Rui Zhao

CPTAC Proteome Characterization Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Proteome Characterization Center (PCC) is to comprehensively characterize human tumor samples provided by the National Cancer Institute (NCI), and to integrate the…

Systematic Characterization of Protein Glycosylation of Bacteria Cell Surface Proteins

Lead Institution

University of Oklahoma

Principal Investigator

Si Wu

The overall goal of this proposal is to develop an integrated top-down and bottom-up approach for bacterial glycoprotein profiling assisted by accurate glycan structure identification, which will be…

Targeted proteomics technology for accurate quantitative single-cell proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Multi-omics characterization of a broad spectrum of small subpopulations of cells between tumors and within individual tumors at the single-cell resolution is crucial to achieve understanding of a…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Center for Application of Advanced Clinical Proteomic Technologies for Cancer (NCI U24; 59963)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Richard Smith

The overall objective of the PNNL Clinical Proteome Characterization Center (PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype using detailed…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

High-Throughput Multidimensional Bioseparations for Next-Generation Proteomics

Lead Institution

Brigham Young University

Principal Investigator

Ryan Kelly

Mass spectrometry (MS)-based analysis of complex biological samples is essential for biological research including the study of microbiomes. The quality of the analysis is determined not only by the…

Characterization of the SATB1 phosphorylations upon ionization radiation

Lead Institution

Lawrence Berkeley National Laboratory

Principal Investigator

Terumi Kohwi-shigematsu

A major goal of my laboratory is to understand the molecular signaling mechanisms involving nuclear architectural protein, SATB1. SATB1 is known to be a global gene regulator by organizing higher…

CPTAC Proteome Characterization Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Proteome Characterization Center (PCC) is to integrate genomic information with proteomic technologies to comprehensively characterize the proteome and post…

Characterization of Signaling Events Induced by Low Dose Ionizing Radiation

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

David Stenoien

Project type

Scientific Partner

Exposure to low doses of radiation has the potential to adversely affect human health but the actual health risks and the underlying molecular mechanisms associated with these risks are still poorly…

In-Depth Proteome Mapping of the Tumor Microenvironment with Single-Cell Resolution

Lead Institution

Brigham Young University

Principal Investigator

Ryan Kelly

The development of effective therapies for cancer requires a deep molecular understanding of tumor heterogeneity by advanced omics technologies with spatially resolved measurements. Unfortunately,…

MITOTIC REGULATION OF THE NUCLEAR PORE COMPLEX PROTEIN INTERACTION NETWORK

Lead Institution

The Ohio State University

Principal Investigator

Stephen Osmani

Project type

Large-Scale EMSL Research

Eukaryotic cells segregate DNA equally to daughter cells during mitosis. Although mitosis is well characterized, it is poorly understood at the molecular level. The most extensive and least…

Joining Systems Biology and Bioimaging on Same Cells

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

We plan to develop a new capability at EMSL for coupling bioimaging and systems biology analysis of the same exact cells. The use of the highly simplified and smallest free-living eukaryote…

Collaborative Research Projects Supported by the NCRR P41 Resource Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

David Camp

Project type

Large-Scale EMSL Research

The Proteomics Resource Center for Integrative Biology at PNNL was established in 2003 and builds on the unique instrumentation and the infrastructure provided by PNNLs EMSL facility. The mission of…

CPTAC Proteome Characterization Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Proteome Characterization Center (PCC) is to integrate genomic information with proteomic technologies to comprehensively characterize the proteome and post…

The Measurements for Nano-tip'ID

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Rui Zhao

Project type

Exploratory Research

To find the best way for pull the nano-tip using P-2000 Micropipette Puller Capillary ID: 30um and 50um Conditions: With water and Without water.

Development and Implementation of Online Separation Techniques Coupled to Native Mass Spectrometry for Characterization of Endogenous Proteins Extracted from Biological Matrices

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Recent developments of state-of-the-art mass spectrometry (MS) instrumentation at EMSL, including the 21T Fourier Transform Ion Cyclotron Resonance (FTICR) MS, have enabled high resolution and high…

An integrated workflow for identification and quantitation of intact phosphoproteins

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Multisite protein modifications involving phosphorylation alone or in combination with other post-translational modifications (PTMs) play an essential role in regulating protein activity. Hence,…

Development of a functional proteomics workflow for characterization of glycoproteins

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Glycosylation is a diverse family of post-translational modification (PTM) that affects protein structure/function but is not directly encoded in the genome, and represents significant challenge for…

High-Throughput Proteomics: Applications and Development (PNNL Scope #40601 and #43488)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Gordon Anderson

This project involves the application and development of high-throughput proteomics and quantitative measurement capabilities within biological systems. More so, application measurements will be…

A streamlined platform for phosphoproteome mapping of human tissues

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Recent technological advances in genomics, transcriptomics, and proteomics allow for rapid generation of comprehensive 3D-human tissue maps for biomolecules DNAs, RNAs, and proteins at the single…

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

David Camp

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

A scalable mass spectrometry platform for proteome mapping of brain tissues

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

The brain is the most complex organ in the mammalian body. Bulk analysis obscures heterogeneity of cell types present even in the smallest brain regions. Multi-omics single-cell resolution 3D…