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Paul Ellis

Institution
Pacific Northwest National Laboratory

Projects

Investigation of the role of Mg2+ in DNA repair proteins APE1, Pol ?, and FEN1

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Paul Ellis
Project type
Capability Research
We propose to utilize unique capabilities within the EMSL (low temperature solid-state NMR and the mpp2 super computer) to investigate Mg2+ binding to two DNA repair proteins Pol ? and APE1. In the…

Invesitgation of the role of Mg2+ in DNA repair proteins

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
The chemistry central to the function of the DNA repair proteins APE1, Pol b and FEN1 is the chemistry of water activated by a magnesium ion. Further, Mg2+ also facilitates the organization of the…

The Mechanism of Action of Carbonic Anhydrase and LpxC

Lead Institution
University of Michigan
Principal Investigator
Carol Fierke
Project type
Capability Research
Zinc binding sites in proteins play diverse roles, including enhancement or inhibition of catalysis, stabilization and assembly of protein structures, and regulation of metal concentration. Further…

Solid-State 67Zn NMR of Synthetic Metalloprotein Models

Lead Institution
Columbia University
Principal Investigator
Gerard Parkin
Project type
Capability Research
It is the invention of this proposal to describe studies that will be undertaken in order to aid our understanding of the structures and mechanisms of zinc enzymes. Such knowledge will be obtained…

43Ca NMR Spectroscopy of Ca2+ Dependent Proteins and models

Lead Institution
Pacific Northwest National Laboratory
Principal Investigator
Paul Ellis
Project type
Large-Scale EMSL Research
We are exploring the application and utility of low temperature solid-state NMR methods to the observation of 43Ca in Ca2+ dependent proteins. Our first application is to the protein calbindin D9k …

Investigation of the role of Mg2+ in DNA repair proteins APE1, Pol ?, and FEN1

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
Nuclear DNA is continuously damaged by endogenous sources, such as the reactive free radicals produced during oxidative metabolism, or by exogenous sources, such as ionizing radiation. It has been…

Investigation of the role of Mg2+ in DNA repair proteins APE1, Pol ?, and FEN1

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
Nuclear DNA is continuously damaged by endogenous sources, such as the reactive free radicals produced during oxidative metabolism, or by exogenous sources, such as ionizing radiation. It has been…

Magnesium NMR of DNA Repair Proteins

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Exploratory Research
The chemistry central to the function of the DNA repair proteins apurinic/apyrimidic endonuclease 1 (APE1) and polymerase beta (pol beta) is the chemistry of water activated by a magnesium ion. …

Investigation of the role of Mg2+ in DNA repair proteins APE1, Pol ?, and FEN1

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
Nuclear DNA is continuously damaged by endogenous sources, such as the reactive free radicals produced during oxidative metabolism, or by exogenous sources, such as ionizing radiation. It has been…

Invesitgation of the role of Mg2+ in DNA repair proteins

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Andrew Lipton
Project type
Capability Research
The chemistry central to the function of the DNA repair proteins APE1, Pol b and FEN1 is the chemistry of water activated by a magnesium ion. Further, Mg2+ also facilitates the organization of the…