the bacterial extracellular polymeric substances (ExPS) in its native state. We will create a 3D model of fully…

Hydro-Biogeochemical Process Dynamics in the Groundwater-Surface Water Interaction Zone

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Zachara

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL.PNNL Scientific Focus Area. The PNNL SFA will develop a predictive understanding…

Role of Microenvironments and Transition Zones in Field scale microbial ecology and biogeochemistry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James McKinley

Project type

Exploratory Research

This is a renewal proposal for a previous collaborative effort between the PNNL Scientific Focus Area (SFA) and EMSL. The research proposed will resolve critical Hanford and basic subsurface science…

Multimodal Nanoscale Chemical Imaging of Intra- and Inter-Cellular Biomolecules Involved in Microbial Interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Lea

Microbes orchestrate a suite of intra- and inter-cellular biomolecules to carry out diverse metabolic processes. Existing genomic and biochemical methods cannot directly probe the physical…

Projects