Tong Zhang

Integrative Multi-PTM Profiling by an Automated Shotgun Proteomics Coupled with Top-down Proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tong Zhang

Project type

Contracted Time

To use PTM data to understand cell signaling and profile molecular phenotypes, PNNL has made substantial strides in advancing phosphoproteome and thiol redox proteome profiling. However, the current…

Enhancing biopreparedness through a model system to understand the molecular mechanisms that lead to pathogenesis and disease transmission

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

The science of biopreparedness to counter biological threats hinges on understanding the fundamental principles and molecular mechanisms that lead to pathogenesis and disease transmission. Our vision…

Integrative Multi-PTM Profiling by an Automated Shotgun Proteomics Coupled with Top-down Proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tong Zhang

To use PTM data to understand cell signaling and profile molecular phenotypes, PNNL has made substantial strides in advancing phosphoproteome and thiol redox proteome profiling. However, the current…

Predicting adverse biological effects for chemicals of concern through integration of functional profiling with artificial intelligence/machine learning (AI/ML)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We will use thermal proteome profiling (TPP) to identify protein targets and pathways affected by exposure to a chemical of concern (COC). As a next step, we will use an artificial intelligence…

Universal internal standard for reproducible accurate quantification of exosomal proteins

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Pancreatic cancer is currently the third leading cause of cancer related to death in the USA with a 5- year survival rate of <5%. Most potentially curable patients present with unresectable…

Serum biomarkers for predicting type 1 diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Clinical type 1 diabetes (T1D) is preceded by an asymptomatic phase of the disease, which can be readily identified by serum islet autoantibodies(AAb). However, the T1D field is challenged by the…

A streamlined platform for phosphoproteome mapping of human tissues

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Recent technological advances in genomics, transcriptomics, and proteomics allow for rapid generation of comprehensive 3D-human tissue maps for biomolecules DNAs, RNAs, and proteins at the single…

A scalable mass spectrometry platform for proteome mapping of brain tissues

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

The brain is the most complex organ in the mammalian body. Bulk analysis obscures heterogeneity of cell types present even in the smallest brain regions. Multi-omics single-cell resolution 3D…

Targeted proteomics technology for accurate quantitative single-cell proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Multi-omics characterization of a broad spectrum of small subpopulations of cells between tumors and within individual tumors at the single-cell resolution is crucial to achieve understanding of a…

Structural profiling of the molecular interactome to define phenotype

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

John Melchior

The functional pathways that define phenotype are reflected by protein quinary structure; the transient encounters of proteins with other proteins, molecules, cofactors, and nucleic acids that…

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Projects