Vivian Lin

Accelerating the identification of novel secondary metabolites in bioenergy plant root exudates using microcrystal electron diffraction (MicroED)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

Project type

Cryo-EM Only

Small molecule metabolites drive inter- and intraspecies communication and dependencies in diverse biological systems, yet a large proportion of these important chemical compounds remain…

Fiber Optic Sensor for Microscale Monitoring of Nitrate and Oxygen in the Rhizosphere

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Torgeson

We propose to develop a fiber sensor capable of continuous, in situ, spatially-resolved, simultaneous nitrate and dissolved oxygen measurements within the rhizosphere in tandem with oxygen optode…

Heracles: In Silico Predictive Tools for Opioid Crisis Intervention

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Katherine Schultz

This project aims to develop software tools to identify and prioritize known and potentially emerging pharmaceutical-based agents and new psychoactive substances (NPS, e.g., variants of illicit drugs…

Predicting adverse biological effects for chemicals of concern through integration of functional profiling with artificial intelligence/machine learning (AI/ML)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We will use thermal proteome profiling (TPP) to identify protein targets and pathways affected by exposure to a chemical of concern (COC). As a next step, we will use an artificial intelligence…

Accelerating the identification of novel secondary metabolites in bioenergy plant root exudates using microcrystal electron diffraction (MicroED)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

Small molecule metabolites drive inter- and intraspecies communication and dependencies in diverse biological systems, yet a large proportion of these important chemical compounds remain…

Identifying & Translating OP Receptors in Animal Models and Humans using ABPP

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Aaron Wright

This EMSL Resource Owner proposal requests use of EMSL resources, owned or co-owned, in order to successfully accomplish the use of activity based protein profiling (ABPP) capabilities to identify…

ABPP of Opioid and Sedative Receptors to Improve Medical Countermeasure Development

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Aaron Wright

This EMSL Resource Owner proposal seeks to utilize Orbitrap MS capabilities for advancing our understanding of opioid and sedative exposures, which could lead to developing safe and efficacious…

Profiling protease activities in sorghum under drought stress and recovery

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We will investigate proteases as signatures of plant drought stress by using in vivo activity-based protein profiling to identify and evaluate the contributions of distinct serine and cysteine…

Spatially resolved chemical proteomics for linking enzymatic activity to omics phenotyping in heterogeneous microbial communities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We will connect chemical probes and nanoPOTS to rapidly identify microbial species and essential protein functions in structured microbial communities. We will use aggregation induced emission (AIE)…

Toward plant spatial hormonomics: multiple phytohormone profiling by derivatization assisted-ionization enhanced targeted MALDI MS imaging

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Dusan Velickovic

Phytohormones are physiologically important small molecules that regulate diverse processes as chemical messengers within plants and in plant communications with environments. However, the complex…

Developing an automation-ready high throughput chemoproteomic workflow

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

We propose to develop a high-throughput (HTP) workflow for activity-based protein profiling (ABPP) or bioorthogonal non-canonical amino acid tagging (BONCAT) samples. This HTP chemoproteomic sample…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Biosystems Analysis for Determining the Impact of Nutrient Uptake on Autotroph-Heterotroph Interactions within Cyanobacterial Consortia

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Stephen Callister

Project type

Large-Scale EMSL Research

The exchange of carbon and other nutrients between microorganisms is a key factor in the establishment of microbial interactions and trophic levels. Depending upon the nutrient, the establishment of…

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Linking phosphorus and carbon in rhizosphere nutrient cycling

Lead Institution

Michigan State University

Principal Investigator

James Moran

Project type

FICUS Research

Plant root exudates provide a significant carbon (C) source for rhizosphere microbial communities. In return, these communities help mobilize nutrients needed for plant growth. For instance,…

Identification of plant host and microbe model system for study of rhizophagy cycle

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vivian Lin

Rhizophagy (“root eating”) is a process by which plants actively uptake soil microbes into their roots and use oxidative degradation to extract micronutrients from the cells. This phenomenon has been…

Development of in vitro glycosylation toolkit to study eukaryotic glycoproteins

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Irina El Khoury

When working with glycoproteins from native sources, biochemical analysis is often hampered by sample heterogeneity and glycan-postprocessing structural diversity. It is therefore desirable to create…

Phosphorus and Carbon Cycling Directed by Spatial Plasticity in Root Exudation

Lead Institution

Michigan State University

Principal Investigator

James Moran

Project type

Exploratory Research

Plant root exudates provide a significant carbon (C) source for rhizosphere microbial communities. In return, these communities help mobilize nutrients needed for plant growth. For instance,…

Tracing rhizosphere carbon exchange processes and nutrient interactions

Lead Institution

Michigan State University

Principal Investigator

James Moran

Rhizodeposition represents the largest flux of photosynthetically derived organic matter into soil for most terrestrial systems and, in so doing, serves as a major point of connection between…

Projects