Nikola Tolic

Systematic Characterization of Protein Glycosylation of Bacteria Cell Surface Proteins

Lead Institution

University of Oklahoma

Principal Investigator

Si Wu

The overall goal of this proposal is to develop an integrated top-down and bottom-up approach for bacterial glycoprotein profiling assisted by accurate glycan structure identification, which will be…

Microbes persist: Systems biology of the soil microbiome

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Microorganisms play key roles in soil carbon turnover and stabilization of persistent organic matter via their metabolic activities, cellular biochemistry, and extracellular products. Microbial…

Imaging and Monitoring the Initial Stages of Biofilm Formation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

R. Shane Addleman

As outlined below, the goal of this study to apply an integrated, multi-faceted approach to the analysis of the initial molecular and cellular processes involved with cell adhesion, colonization, and…

Imaging and Monitoring the Initial Stages of Biofilm Formation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

R. Shane Addleman

As outlined below, the goal of this study to apply an integrated, multi-faceted approach to the analysis of the initial molecular and cellular processes involved with cell adhesion, colonization, and…

Imaging and Monitoring the Initial Stages of Biofilm Formation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

R. Shane Addleman

As outlined below, the goal of this study to apply an integrated, multi-faceted approach to the analysis of the initial molecular and cellular processes involved with cell adhesion, colonization, and…

Rhizosphere Underground:
Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Microorganisms are fundamental to biogeochemical cycling, as microbial metabolism significantly contributes to the regulation of terrestrial carbon and nitrogen cycling. However, the impact of the…

Advancing soil C characterization using HRMS and NMR

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Hess

Soil organic matter consists of diverse and complex mixture of hundreds to thousands biological and metabolic products that are poorly characterized using current techniques. We seek to develop a…

Atmospheric Soil Organic Particles

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Recent discovery of atmospheric soil organic particles (ASOP), emitted through previously unrecognized atmosphere-land-surface interactions, suggests that ASOP may have a widespread impact over a…

Atmospheric Soil Organic Particles

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Recent discovery of atmospheric soil organic particles (ASOP), emitted through previously unrecognized atmosphere-land-surface interactions, suggests that ASOP may have a widespread impact over a…

Using Mass Spectrometry and 2D-NMR to characterize biomass-derived liquid and solid streams

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Mariefel Olarte

This proposal aims to generate a deeper understanding of bio-oils and lignin samples through characterization using advanced chromatography (LC and GC), mass spectrometry (MS) and nuclear magnetic…

Using Mass Spectrometry and 2D-NMR to characterize biomass-derived liquid and solid streams

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Mariefel Olarte

This proposal aims to generate a deeper understanding of bio-oils and lignin samples through characterization using advanced chromatography (LC and GC), mass spectrometry (MS) and nuclear magnetic…

Software Integration for Data Analysis of High-Resolution Liquid Chromatography Tandem Mass Spectra from Low Molecular Weight Organic Matter

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nikola Tolic

LC-MS-MS/MS datasets consists of hundreds, thousands and sometimes even hundreds of thousands precursor (MS) and fragmentation spectra (MS/MS). Automation of data analysis is an absolute necessity to…

Extracellular Research Thrust for EMSL SSA

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kirsten Hofmockel

This project will develop a conceptual framework that defines the biotic and abiotic mechanisms regulating the persistence of mineral associated organic matter (OM). The specific goals are to…

MICROBES PERSIST: SYSTEMS BIOLOGY OF THE SOIL MICROBIOME

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Soils store more carbon than the atmosphere and biosphere combined, yet the mechanisms that regulate soil C remain elusive. While plant roots are the initial source of carbon (C) that enters…

Software Development for High-Resolution Mass Spectrometry Analysis of Small Molecules: Enabling High-Throughput, Reproducible Data Processing and Annotation.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Yuri Eberlim de Corilo

Modern high-resolution mass spectrometry has dramatically increased the volume (distinct analytical techniques and polarity modes) and quality (higher resolving power, dynamic range, mass error…

Developing Calculation Methods to Precisely Measure Proteome Proteins and Their Post-Translational Modifications

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nikola Tolic

In this proposed work we intend to: 1) Make an UStag caluclation algorithm into a high-throughput format that would be user friendly fo rthe general proteome community; 2) Create a human post…

The Effects of Earthworms on Ecosystem Multifunctionality (E3M)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christer Jansson

In a collaborative endeavor between EMSL and the CNRS Ecotron facility in Monpellier, France, we are launching a pilot project, E3M, to explore how Ecotron’s Macrocosm platform with ecosystem-scale…

An integrated workflow for identification and quantitation of intact phosphoproteins

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Multisite protein modifications involving phosphorylation alone or in combination with other post-translational modifications (PTMs) play an essential role in regulating protein activity. Hence,…

1000 Soils Research Campaign

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Hess

Project type

MONet

To more fully understand the cycling of carbon, carbon-based nutrients and key elements from the molecular to basin scale across variations in soil chemistry, the Environmental Molecular Sciences…

Projects