Rosalie Chu | Environmental Molecular Sciences Laboratory

Increased confidence, coverage, and characterization of organic matter through online liquid chromatography hybrid FT-Orbitrap-21T ICR mass spectrometry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

William Kew

Direct infusion (DI) mass spectrometry analysis of organic matter (OM) is one of EMSL’s primary workflows. It is a powerful and popular means to characterize complex environmental samples. However,…

AI-enabled processing of multimodal data to expand 2D chemical information to 3D space

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Maruti K. Mudunuru

This project will develop a user-friendly workflow to process EMSL’s multi-modal data using AI segmentation algorithms in Fiji software (already available on EMSL OnDemand). This multi-modal data…

Deep spatial metabolomics for confidential molecular identification

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Marija Velickovic

This project will improve metabolite identification of plant systems and microbial communities by employing an LC separation for every coordinate probed. Current imaging techniques for metabolite…

CPTAC Proteome Characterization Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Proteome Characterization Center (PCC) is to comprehensively characterize human tumor samples provided by the National Cancer Institute (NCI), and to integrate the…

Expanding metabolomics sample preparation with new desalting protocols for enhanced molecular coverage

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

William Kew

Metabolomics workflows are a cornerstone of EMSL’s user program. Routinely, samples are received which are rich in inorganic components such as salts, trace metals, and minerals. These ‘salts’ impede…

Network analysis of soil organic matter molecular chemistry to enable more robust modeling of C cycle along ecosystem gradients

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Hess

We will explore graphical network analysis of ultra-high resolution FTICR-MS datasets as the first step to derive a quantitative measure of soil organic matter quality that can inform microbially…

Understanding Biases in Natural Organic Matter FTICR Mass Spectrometry to More
Accurately Model Complex Environmental Systems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Rosalie Chu

Characterization of natural organic matter (NOM) by direct infusion (DI) Fourier transform mass spectrometry (FTMS) yields rich and complex information about environmental systems. However, the…

The role of priming effects on the conversion of blue carbon to CO2 in the coastal zone

Lead Institution

University of Florida

Principal Investigator

Thomas Bianchi

Project type

FICUS Research

"Blue carbon," or organic carbon (OC) derived from and stored in coastal and marine environments, is recognized as an important OC sink within the global carbon cycle, particularly in…

Acquisition-Time Quality Control Workflow for FT-ICR MS Complex Mixture Analysis

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Yuri Eberlim de Corilo

High throughput complex mixture analysis is hindered by challenges in automating data acquisition, namely ensuring that the data generated is of consistently high quality as required for automatic…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Extractions of Soil Organic Matter Using High Temperature and Pressure by Way of Commercial Espresso Machine

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jason Toyoda

Soil contains one of the largest carbon pools on earth in the form of organic matter, which is a highly complex mixture. Characterization of organic matter can inform biogeochemical processes…

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

High Confidence Characterization of Organohalogen & Organometallic Compounds by Orthogonal LC-ICP and LC-HR Mass Spectrometry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Yuri Eberlim de Corilo

Interactions between metals, halogens, and other elements are critical regulators of organic matter transport, persistence, and toxicity. Still, they remain poorly characterized due to the samples&…

Atmospheric Soil Organic Particles

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Recent discovery of atmospheric soil organic particles (ASOP), emitted through previously unrecognized atmosphere-land-surface interactions, suggests that ASOP may have a widespread impact over a…

Integrating mineral-associated organic matter (MAOM) colloid characteristics into dissolved organic matter (DOM) representation in Earth system models

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ravi Kukkadapu

We propose developing a workflow to generate colloids from operationally defined DOM solutions using aerosol-based techniques, then develop a spectroscopic/microscopic capability pipeline to…

Characterizing a tripartite plant - fungus - bacteria symbiosis important to peatland carbon and nitrogen cycling

Lead Institution

Oak Ridge National Laboratory

Principal Investigator

David Weston

Project type

Large-Scale EMSL Research

The importance of plant-microbiome systems on terrestrial carbon and nitrogen processes is perhaps most pronounced in Sphagnum dominated ecosystems, which occupy 3% of the Earth's land surface…

1000 Soils Research Campaign

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Hess

Project type

MONet

To more fully understand the cycling of carbon, carbon-based nutrients and key elements from the molecular to basin scale across variations in soil chemistry, the Environmental Molecular Sciences…

Using Mass Spectrometry and 2D-NMR to characterize biomass-derived liquid and solid streams

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Mariefel Olarte

This proposal aims to generate a deeper understanding of bio-oils and lignin samples through characterization using advanced chromatography (LC and GC), mass spectrometry (MS) and nuclear magnetic…

High-throughput native mass spectrometry as experimental validation for in silico drug design

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Enzyme functions can often be modulated via interaction with small molecules. For example, inhibitors are actively sought in the pharmaceutical industry to block protein targets and treat diseases…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Using Mass Spectrometry and 2D-NMR to characterize biomass-derived liquid and solid streams

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Mariefel Olarte

This proposal aims to generate a deeper understanding of bio-oils and lignin samples through characterization using advanced chromatography (LC and GC), mass spectrometry (MS) and nuclear magnetic…

