Weijun Qian

Omics Data Generation Center (ODGC) for the Acute to Chronic Pain Signatures (A2CPS)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

the overall objective of this PNNL-Pittsburgh Omics Data Generation Center component (PP-ODGC) is to develop and implement high throughput omics-based assays for quantifying a broad and diverse set…

Enhancing biopreparedness through a model system to understand the molecular mechanisms that lead to pathogenesis and disease transmission

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Margaret Cheung-Wyker

The science of biopreparedness to counter biological threats hinges on understanding the fundamental principles and molecular mechanisms that lead to pathogenesis and disease transmission. Our vision…

Pathways and Critical Regulators of Early Beta-cell Dysfunction in Type 1 Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Type 1 diabetes (T1D) is a complex autoimmune disease. Recent clinical studies show that pancreatic beta-cell dysfunction (e.g., a decline of first phase insulin release) occurs several years before…

Integrated risk assessment and molecular characterization of pulmonary response
to e-cigarette exposure

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

E-cigarette (e-cig) aerosol/vapor is a complex mixture of the original components of the e-liquid (propylene glycol (PG), vegetable glycerol (VG), nicotine, water, and flavoring additives), and of…

Multiplex Mass Spectrometric Protein Assays for Obesity

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

The prevalence of overweight and obesity has increased dramatically in recent decades in both children and adults, reaching an epidemic proportion. In order to gain new insights into the…

Spatially Resolved Proteomic studies of Brains Obtained from Control Mice and Treated Mice Simulating Parkinson's Disease

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Research on the three-dimensional transcriptome of voxelated mice brains has demonstrated that significant RNA expression differences are detectable when comparing the brain transcriptomes of…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

PNNL Proteomics Chemical Analysis Center for the MoTrPAC (ProMoTr)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Although the health benefits of physical activity in prevention and mitigation of many chronic diseases have been documented for decades, the molecular mechanisms mediating these health benefits are…

Proteomic Studies of Inflammation and the Host Response to Injury

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Inflammation and The Host Response to Injury, a large scale collaborative research program, is currently funded as a "glue" grant by the National Institute of General Medical Sciences …

Proteomic Studies of type 2 diabetes using knockout models

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

A central goal of diabetes research (type 1 and type 2) is to generate large numbers of functional islets or beta-cells for replacement therapy. Therefore, a fundamental knowledge of mechanisms and…

Center for Application of Advanced Clinical Proteomic Technologies for Cancer (NCI U24; 59963)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Richard Smith

The overall objective of the PNNL Clinical Proteome Characterization Center (PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype using detailed…

Cancer Moonshot - Framingham

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Cancer Moonshot -Framingham project is to apply multiplex quantitative LC-SRM-MS assays for comprehensive, quantitative analysis of longitudinal serum samples associated with head…

Title: Advanced Proteomics and Metabolomics Studies of Type 2 Diabetes and Pre-Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Abstract: In this five year (R21/R33) project we will apply advanced proteomic and metabolomic nanoflow liquid chromatography-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry…

Early Breast Cancer Biomarkers Verification (PNNL Scope #47174, RD Smith's EIF project)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

Our overall objective for studies performed in Years 4 to 6 is to contribute to the assembly of a highly confident protein panel for the early detection of breast cancer by applying the PNNL…

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Agile BioFoundry

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

This proposed EMSL Resource Owner project, is a sub-project supporting two DOE-EERE-BETO supported PNNL projects: FY16-17 four-national laboratory “Agile BioFoundry” (ABF) seed project and a larger…

Title: Advanced Proteomics and Metabolomics Studies of Type 2 Diabetes and Pre-Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Abstract: In this five year (R21/R33) project we will apply advanced proteomic and metabolomic nanoflow liquid chromatography-Fourier transform ion cyclotron resonance (FTICR) mass spectrometry…

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

David Camp

Collaborative research projects in the PNNL NIH NCRR P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Regulatory Networks and Biomarkers of Beta-cell Dysfunction and Apoptosis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

In type 1 diabetes (T1D) research there remains a critical need for having a systems level understanding of the early stage molecular mechanisms that trigger the specific destruction of pancreatic β…

PNNL Proteomics Chemical Analysis Center for the MoTrPAC (ProMoTr)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

Although the health benefits of physical activity in prevention and mitigation of many chronic diseases have been documented for decades, the molecular mechanisms mediating these health benefits are…

Quantitative Characterization of protein post-translational modifications using mass spectrometry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Post-translational protein modifications such as phosphorylation and oxidative modifications play essential roles in the regulation of a variety of biological processes. Increasing evidence suggests…

Development of a Universal Multiplex Assay System fot High-Throughput Biological Applications

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Recent advances in genomics, proteomics, and metabolomics make the 'omics' technologies powerful discovery-based tools for identifying candidate biomarkers for human diseases; however, it…

Enabling Agile Biomanufacturing with Leading Edge Analytical Biology

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Mark Butcher

This proposed EMSL User project supports a larger multi-lab "Agile Biomanufacturing" project (LBNL, PNNL, SNL). Through the EMSL’s leading edge analytical biology, GC and LC mass…

OHSU-PNNL NORTHWEST CO-LABORATORY FOR INTEGRATED OMICS

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

An essential component of the OHSU-PNNL Northwest CoLaboratory for Integrated Omics (CoLab) is the collaborative pursuit of joint research projects that leverage the compelling biomedical model…

Pilot Study on Examination of LIRKO Islets and Serum Using Quantitative Proteomic Approaches

Lead Institution

Joslin Diabetes Center

Principal Investigator

Rohit Kulkarni

A central goal of diabetes research (type 1 and type 2) is to generate large numbers of functional islets or &#61538;-cells for replacement therapy. Therefore, a fundamental knowledge of…

