Thomas Metz

Discovery and Biological Signatures of Microbiome-Derived Xanthohumol (XN) Metabolites and their Role in Ameliorating Inflammatory Bowel Disease (IBD)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

The limitations of current therapies for treatment of inflammatory bowel disease (IBD) underscore the need for new therapeutic modalities that both target the inflamed gut and the microbial dysbiosis…

Mammary and milk microbiomes and metabolomes - Understanding early variation and impacts on risk for mammary inflammation and mastitis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Exclusive breastfeeding for at least 4 mo is considered the optimal form of nutrition for most newborns, yet many women experience substantial roadblocks in meeting this goal. One of the most common…

CPTAC Proteome Characterization Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Proteome Characterization Center (PCC) is to comprehensively characterize human tumor samples provided by the National Cancer Institute (NCI), and to integrate the…

Advanced Proteomics and Metabolomics Studies of Type 1 Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Project type

Exploratory Research

In this four year two phase (R21/R33) project we will apply advanced proteomic and metabolomic nanoflow liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry technologies…

NMR-based metabolomics of the enteropathogens Salmonella and Yersinia

Lead Institution

Washington State University Tri-Cities

Principal Investigator

Kathleen McAteer

Project type

Large-Scale EMSL Research

The goal of this research is to analyze and reconstruct intercellular and intracellular networks in Salmonella and Yersinia using multiple types of -omics data. Although we hope to gain a more…

Development of an Integrated EMSL MS and NMR Metabolic Flux Analysis Capability In Support of Systems Biology: Test Application for Biofuels Production

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Isern

An important element in the production of biofuels is the metabolic engineering process required to maximize the production of renewable biofuel molecules from fermentable substrates. Biofuel…

Undiagnosed Diseases Network Metabolomics Core

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

A state of the art metabolomics core will be a vital part of the Undiagnosed Diseases Network (UDN). However, in order for the UDN to realize it’s the full potential it is essential that the…

OHSU-PNNL NORTHWEST CO-LABORATORY FOR INTEGRATED OMICS

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Karin Rodland

An essential component of the OHSU-PNNL Northwest CoLaboratory for Integrated Omics (CoLab) is the collaborative pursuit of joint research projects that leverage the compelling biomedical model…

Identification of Metabolite Toxins

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

David Wunschel

This project will collect data on ion-mobility, accurate mass and compound fragmentation data using an ESI-IMS-MS/MS system for a number of metabolite toxins and structurally similar compounds. This…

Pacific Northwest Advanced Compound Identification Core

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

The capability to chemically identify thousands of metabolites and other chemicals in clinical samples will revolutionize the search for environmental, dietary, and metabolic determinants of disease…

Advanced Proteomics and Metabolomics Studies of Type 1 Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

In this four year two phase (R21/R33) project we will apply advanced proteomic and metabolomic nanoflow liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometry technologies…

Evaluation of the specificity and early diagnostic value of novel peptide biomarkers of Type 1 Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Type 1 diabetes (T1D) results from autoimmune destruction of insulin-producing pancreatic B-cells triggered by complex interactions of environmental factors in genetically predisposed individuals. …

Type 1 Diabetes Mellitus: Correlating the Gastrointestinal Microbiome, Urinary Proteome and Metabolome

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Type 1 diabetes mellitus (T1D) is characterized by autoimmune destruction of the insulin producing ?-cells of pancreatic islets. It is believed that T1D results from complex interactions between…

Deciphering Microbial Communication Through Metabolites

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This project will establish a Metabolomics Center at PNNL that will develop the advanced measurement capabilities necessary to define the metabolic processes of microbiomes and determine how these…

Development of high-throughput metabolomics technologies: Application to studying the flowering time in Arabidopsis thaliana

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

David Hoyt

The future trajectory of metabolomics and its applications largely depends on the analytical capabilities of NMR and MS and their combination. Although the use of both NMR and MS methods is…

Modeling Host Responses to Understand Severe Human Virus Infections

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This comprehensive and highly integrated systems biology program seeks to delineate the complex host responses that determine the outcome of infections with potentially lethal viruses. To achieve…

