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Identification of Post-translational Modifications and Protein Complexes under Conditions of Oxidative Stress


EMSL Project ID
10197

Abstract

This proposal falls under the NIH National Center for Research Resources at PNNL.
Our goal is to understand the mechanisms underlying observed alterations in calcium homeostasis and the oxidation and nitration of intracellular proteins that occur under a range of biological pathologies, including biological aging. In this work we will focus on stress-response signaling pathways in mouse macrophages involving the critical calcium regulatory protein calmodulin (CaM). The mass spectrometry tools available to us will be used to 1) identify CaM binding partners and their involvement in signaling pathways 2) investigate the role of nitrated tyrosine in the regulatory processes, using CaM as a model protein and 3) explore the use of CaM as a biosensor to differentiate between types of oxidative stress.

Project Details

Project type
Exploratory Research
Start Date
2004-07-27
End Date
2007-06-28
Status
Closed

Team

Principal Investigator

Thomas Squier
Institution
Western University of Health Sciences

Team Members

Colette Sacksteder
Institution
Pacific Northwest National Laboratory