Skip to main content

Trevor Moser

Institution
Environmental Molecular Sciences Laboratory

Projects

Accelerating cryo-EM screening at EMSL by 25-fold

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
Cryo-Electron Microscopy (cryo-EM) is a powerful structural biology technique since it does not require crystallization (unlike X-ray), does not require ultrahigh purity (unlike NMR) and provides…

Project Title: Protein Structure Without Purification

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
Purification remains a major technical and temporal bottleneck for protein annotation especially for membrane, filamentous and heterocomplex assembling proteins. If a target protein in a mixed…

Structural study of full-length SARS-Cov-2 Spike proteins by Cryo-EM

Lead Institution
Oregon Health & Science University
Principal Investigator
Liman Zhang
As of February 25th, 2020 there were 80,146 confirmed cases of SARS-Cov-2 infection across the globe. However, no vaccine or drug is available to prevent or treat the infection. Spike protein of SARS…

Elucidating the structure of C. elegans TMC1 and TMC2

Lead Institution
Oregon Health & Science University
Principal Investigator
Eric Gouaux
Transmembrane channel-like (TMC) proteins are a unique family of channel-like proteins that are conserved from humans to C. elegans. Vertebrates express eight isoforms, two of which are essential for…

Determination of inner hair cell stereocilia widening mechanism

Lead Institution
Oregon Health & Science University
Principal Investigator
Peter Barr-Gillespie
Hair cells are the sensory cells of the inner ear, and use the mechanically sensitive hair bundle to detect sound and head movements. Bundles are composed of a hundred or so stereocilia, each of…

Visualizing pathways in cells for more accurate biodesign

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
In this Capability Development Focus project, we plan to acquire label-free whole cell three-dimensional (3D) tomograms of the two primary organisms of interest for the FY18 Subcellular SSA thrust…

Collaborative Access Team: Pacific Northwest Cryo-EM Center (PNCC)

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
PNNL will create and operate the Pacific Northwest Center for Cryo-EM (PNCC), a state-of-the-art national center for cryo-electron microscopy (Cryo-EM) that will rapidly and efficiently collect and…

Joining Systems Biology and Bioimaging on Same Cells

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
We plan to develop a new capability at EMSL for coupling bioimaging and systems biology analysis of the same exact cells. The use of the highly simplified and smallest free-living eukaryote…

Towards proteome-wide assessment of cellular landscapes

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
Ljiljana Pasa-Tolic
We will develop a novel workflow to characterize proteome-wide structural changes of cellular landscapes between different cell states (or cell types). The goal is to bridge the gap between cellular…

KATP channel structures

Lead Institution
Oregon Health & Science University
Principal Investigator
Show-Ling Shyng
ATP-sensitive potassium (KATP) channels couple cell metabolism to membrane excitability and are important for regulating hormone secretion, neuronal activity, vascular tone, and adaptive response to…

Cryo-EM studies of TRP channels

Lead Institution
Duke University
Principal Investigator
Seok-Yong Lee
Transient receptor potential (TRP) channels are a superfamily of calcium-permeable ion channels that are involved in diverse physiological processes. A subset of TRP channels that are known as the…

Dynamic TEM

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
Achieving a holistic, molecular-level understanding of the structure, function and mechanism of cellular machinery remains a formidable scientific challenge. While over 100,000 protein structures…

Tomography of Pseudarthrobacter chlorophenolicus

Lead Institution
Environmental Molecular Sciences Laboratory
Principal Investigator
James Evans
Subcellular structures and macromolecular complexes can be revealed through cryo electron tomography of whole cells. Pseudarthrobacter chlorophenolicus is a soil bacterium which has novel metabolic…

Structural Mechanism of Jordan's Syndrome

Lead Institution
University of Wisconsin, Madison
Principal Investigator
Yongna Xing
Protein phosphatase 2A (PP2A) is an essential and major serine/threonine phosphatase in all eukaryotic cells, and regulates multiple cellular processes and signaling events. Deregulation of PP2A and…

Imaging Transcriptional Complexes by CryoEM

Lead Institution
Emory University
Principal Investigator
Eric Ortlund
Nuclear receptors (NRs) sense changes in lipid metabolite levels to trigger specific patterns of gene regulation, which drive critical biological processes including metabolism, development, and…