Robert Young

Electrochemical 19F Labelling of Technical Lignin for Enhanced NMR Analysis

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Robert Young

Nuclear Magnetic Resonance (NMR) is a powerful tool to analyze the structure of lignin, however the complexity of this natural mixture hinders interpretation due to substantial signal overlap in…

A User Interface Framework for Rigorous and Automated NMR Metabolomics Workflows

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Robert Young

NMR is powerful tool to study complex systems due to its non-destructive, quantitative, and global profiling capability making it an indispensable part of metabolomics research. EMSL's NMR…

Integrated multi-modal approach for structure and function determination of lignin-forming protein complexes isolated from plants

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

We propose to apply an integrated multi-modal structural biology approach to investigate a biosynthetic protein complex in plants that is hypothesized to organize and guide lignin biosynthesis via…

Chemically-Rich Structure and Dynamics in the Active Site of Tryptophan Synthase

Lead Institution

University of California, Riverside

Principal Investigator

Leonard Mueller

Project type

Limited Scope

Our goal is to understand the molecular basis for reaction specificity in pyridoxal-5'-phosphate (PLP) dependent enzymes, focusing on the 143 kDa, PLP-dependent enzyme tryptophan synthase (TS),…

Structural characterization of polycyclopropanated high energy biofuels produced by fermentation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Cort

This project will use NMR spectroscopy and mass spectrometry to elucidate structures and profile mixtures of novel fatty acids produced by engineered microorganisms.

Semi-Automated NMR Metabolite Profiling Software

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Javier Flores

NMR is powerful tool to identify and quantify metabolites in biological systems, and EMSL's NMR instrumentation makes automated data acquisition routine. However, the key downstream data…

Semi-Automated NMR Metabolite Identification via Machine Learning

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Javier Flores

Current EMSL metabolomics capabilities enable automated Nuclear Magnetic Resonance (NMR) data acquisition, yet downstream processing of these data is manually intensive and time-consuming. The…

EMSL Science Overview

Robert Young

Projects

Electrochemical 19F Labelling of Technical Lignin for Enhanced NMR Analysis

A User Interface Framework for Rigorous and Automated NMR Metabolomics Workflows

Integrated multi-modal approach for structure and function determination of lignin-forming protein complexes isolated from plants

Chemically-Rich Structure and Dynamics in the Active Site of Tryptophan Synthase

Structural characterization of polycyclopropanated high energy biofuels produced by fermentation

Semi-Automated NMR Metabolite Profiling Software

Semi-Automated NMR Metabolite Identification via Machine Learning