Prediction of soil microbiome phenotypic responses to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

Development of Metabolomics for Biomarker Discovery

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Project type

Exploratory Research

Metabolomics is a holistic approach to the study of a biological system’s metabolite complement (often generalized to include essentially all small molecules), and its practice is anticipated to…

A Systems Biology Approach to Emerging Respiratory Viral Diseases

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This Systems Biology Program will comprehensively analyze and model the molecular and cellular events and pathogen-host interactions that occur during the course of respiratory virus infection. Our…

Metabolomics of Mixed Microbial Communities: Understanding the Influence of Gut Microbiota

Lead Institution

Arizona State University

Principal Investigator

Rosa Krajmalnik-Brown

Understanding the complex relationships in mixed microbial communities and host-microbiome interactions directly addresses the EMSL Science Theme of Biological Interactions and Dynamics: “Developing…

OHSU-PNNL NORTHWEST CO-LABORATORY FOR INTEGRATED OMICS

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Karin Rodland

An essential component of the OHSU-PNNL Northwest CoLaboratory for Integrated Omics (CoLab) is the collaborative pursuit of joint research projects that leverage the compelling biomedical model…

Comprehensive comparison of taxonomic composition and metabolic functioning in oxygenic and anoxygenic microbial mats of Yellowstone hot springs

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This proposal focuses on comparing a rare, but evolutionarily important, modern laminated microbial mat formed exclusively by anoxygenic photosynthetic microorganisms and a well-studied mat formed by…

Prediction of soil microbiome phenotypic responses to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jason McDermott

The goal of this project is to attain a systems level understanding of the soil microbiome and its phenotypic response to changing moisture through a tractable, spatially explicit examination of the…

Mammary and milk microbiomes and metabolomes - Understanding early variation and impacts on risk for mammary inflammation and mastitis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Exclusive breastfeeding for at least 4 mo is considered the optimal form of nutrition for most newborns, yet many women experience substantial roadblocks in meeting this goal. One of the most common…

Title: Advanced Proteomics and Metabolomics Studies of Type 2 Diabetes and Pre-Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Abstract: In this five year (R21/R33) project we will apply advanced proteomic and metabolomic nanoflow liquid chromatography-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Developing Signatures that Relate Fecal Microbiome Characteristics with Patient Phenotype

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Isern

The exploration of the human microbiome is currently an area of keen interest to NIH and biotech firms, and has been explored by many researchers using either NMR or MS metabolomics or 16S rDNA…

Predictive Modeling of Viral Pathogenesis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The interactions between a host and a pathogen are complex, representing a molecular interplay that results in either health or disease as an outcome. Understanding the nuances of these interactions…

Molecular Phenotyping of Brachypodium to Provide Metabolic and Functional Linkages to the Plant-Atmosphere-Soil-Systems (iPASS) Model

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

This proposal aims to provide the fundamental molecular characterization necessary to develop a model which ultimately links phenotypic measurements to the atmospheric processes and soil…

Undiagnosed Diseases Network Metabolomics Core

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

A state of the art metabolomics core will be a vital part of the Undiagnosed Diseases Network (UDN). However, in order for the UDN to realize it’s the full potential it is essential that the…

Discovery and Biological Signatures of Microbiome-Derived Xanthohumol (XN) Metabolites and their Role in Ameliorating Inflammatory Bowel Disease (IBD)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

The limitations of current therapies for treatment of inflammatory bowel disease (IBD) underscore the need for new therapeutic modalities that both target the inflamed gut and the microbial dysbiosis…

Title: Advanced Proteomics and Metabolomics Studies of Type 2 Diabetes and Pre-Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Abstract: In this five year (R21/R33) project we will apply advanced proteomic and metabolomic nanoflow liquid chromatography-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry…

Type 1 Diabetes Mellitus: Correlating the Gastrointestinal Microbiome, Urinary Proteome and Metabolome

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Type 1 diabetes mellitus (T1D) is characterized by autoimmune destruction of the insulin producing ?-cells of pancreatic islets. It is believed that T1D results from complex interactions between…

A Molecular Atlas of the Developing Lung

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Richard Corley

The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our understanding of alveologenesis is a critical step…

