Trevor Moser

Enhancing biopreparedness through a model system to understand the molecular mechanisms that lead to pathogenesis and disease transmission

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

The science of biopreparedness to counter biological threats hinges on understanding the fundamental principles and molecular mechanisms that lead to pathogenesis and disease transmission. Our vision…

Accelerating cryo-EM screening at EMSL by 25-fold

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Cryo-Electron Microscopy (cryo-EM) is a powerful structural biology technique since it does not require crystallization (unlike X-ray), does not require ultrahigh purity (unlike NMR) and provides…

Enhancing EMSL’s cryo-EM capability through a novel high pressure freezing workflow to improve throughput and sample quality

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Trevor Moser

Cryo-focused ion beam/scanning electron microscopy (cryo-FIB/SEM) is an essential sample preparation technique for creating thin sections (500nm or less) of cells and tissues for cryo-transmission…

Project Title: Protein Structure Without Purification

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Purification remains a major technical and temporal bottleneck for protein annotation especially for membrane, filamentous and heterocomplex assembling proteins. If a target protein in a mixed…

Structural studies of the bacterial ribosome: regulation of gene expression, stress tolerance and antibiotic resistance.

Lead Institution

Emory University

Principal Investigator

Christine Dunham

In every living organism, protein synthesis (translation) is catalyzed by a large, dynamic RNA-protein macromolecular complex called the ribosome. We use biochemical, structural and functional…

Advanced High Resolution and Dynamic in-situ TEM/STEM Observations of Materials Processes

Lead Institution

University of Liverpool

Principal Investigator

Nigel Browning

The first goal of this research program is to develop a robust method to quantify the atomic scale changes in structure, composition and bonding that occur at interfaces under a variety of…

Imaging Transcriptional Complexes by CryoEM

Lead Institution

Emory University

Principal Investigator

Eric Ortlund

Nuclear receptors (NRs) sense changes in lipid metabolite levels to trigger specific patterns of gene regulation, which drive critical biological processes including metabolism, development, and…

Advanced High Resolution and Dynamic in-situ TEM/STEM Observations of Materials Processes

Lead Institution

University of Liverpool

Principal Investigator

Nigel Browning

The first goal of this research program is to develop a robust method to quantify the atomic scale changes in structure, composition and bonding that occur at interfaces under a variety of…

Structural study of full-length SARS-Cov-2 Spike proteins by Cryo-EM

Lead Institution

Oregon Health & Science University

Principal Investigator

Liman Zhang

As of February 25th, 2020 there were 80,146 confirmed cases of SARS-Cov-2 infection across the globe. However, no vaccine or drug is available to prevent or treat the infection. Spike protein of SARS…

Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Elucidating the structure of C. elegans TMC1 and TMC2

Lead Institution

Oregon Health & Science University

Principal Investigator

Eric Gouaux

Transmembrane channel-like (TMC) proteins are a unique family of channel-like proteins that are conserved from humans to C. elegans. Vertebrates express eight isoforms, two of which are essential for…

Systems Biology Through an Integrated Multimodal Imaging and Analysis Framework

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Determination of inner hair cell stereocilia widening mechanism

Lead Institution

Oregon Health & Science University

Principal Investigator

Peter Barr-Gillespie

Hair cells are the sensory cells of the inner ear, and use the mechanically sensitive hair bundle to detect sound and head movements. Bundles are composed of a hundred or so stereocilia, each of…

Unraveling New Pathways for Carbon Capture to Enhance Lipid-Biomass Feedstocks

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Organisms have evolved a number of spatially localized processes to efficiently break down, absorb, and use necessary metabolites. Although progress has been made in identifying the genes that are…

Cryo-EM studies of the prothrombinase:prothrombin complex and the protein C anti-coagulant complex

Lead Institution

Saint Louis University

Principal Investigator

Enrico Di Cera

The objective of this study, funded by 2 active NIH grants, is to elucidate the structures of key proteins and their interacting partners of the prothrombinase:prothrombin complex and the protein C…

Visualizing pathways in cells for more accurate biodesign

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

In this Capability Development Focus project, we plan to acquire label-free whole cell three-dimensional (3D) tomograms of the two primary organisms of interest for the FY18 Subcellular SSA thrust…

