Tujin Shi

Universal internal standard for reproducible accurate quantification of exosomal proteins

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Pancreatic cancer is currently the third leading cause of cancer related to death in the USA with a 5- year survival rate of <5%. Most potentially curable patients present with unresectable…

Quantitative Proteomics-based Prostate Cancer Prediction Models for African American and Caucasian American Patients

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

In this research project, the Pacific Northwest National Laboratory (PNNL) research team will support the project by conducting quantitative proteomics analysis using mass spectrometry to identify…

Multiplex Mass Spectrometric Protein Assays for Obesity

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

The prevalence of overweight and obesity has increased dramatically in recent decades in both children and adults, reaching an epidemic proportion. In order to gain new insights into the…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Alternative RNA splicing events contribute to the onset of islet dysfunction in type 1 diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ernesto Nakayasu

Type 1 diabetes (T1D) is a results from the autoimmune destruction of pancreatic β cells, which are the cells responsible for insulin production and secretion in the boby. Despite T1D is primarily…

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

High-Throughput Proteomics: Applications and Development (PNNL Scope #40601 and #43488)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Gordon Anderson

This project involves the application and development of high-throughput proteomics and quantitative measurement capabilities within biological systems. More so, application measurements will be…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Cancer Moonshot - Framingham

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Cancer Moonshot -Framingham projects is to perform multiplexed, quantitative LC-MS analysis of longitudinal serum samples associated with head and neck cancer, B-cell lymphoma,…

Early Breast Cancer Biomarkers Verification (PNNL Scope #47174, RD Smith's EIF project)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

Our overall objective for studies performed in Years 4 to 6 is to contribute to the assembly of a highly confident protein panel for the early detection of breast cancer by applying the PNNL…

Developing a strategy to identify & validate pharmacodynamic kinase inhibitorbiomarkers

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Keqi Tang

The primary goal of the project is to develop mass spectrometry (MS) based assays capable of globally quantifying changes in kinase-specific phosphorylation stoichiometry. The specific work related…

A streamlined platform for phosphoproteome mapping of human tissues

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Recent technological advances in genomics, transcriptomics, and proteomics allow for rapid generation of comprehensive 3D-human tissue maps for biomolecules DNAs, RNAs, and proteins at the single…

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

David Camp

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

CPTAC Proteome Characterization Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Proteome Characterization Center (PCC) is to integrate genomic information with proteomic technologies to comprehensively characterize the proteome and post…

A scalable mass spectrometry platform for proteome mapping of brain tissues

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

The brain is the most complex organ in the mammalian body. Bulk analysis obscures heterogeneity of cell types present even in the smallest brain regions. Multi-omics single-cell resolution 3D…

Development of a Universal Multiplex Assay System fot High-Throughput Biological Applications

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Recent advances in genomics, proteomics, and metabolomics make the 'omics' technologies powerful discovery-based tools for identifying candidate biomarkers for human diseases; however, it…

Multi-marker Urine Test for Detection of Aggressive Prostate Cancer

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

In prostate cancer disease management over-diagnosis and over-treatment have increasingly become major public concerns. Prostate cancer is primarily detected by serum PSA screening followed by a…

Targeted proteomics technology for accurate quantitative single-cell proteomics

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tujin Shi

Multi-omics characterization of a broad spectrum of small subpopulations of cells between tumors and within individual tumors at the single-cell resolution is crucial to achieve understanding of a…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Cancer Moonshot - Framingham

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Cancer Moonshot -Framingham project is to apply multiplex quantitative LC-SRM-MS assays for comprehensive, quantitative analysis of longitudinal serum samples associated with head…

Center for Application of Advanced Clinical Proteomic Technologies for Cancer (NCI U24; 59963)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Clinical Proteome Characterization Center (PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype using detailed…

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Validation and Advanced Development of Emerging Technologies

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Keqi Tang

Develop mass spectrometry (MS) based assays for quantitative biomarker validation with sensitivity and specifically comparable to or better than antibody based immunoassays. The new liquid…

Cancer Moonshot - Framingham

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Cancer Moonshot -Framingham project is to continue finishing analysis of ~1300 serum samples with applying multiplex quantitative LC-SRM-MS assays for comprehensive, quantitative…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

CPTAC Proteome Characterization Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Proteome Characterization Center (PCC) is to integrate genomic information with proteomic technologies to comprehensively characterize the proteome and post…

Development of a Universal Multiplex Assay System fot High-Throughput Biological Applications

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Recent advances in genomics, proteomics, and metabolomics make the 'omics' technologies powerful discovery-based tools for identifying candidate biomarkers for human diseases; however, it…

Multi-marker Urine Test for Detection of Aggressive Prostate Cancer

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

In prostate cancer disease management over-diagnosis and over-treatment have increasingly become major public concerns. Prostate cancer is primarily detected by serum PSA screening followed by a…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Multiplex Mass Spectrometric Protein Assays for Obesity

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

The prevalence of overweight and obesity has increased dramatically in recent decades in both children and adults, reaching an epidemic proportion. In order to gain new insights into the…

Center for Application of Advanced Clinical Proteomic Technologies for Cancer (NCI U24; 59963)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Richard Smith

The overall objective of the PNNL Clinical Proteome Characterization Center (PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype using detailed…

Pathways and Critical Regulators of Early Beta-cell Dysfunction in Type 1 Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Type 1 diabetes (T1D) is a complex autoimmune disease. Recent clinical studies show that pancreatic beta-cell dysfunction (e.g., a decline of first phase insulin release) occurs several years before…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Integrated multi-omics analysis of multiple myeloma

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

In this project, integrated quantitative global proteomics and phosphoproteomics analysis of multiple myeloma cases (unsorted and sorted cell types) will be performed using multiplexed tandem mass…

Regulatory Networks and Biomarkers of Beta-cell Dysfunction and Apoptosis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

In type 1 diabetes (T1D) research there remains a critical need for having a systems level understanding of the early stage molecular mechanisms that trigger the specific destruction of pancreatic β…

Reverse Sensitivity Analysis for Identifying Predictive Proteomics Signatures of
Cancer

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Cancer is a complex disease in which genetic disruptions in cell signaling networks are known to play a significant role. A major aim of cancer systems biology is to build models that can predict the…

Projects