Metabolic Flux Coupling in Microbial Consortia: Demystifying Metabolic Exchanges across Species

Lead Institution

University of Tromso - The Arctic University of Norway

Principal Investigator

Hans Bernstein

Project type

Large-Scale EMSL Research

We propose to employ EMSL's state-of-the-art metabolomics capabilities to gain a predictive understanding of how compartmentalized metabolic reactions are coordinated across carbon and energy…

FSFA: MOSAIC—Metabolic and Spatial Interactions in Communities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Errol Robinson

This EMSL user proposal is to support the MOSAIC DOE BER funded FSFA at PNNL. The primary activities involving EMSL are a set of BSSD demonstration tasks focusing on key science questions and related…

OHSU-PNNL NORTHWEST CO-LABORATORY FOR INTEGRATED OMICS

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

An essential component of the OHSU-PNNL Northwest CoLaboratory for Integrated Omics (CoLab) is the collaborative pursuit of joint research projects that leverage the compelling biomedical model…

Multi-platform 'Omics Analysis to Determine TB Vaccine Correlates

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jennifer Kyle

In recent years, mass spectrometry-based integrated multi-omics analyses have allowed for powerful interrogations of patient samples, and the identification of correlates of risk for tuberculosis…

Cyanobacteria Membrane Biology Grand Challenge: Systems Analysis of the Dynamics of Membrane Architecture, Composition, and Function- Proteomic, Metabolomic, and Metallomic Characterization

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Project type

Grand Challenge

The strain Cyanothece sp. ATCC 51142, isolated from benthic waters off the coast near Galveston, Texas, is a unicellular strain approximately 4-7 &#956;m in diameter along the major axis. This…

Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

Develop tools for cell-wide measurement of metabolites and lipids, which, along with transcriptional data, will allow the construction of genome-scale metabolic models, as well as models of…

Optimizing Oil Production in Oleaginous Yeast by Cell-Wide Measurements and Genome-Based Models

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

The team at PNNL is using a combination of flow cytometry, microscopy and lipidomics to establish baseline cell biological and metabolite data on lipid production by the W29 strain of Yarrowia…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

FSFA: MOSAIC -- Metabolic and Spatial Interactions in Communities FY18 proposal

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Errol Robinson

This EMSL user proposal is to support the MOSAIC DOE BER funded FSFA at PNNL. The primary activities involving EMSL are a set of BSSD demonstration tasks focusing on key science questions and related…

Mapping Nutrient Flow through a Plant-Microbe Symbiont System using Stable Isotope Probing under Drought and Salinity Stress

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Kim Hixson

Red alder (Alnus rubra) is one of the fastest growing, commercially important, hardwood species for biomass/timber production in North America, and is also involved in a nitrogen-fixation symbiosis…

Does the brain differentially alter metabolic and immunologic programs in the bone marrow in a pathogen-specific manner?

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Sneha Couvillion

: A wide variety of pathogens present an acute survival challenge in humans, and our ability to rapidly and precisely respond to these pathogens is critical to our survival as a species. Although…

High Resolution 1H NMR Metabolomics Using Slow and Ultra Slow Magic Angle Spinning

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jian-zhi Hu

Project type

Large-Scale EMSL Research

Recently, we developed new so-called slow-MAS (magic angle spinning) NMR techniques that significantly enhance the spectral information in biofluids, intact excised tissues, organs and live small…

Biological Systems Interactions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

James Fredrickson

SPECIFIC AIMS: The overall objective of the proposed research is to acquire a predictive understanding of how microbial interactions impart stability, fitness, and functional efficiency to microbial…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Establishment of a Robust Method for Soil Metabolomics Analysis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vanessa Bailey

Project type

Limited Scope

The objective of this work is to adapt current evolving protocols for metabolomics analyses to soil samples. As chemical perturbations are primarily detected on a molecular level, the metabolome will…

BetaMarker: Biomarkers of Beta Cell Stress in Type 1 Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Type 1 diabetes (T1D) is generally accepted to result from impaired immune tolerance, leading to the autoimmune destruction of islet beta cells. However, the durable preservation of beta cell…

Projects