A scalable mass spectrometry platform for proteome mapping of brain tissues

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

The brain is the most complex organ in the mammalian body. Bulk analysis obscures heterogeneity of cell types present even in the smallest brain regions. Multi-omics single-cell resolution 3D…

Genetically-Engineered, Nanoscale, Biosilica-Immobilized Functional Materials

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Guri Roesijadi

The next generation materials for the deployment of antibodies and receptors with the enhanced stability needed for real-world applications will be three dimensional nanosystems with multiscale…

Fuel Reduction Techniques as Effective Forested Watershed Management Practices against Wildfire: Drinking Water Quality Aspects

Lead Institution

The Chinese University of Hong Kong

Principal Investigator

Alex Chow

This research project will investigate the consequences of different fuel reduction techniques, as watershed management practices against wildfire, on the exports of dissolved organic matter (DOM)…

Building an automation-ready multimodal workflow for comprehensive 3D characterization of intact soil structures.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Odeta Qafoku

3-dimensional (3D) characterization of intact soil structures (i.e. macro and microaggregates) will provide understandings on how soil physical, mineralogical, biotic heterogeneities control long…

Multi-Omics Evaluation of Tripartite Associations in Red Alder Roots: Integrated Transcriptomics, Proteomics, Metabolomics and Metabolite Imaging of Red Alder Symbiotic Associations in Root Tissue

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

This proposal presents the unique opportunity to conduct the first ever integrated multi-omics and imaging evaluation of the Alnus rubra (red alder tree)/Frankia alni (bacteria)/Alpova diplophloeus …

Elucidating synergistic plant-bacteria metabolic pathways for alleviating iron deficiency

Lead Institution

Oregon State University

Principal Investigator

Rene Boiteau

Nutrient mobilization from soil minerals is critical for plant growth, particularly in marginal lands with high pH soils or low phosphate availability. One way that rhizospheric bacteria enhance…

Online, real-time instrument parameter optimization for FT-ICR mass spectrometry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

William Kew

Optimal and efficient use of FT-ICR mass spectrometers is a critical step to high-throughput and automated analyses, as well as for ensuring the highest fidelity of data and scientific rigor. This…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Utilizing LESA to characterize cell-to-cell communication through the secreted metabolomes of microbial communities

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Inter-cellular communication is a complex process that plays a pivotal role in the microbiome and is essential for our understanding of microbial dynamics and functional roles. Teasing apart…

Rhizosphere Underground:
Unraveling the Role of Microbes in Stabilizing Carbon Pools in Soils

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Alice Dohnalkova

Microorganisms are fundamental to biogeochemical cycling, as microbial metabolism significantly contributes to the regulation of terrestrial carbon and nitrogen cycling. However, the impact of the…

Extracellular Research Thrust for EMSL SSA

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kirsten Hofmockel

This project will develop a conceptual framework that defines the biotic and abiotic mechanisms regulating the persistence of mineral associated organic matter (OM). The specific goals are to…

Advancing soil C characterization using HRMS and NMR

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Hess

Soil organic matter consists of diverse and complex mixture of hundreds to thousands biological and metabolic products that are poorly characterized using current techniques. We seek to develop a…

Identifying and Quantifying Sources of Variability in FTICR

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Allison Thompson

Many EMSL users are requesting Fourier transform ion cyclotron resonance (FTICR) soil organic matter (SOM) data. However, there are many circumstances where results are unexpected and experts…

Development of an in situ multimodal mass spectrometry imaging approach for studying the rhizosphere: from revealing activation of metabolic pathways to elucidating nanoscale exchanges

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Christopher Anderton

Gaining spatial information of biochemical interactions that occur within the rhizosphere and other subsurface terrestrial ecosystems is an area of major interest. Through bulk extraction methods, we…

CPTAC Proteome Characterization Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Proteome Characterization Center (PCC) is to integrate genomic information with proteomic technologies to comprehensively characterize the proteome and post…

High Resolution and Mass Accuracy Capability (HRMAC) Development Project

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Koppenaal

Access to other FTMS instrumentation is important o successfully and rapidly implement first science applications, to facilitate parallel testing of spectrometer subsystems, and to perform cross…

Software Development for High-Resolution Mass Spectrometry Analysis of Small Molecules: Enabling High-Throughput, Reproducible Data Processing and Annotation.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Yuri Eberlim de Corilo

Modern high-resolution mass spectrometry has dramatically increased the volume (distinct analytical techniques and polarity modes) and quality (higher resolving power, dynamic range, mass error…

Atmospheric Soil Organic Particles

Lead Institution

Purdue University

Principal Investigator

Alexander Laskin

Recent discovery of atmospheric soil organic particles (ASOP), emitted through previously unrecognized atmosphere-land-surface interactions, suggests that ASOP may have a widespread impact over a…

Chromatographic Approaches for Functional Natural Organic Matter Characterization

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kent Bloodsworth

Electrospray ionization (ESI), coupled to high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS), is the de facto standard for complex natural mixture analysis. Our…

Projects