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Shew strains

EMSL Project ID


Metal reducing members of the Shewanella genus carry out a variety of processes important to the U.S. department of Energy (DOE) missions including the bioremediation of metals (Fe, Mn, Cr) and radionuclides (U, Tc, Pu) and are important in carbon cycling in a range of aquatic and sedimentary environments. Shewanella are ecologically successful in a variety of environments including freshwater lakes and sediments, different depths and at redox interfaces of the water columns of the Black, Baltic and North Seas. An extensive genetic diversity was recovered in the five sequenced Shewanella strains and the Shewanella genomic scaffolds from the Sargasso sea.
Recently, the JGI has set to sequence 7 new Shewanella species, 5 of which are currently closed. The purpose of this proposal is to use new technology based on the combination of global tryptic digestion, high-resolution liquid chromatography and tandem mass spectrometry and high field FTICR mass spectrometry to help construct and validate the annotation of these organisms and define protein expression patterns of the organism cultured under various conditions.

Project Details

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Principal Investigator

Mary Lipton
Environmental Molecular Sciences Laboratory