Slow-MAS NMR methodology developments
EMSL Project ID
10896
Abstract
At PNNL methodologies are under development to improve the resolution of 1H NMR metabolite spectra in intact biological objects (cells, organs, tissues, and even live animals) in a non- or minimally invasive way. This is accomplished by combining special R.F. pulse sequences with a slow or ultra-slow magic angle spinning (MAS) of the object. Although the initial results look very promising and clearly demonstrate feasibility, much more work is needed to further optimize the slow-MAS methodologies and make them suitable for broad applications in biomedical and biochemical research. In the proposed work two of these methods, phase-adjusted spinning sidebands (PASS) and phase-corrected magic angle turning (PHORMAT) will be further evaluated and improved. It is anticipated that if the improvements are successful, the slow-MAS methodology will become a new unique medical capability for, e.g., studies of metabolic activities, molecular diagnostics, therapy response evaluations and drug testing in cells, tissues, and even live animals. Also, the slow-MAS methodology has been patented and the improved methodologies may result in additional support from MRI manufacturers and the pharmaceutical industry. Moreover, slow-MAS NMR will be integrated in several new research proposals that will be submitted to NIH, DOE, and possibly other funding agencies. Finally, the project will result in a new unique EMSL capability, so that the technology will become available to outside users.
Project Details
Project type
Capability Research
Start Date
2004-12-29
End Date
2007-07-18
Status
Closed
Released Data Link
Team
Principal Investigator