Opportunities linking omics and structural biology at PNNL: Excelling at Cryo-EM

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

We were recently funded jointly by EMSL and DOE/BER to purchase, install and operate a new Krios G3i cryo-TEM at EMSL. By funding the system jointly, the new microscope would become available to EMSL…

Collaborative Access Team: Pacific Northwest Cryo-EM Center (PNCC)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

PNNL will create and operate the Pacific Northwest Center for Cryo-EM (PNCC), a state-of-the-art national center for cryo-electron microscopy (Cryo-EM) that will rapidly and efficiently collect and…

Accurately predict the spatial arrangement of protein complexes from subtomogram images through physics-informed machine learning

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

Deducing three-dimensional (3D) structures of molecular assemblies from cryo-electron tomography (cryoET) data provides an unprecedented level of detail about biological systems in their native…

Joining Systems Biology and Bioimaging on Same Cells

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

We plan to develop a new capability at EMSL for coupling bioimaging and systems biology analysis of the same exact cells. The use of the highly simplified and smallest free-living eukaryote…

Towards proteome-wide assessment of cellular landscapes

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

We will develop a novel workflow to characterize proteome-wide structural changes of cellular landscapes between different cell states (or cell types). The goal is to bridge the gap between cellular…

KATP channel structures

Lead Institution

Oregon Health & Science University

Principal Investigator

Show-Ling Shyng

ATP-sensitive potassium (KATP) channels couple cell metabolism to membrane excitability and are important for regulating hormone secretion, neuronal activity, vascular tone, and adaptive response to…

Assessment of EUV Atom Probe Tomography for Mass Spectral Analysis of Biomolecules

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Daniel Perea

A Local Electrode Atom Probe (LEAP) Atom utilizing a pulsed, coherent, extreme ultraviolet (EUV) source will be used for the first time for mass-spectral and 3D atomic-scale reconstruction analysis…

Cryo-EM studies of TRP channels

Lead Institution

Duke University

Principal Investigator

Seok-Yong Lee

Transient receptor potential (TRP) channels are a superfamily of calcium-permeable ion channels that are involved in diverse physiological processes. A subset of TRP channels that are known as the…

Virtual Proteomics (ViP): Integrative modeling of protein assemblies in a cell with constraints from proteomic data and reconstructed images from cryo-electron tomography

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

The objective of the proposed research is to advance the spatial knowledge of higher-order protein assemblies in a cell by integrating improved image reconstruction from cryo-electron tomography and…

Dynamic TEM

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Achieving a holistic, molecular-level understanding of the structure, function and mechanism of cellular machinery remains a formidable scientific challenge. While over 100,000 protein structures…

Building an automation-ready multimodal workflow for comprehensive 3D characterization of intact soil structures.

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Odeta Qafoku

3-dimensional (3D) characterization of intact soil structures (i.e. macro and microaggregates) will provide understandings on how soil physical, mineralogical, biotic heterogeneities control long…

Application of machine learning to APT data to trace nanoscale protein structure

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Daniel Perea

The objective of this project is the development and application of deep learning algorithms aimed at extracting molecular structure within Atom Probe Tomography (APT) data collected from protein…

Tomography of Pseudarthrobacter chlorophenolicus

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

Subcellular structures and macromolecular complexes can be revealed through cryo electron tomography of whole cells. Pseudarthrobacter chlorophenolicus is a soil bacterium which has novel metabolic…

Understanding the structural basis of RFX complex in regulating MHC gene expression

Lead Institution

Oregon Health & Science University

Principal Investigator

Liman Zhang

The Major Histocompatibility complex (MHC) is critical in immune system for T cell maturation and self vs nonself discrimination, thus expression of MHC genes has to be tightly regulated. Active…

Structural Mechanism of Jordan's Syndrome

Lead Institution

University of Wisconsin, Madison

Principal Investigator

Yongna Xing

Protein phosphatase 2A (PP2A) is an essential and major serine/threonine phosphatase in all eukaryotic cells, and regulates multiple cellular processes and signaling events. Deregulation of PP2A and…

Projects