Proteomic Studies of Inflammation and the Host Response to Injury

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Inflammation and The Host Response to Injury, a large scale collaborative research program, is currently funded as a "glue" grant by the National Institute of General Medical Sciences …

OHSU-PNNL NORTHWEST CO-LABORATORY FOR INTEGRATED OMICS

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Karin Rodland

An essential component of the OHSU-PNNL Northwest CoLaboratory for Integrated Omics (CoLab) is the collaborative pursuit of joint research projects that leverage the compelling biomedical model…

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Biomarkers for early detection and other stages (PNNL Scope # 46308, RD Smith's Hutch 1 project)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

Project type

Exploratory Research

Development and identification of early detection polypeptide biomarkers from tryptic peptides using a random approach may also include application of PNNL very high pressure capillary LC-FTICR…

Spatial and Temporal Proteomics for Characterizing Protein Dynamics and Posttranslational Modifications

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

The development of technologies for systems biology is considered a vital component of DOE energy and environmental missions for attaining a systems-level understanding of microbes and plants. …

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…

Multi-marker Urine Test for Detection of Aggressive Prostate Cancer

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

In prostate cancer disease management over-diagnosis and over-treatment have increasingly become major public concerns. Prostate cancer is primarily detected by serum PSA screening followed by a…

Global and Targeted Proteomic Identification of oxidative modifications.

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Diana Bigelow

Project type

Exploratory Research

Recent research has provided an emerging picture of the significant role of reactive oxygen/nitrogen species (ROS/RNS) and their associated modification of cellular proteins in cellular signaling…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

Collaborative research projects in the PNNL NIH P41 Biomedical Technology Research Center 'Proteomics research resource for integrative biology'

Multiplex Mass Spectrometric Protein Assays for Obesity

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

The prevalence of overweight and obesity has increased dramatically in recent decades in both children and adults, reaching an epidemic proportion. In order to gain new insights into the…

Technology development in support of NCRR

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Nancy Colton

The Pacific Northwest National Laboratory (PNNL) has built a facility that already stands at the forefront of proteomic research. Moreover, the Center is uniquely poised for future developments. We…

Urinary Proteome Monitoring for Transplant Injury

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

David Camp

We propose to achieve the objective of identifying novel urinary biomarkers for selected categories of renal transplant injury in two phases. We will first employ a discovery phase to comprehensively…

EDRN Biomarker Reference and Resource Center with the Department of Energy

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The purpose of the Early Detection Research Network (EDRN) Biomarker Reference and Resource Center (BRRC) is to develop a multiplex quantitative MS-based method, based on LC-SRM-MS for simultaneous…

Integrated risk assessment and molecular characterization of pulmonary response
to e-cigarette exposure

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

E-cigarette (e-cig) aerosol/vapor is a complex mixture of the original components of the e-liquid (propylene glycol (PG), vegetable glycerol (VG), nicotine, water, and flavoring additives), and of…

Collaborative Research Projects Supported by the NCRR P41 Resource Center

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

David Camp

Project type

Large-Scale EMSL Research

The Proteomics Resource Center for Integrative Biology at PNNL was established in 2003 and builds on the unique instrumentation and the infrastructure provided by PNNLs EMSL facility. The mission of…

Center for Application of Advanced Clinical Proteomic Technologies for Cancer (NCI U24; 59963)

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Tao Liu

The overall objective of the PNNL Clinical Proteome Characterization Center (PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype using detailed…

Agile BioFoundry

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Jon Magnuson

This proposed EMSL Resource Owner project, is a sub-project supporting two DOE-EERE-BETO supported PNNL projects: FY16-17 four-national laboratory “Agile BioFoundry” (ABF) seed project and a larger…

Pathways and Critical Regulators of Early Beta-cell Dysfunction in Type 1 Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Type 1 diabetes (T1D) is a complex autoimmune disease. Recent clinical studies show that pancreatic beta-cell dysfunction (e.g., a decline of first phase insulin release) occurs several years before…

Proteomic Studies of Inflammation and the Host Response to Injury

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Inflammation and The Host Response to Injury, a large scale collaborative research program, is currently funded as a "glue" grant by the National Institute of General Medical Sciences …

Validation and Advanced Development of Emerging Technologies

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Keqi Tang

Develop mass spectrometry (MS) based assays for quantitative biomarker validation with sensitivity and specifically comparable to or better than antibody based immunoassays. The new liquid…

OHSU-PNNL NORTHWEST CO-LABORATORY FOR INTEGRATED OMICS

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Karin Rodland

An essential component of the OHSU-PNNL Northwest CoLaboratory for Integrated Omics (CoLab) is the collaborative pursuit of joint research projects that leverage the compelling biomedical model…

Omics Data Generation Center (ODGC) for the Acute to Chronic Pain Signatures (A2CPS)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jon Jacobs

the overall objective of this PNNL-Pittsburgh Omics Data Generation Center component (PP-ODGC) is to develop and implement high throughput omics-based assays for quantifying a broad and diverse set…

Isoform-Specific Quantitative Proteomics Applying N-terminal Enrichment and informatics deconvolution

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

For higher eukaryotic organisms, a single gene can produce multiple protein products (or isoforms) by various mechanisms, such as alternative splicing and post-translational proteolytic processing. …

Pan-Omics Technology Development, Implementation, and Application to GSP Systems Biology Research

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Joshua Adkins

The BER Genome Science Program aims to understand complex biological and environmental systems across spatial and temporal scales to develop predictive knowledge relevant to DOE mission challenges…