Thomas Fillmore

Universal internal standard for reproducible accurate quantification of exosomal proteins

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Pancreatic cancer is currently the third leading cause of cancer related to death in the USA with a 5- year survival rate of <5%. Most potentially curable patients present with unresectable…

Quantitative Proteomics-based Prostate Cancer Prediction Models for African American and Caucasian American Patients

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

In this research project, the Pacific Northwest National Laboratory (PNNL) research team will support the project by conducting quantitative proteomics analysis using mass spectrometry to identify…

Multiplex Mass Spectrometric Protein Assays for Obesity

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

The prevalence of overweight and obesity has increased dramatically in recent decades in both children and adults, reaching an epidemic proportion. In order to gain new insights into the…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Urinary Proteome Monitoring for Transplant Injury

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

David Camp

We propose to achieve the objective of identifying novel urinary biomarkers for selected categories of renal transplant injury in two phases. We will first employ a discovery phase to comprehensively…

Cancer Moonshot - Framingham

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Cancer Moonshot -Framingham project is to continue finishing analysis of ~1300 serum samples with applying multiplex quantitative LC-SRM-MS assays for comprehensive, quantitative…

Center for Application of Advanced Clinical Proteomic Technologies for Cancer (NCI U24; 59963)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Clinical Proteome Characterization Center (PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype using detailed…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Multi-marker Urine Test for Detection of Aggressive Prostate Cancer

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

In prostate cancer disease management over-diagnosis and over-treatment have increasingly become major public concerns. Prostate cancer is primarily detected by serum PSA screening followed by a…

Center for Application of Advanced Clinical Proteomic Technologies for Cancer (NCI U24; 59963)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Richard Smith

The overall objective of the PNNL Clinical Proteome Characterization Center (PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype using detailed…

Pathway Discovery, Validation and Compound Identification for Alzheimer's Disease

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vladislav Petyuk

The overall goal of the proposed study is the discovery and preclinical validation of novel targets associated with molecular processes that lead to cognitive decline, the primary outcome of most AD…

Advancing soil C characterization using HRMS and NMR

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Hess

Soil organic matter consists of diverse and complex mixture of hundreds to thousands biological and metabolic products that are poorly characterized using current techniques. We seek to develop a…

Elucidation of the Roles of Diazotrophic EndoPopulus: Endophyte Communities in Promoting Productivity and Resilience of Populus through Systems Biology Approaches (EndoPopulus)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Amir Ahkami

The plant microbiome, including microbial endophytes living within plants and the epiphytes living on the plant surface, has a profound influence on the productivity and resilience of the host plant…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Cancer Moonshot - Framingham

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Cancer Moonshot -Framingham projects is to perform multiplexed, quantitative LC-MS analysis of longitudinal serum samples associated with head and neck cancer, B-cell lymphoma,…

Agile BioFoundry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jon Magnuson

This proposed EMSL Resource Owner project, is a sub-project supporting two DOE-EERE-BETO supported PNNL projects: FY16-17 four-national laboratory “Agile BioFoundry” (ABF) seed project and a larger…

A streamlined platform for phosphoproteome mapping of human tissues

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Recent technological advances in genomics, transcriptomics, and proteomics allow for rapid generation of comprehensive 3D-human tissue maps for biomolecules DNAs, RNAs, and proteins at the single…

Developing a strategy to identify & validate pharmacodynamic kinase inhibitorbiomarkers

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Keqi Tang

The primary goal of the project is to develop mass spectrometry (MS) based assays capable of globally quantifying changes in kinase-specific phosphorylation stoichiometry. The specific work related…

Targeted proteomics technology for accurate quantitative single-cell proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Multi-omics characterization of a broad spectrum of small subpopulations of cells between tumors and within individual tumors at the single-cell resolution is crucial to achieve understanding of a…

Pathway Discovery, Validation and Compound Identification for Alzheimer's Disease

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vladislav Petyuk

The overall goal of the proposed study is the discovery and preclinical validation of novel targets associated with molecular processes that lead to cognitive decline, the primary outcome of most AD…

Agile BioFoundry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Kristin Burnum-Johnson

This proposed EMSL Resource Owner project, is a sub-project supporting two DOE-EERE-BETO supported PNNL projects: FY16-17 four-national laboratory “Agile BioFoundry” (ABF) seed project and a larger…

Multi-marker Urine Test for Detection of Aggressive Prostate Cancer

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

In prostate cancer disease management over-diagnosis and over-treatment have increasingly become major public concerns. Prostate cancer is primarily detected by serum PSA screening followed by a…

Pathway Discovery, Validation and Compound Identification for Alzheimer's Disease

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vladislav Petyuk

The overall goal of the proposed study is the discovery and preclinical validation of novel targets associated with molecular processes that lead to cognitive decline, the primary outcome of most AD…

Cancer Moonshot - Framingham

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Cancer Moonshot -Framingham project is to apply multiplex quantitative LC-SRM-MS assays for comprehensive, quantitative analysis of longitudinal serum samples associated with head…

Metabolomics of programmed cell death

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jennifer Kyle

Lipidomics is the study of cellular lipids to understand the function and structure of biological systems. The interaction of lipids with other cellular components, including proteins and…

Agile BioFoundry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

This proposed EMSL Resource Owner project, is a sub-project supporting two DOE-EERE-BETO supported PNNL projects: FY16-17 four-national laboratory “Agile BioFoundry” (ABF) seed project and a larger…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Pathway Discovery, Validation and Compound Identification for Alzheimer's Disease

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vladislav Petyuk

The overall goal of the proposed study is the discovery and preclinical validation of novel targets associated with molecular processes that lead to cognitive decline, the primary outcome of most AD…

Projects